Unlock instant, AI-driven research and patent intelligence for your innovation.

Magnetic bead presorting blood collection tube and pretreatment technology of blood DNA using same

A technology of blood collection tubes and prepackaging, applied in the field of molecular biology, can solve the problems of high cost, limited storage time, long transportation time, etc., to ensure stability and integrity, good stability and integrity, and simple method and efficient effect

Inactive Publication Date: 2016-06-15
SUZHOU ENRICHING BIOTECH CO LTD
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional method has great limitations in this process: 1. Currently used for DNA, especially cfDNA and ctDNA detection, blood sample collection, transportation and storage require the use of special blood collection tubes, which are added to prevent blood cells from The cost of broken protective agents, protective agents to prevent DNA degradation, and anticoagulants, etc., is high. Generally speaking, the cost of each blood collection tube is about 200 yuan; 2. The storage and transportation conditions in the later period are demanding, and the original blood samples It is not easy to store, and the storage time is limited. The longer the transportation time will cause more DNA degradation, which will lead to a decrease in the integrity, stability and quantity of DNA extraction.
If blood samples are collected in remote areas, such as mountainous and western areas, due to the long distance and long transportation time, it is difficult to implement even with expensive blood collection equipment
3. If the blood sample needs to be frozen and transported, the repeated freezing and thawing of the blood sample will greatly damage the stability of the DNA in the blood sample and affect the test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment is a magnetic bead pre-packaged blood collection tube and its method for convenient blood DNA pretreatment (including: rough extraction, transportation and storage), and the extraction of genomic DNA in blood includes the following steps:

[0032] Step 1), collect 1mL fresh blood sample into the blood collection tube that has been preloaded with 10mg magnetic beads and 3mL buffer, and mix it upside down;

[0033] The buffer in step 1) includes the following components: 5M guanidine isothiocyanate, 50mM sodium chloride, 0.1% Tween-20, 100mM urea, 80mM Tris-HCl, 20mM EDTA, pH 6-8.

[0034] Step 2), the above-mentioned blood collection tube is vortexed or shaken on a shaker at room temperature for 5-10 minutes to complete the combination of blood genomic DNA and magnetic beads;

[0035] Step 3), place the blood collection tube in the magnetic stand device, magnetically separate for 30-90 seconds, and pour the liquid substance after the magnetic beads are co...

Embodiment 2

[0041] This embodiment is a magnetic bead prepackaged blood collection tube and its method for convenient blood DNA pretreatment, which is aimed at extracting genomic DNA in blood, including the following steps:

[0042] Step 1), take 5mL fresh blood sample into the blood collection tube that has been preloaded with 60mg magnetic beads and 16mL buffer, and mix it upside down;

[0043] The buffer in step 1) includes the following components: 6M guanidine hydrochloride, 60mM sodium chloride, 0.2% Tween-20, 50mM Tris-HCl, 10mM EDTA, pH 6-8.

[0044] Step 2), the above-mentioned blood collection tube is vortexed or shaken on a shaker at room temperature for 5-10 minutes to complete the combination of blood DNA and magnetic beads;

[0045] Step 3), place the blood collection tube in the magnetic stand device, magnetically separate for 30-90 seconds, and pour the liquid substance after the magnetic beads are completely absorbed;

[0046] Step 4), add 18mL of cleaning solution to th...

Embodiment 3

[0051] This embodiment is a magnetic bead prepackaged blood collection tube and its method for convenient blood DNA pretreatment, which is aimed at extracting cfDNA in blood, including the following steps:

[0052] Step 1), take 1mL fresh blood sample into the blood collection tube that has been preloaded with 10mg magnetic beads and 4mL buffer, and mix it upside down;

[0053] The buffer solution in step 1) includes the following components: guanidine isothiocyanate of 4M, sodium chloride of 60mM, 0.4% Tritonx-100, ethanol of 75% volume ratio, 80mM Tris-HCl, 5mM EDTA, pH value 6~ 8.

[0054] Step 2), the above-mentioned blood collection tube is vortexed or shaken on a shaker at room temperature for 5-10 minutes to complete the combination of blood DNA and magnetic beads;

[0055] Step 3), place the blood collection tube in the magnetic stand device, magnetically separate for 30-90 seconds, and pour the liquid substance after the magnetic beads are completely absorbed;

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a magnetic bead presorting blood collection tube and a pretreatment technology of blood DNA using the same. Magnetic beads with superparamagnetism are presorted in a commercially available common blood collection tube, and the surfaces of the magnetic beads are modified by a group with an enrichment effect on DNA. In the scheme, some steps in the traditional whole process of blood sample collection, blood sample transport and laboratory extraction of blood DNA are cut and integrated again; and after the blood sample collection, the combination of target DNA and magnetic beads can be realized in the blood collection tube so as to finish a step of crude extraction of DNA. Traditional blood sample storage and transport are transformed into DNA sample storage and transport; the DNA sample storage and transport can be realized in a normal-temperature environment, and harsh conditions such as cold-chain transport for blood samples are avoided; and moreover, compared with direct transport of blood samples, the stability and integrity of DNA can be guaranteed better, the phenomenon of DNA degradation frequently occurring in long-time transport of blood samples is avoided, and the method is simpler and more convenient and efficient.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a magnetic bead prepackaged blood collection tube and a process method for conveniently performing pretreatment such as DNA crude extraction, storage and transportation on blood samples by using the same. Background technique [0002] With the rapid development of genomics and gene sequencing technology, especially the development of first-generation gene sequencing technology to second-generation gene sequencing technology, the time and cost required for gene sequencing have been greatly reduced. Precision medicine based on gene sequencing is gradually becoming a basic means in the fields of major disease diagnosis, personalized medicine, fetal genetic disease analysis, and individual identification. One of the most important sources of human DNA is blood, such as: genomic DNA present in blood cells, free DNA (cfDNA) present in blood but free from blood cells, DNA (ctD...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12N15/10
CPCC12M23/08C12N15/1013C12Q2563/143C12Q2563/149
Inventor 雷波张佳斌吴巧曲峰尚春庆
Owner SUZHOU ENRICHING BIOTECH CO LTD