Method for determining high oleic acid in brassica napus on basis of BnaLCR78 gene

A technology of Brassica napus and a determination method, which is applied in the directions of biochemical equipment and methods, microorganism determination/inspection, etc., can solve the problems of impracticality, high modeling cost, expensive maintenance cost of instruments and the like

Inactive Publication Date: 2016-06-15
SICHUAN AGRI UNIV
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Problems solved by technology

The near-infrared spectrum region is consistent with the combined frequency of the vibration of hydrogen-containing groups (O-H, N-H, C-H) in organic molecules and the absorption region of multiple levels of frequency multiplication. By scanning the near-infrared spectrum of the sample, the hydrogen-containing groups of organic molecules in the sample can be obtained. The disadvantages are as follows: 1. A large number of representative samples with known chemical values ​​are required to build a model
Thus, the analysis of small batches of samples using near-infrared becomes impractical
2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.
3. The model is not universal, and the model of each instrument is different, which increases the limitation of use
4. The cost of modeling is high and the cost of testing is high
Disadvantages: In the direct qualitative analysis of components, known s...

Method used

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  • Method for determining high oleic acid in brassica napus on basis of BnaLCR78 gene
  • Method for determining high oleic acid in brassica napus on basis of BnaLCR78 gene
  • Method for determining high oleic acid in brassica napus on basis of BnaLCR78 gene

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Experimental program
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Embodiment 1

[0080] (1) The seeds of the maintainer line 100B of Brassica napus, which is a high-oleic acid material, were subjected to the compound treatment of spaceflight mutagenesis + sodium staggering, and the offspring were stably inherited through multiple generations of cultivation. The oleic acid content was 62.60%, expressed in G. Seeds of naturally occurring high-oleic materials (oleic acid content > 60%) can also be used to replace the seeds of the maintainer line 100B of Brassica napus that have been subjected to space-flight mutagenesis + sodium sulfide compound treatment to carry out this experiment.

[0081] Take the control material of high oleic acid material, that is, the maintainer line 100B of Brassica napus without any treatment, grow in the field in a natural state, and the seeds harvested after maturity have an oleic acid content of 56.57%; expressed as CK.

[0082] Take the seeds of You 821 among the low oleic acid materials, the oleic acid content is <40%, which is...

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Abstract

The invention provides a determining method for fast and conveniently judging whether brassica napus belongs to the rape with high oleic acid content, and in particular discloses a determining method for judging whether the transcription of the BnaLCR78 gene of the brassica napus has variable splicing, namely, the intron of the BnaLCR78 gene has variable splicing, thereby judging whether the brassica napus belongs to the rape with high oleic acid content.

Description

[0001] Technical field: the present invention relates to the field of oil crops, in particular to a method for testing and judging whether Brassica napus belongs to high oleic acid rape. Background technique [0002] Brassica napus is an important type of the three major rapeseed resources, and it is also a typical oil crop widely planted in my country. Its fatty acid composition is divided into saturated fatty acids and unsaturated fatty acids. Unsaturated fatty acids are important for human health and plant stress resistance. and post-injury repair play an important role. According to Peng Qi’s 2011 doctoral dissertation (Peng Qi, Cloning of new genes related to the formation of fatty acid components in Brassica napus, 2011, doctoral dissertation, Hunan Agricultural University, China National Knowledge Network Database / CNKI; The T350078 gene is the No. 78 EST clone fragment in the seed-specific expression library of Brassica napus "Xiangyou 15" about 35 days after flowering. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2539/105
Inventor 李壮余世聪蔺丽丽牛应泽吴永成郭世星杜俊波
Owner SICHUAN AGRI UNIV
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