Method for determining high oleic acid in brassica napus on basis of BnaLCR78 gene

Abstract

The invention provides a determining method for fast and conveniently judging whether brassica napus belongs to the rape with high oleic acid content, and in particular discloses a determining method for judging whether the transcription of the BnaLCR78 gene of the brassica napus has variable splicing, namely, the intron of the BnaLCR78 gene has variable splicing, thereby judging whether the brassica napus belongs to the rape with high oleic acid content.

Description

[0001] Technical field: the present invention relates to the field of oil crops, in particular to a method for testing and judging whether Brassica napus belongs to high oleic acid rape. Background technique [0002] Brassica napus is an important type of the three major rapeseed resources, and it is also a typical oil crop widely planted in my country. Its fatty acid composition is divided into saturated fatty acids and unsaturated fatty acids. Unsaturated fatty acids are important for human health and plant stress resistance. and post-injury repair play an important role. According to Peng Qi’s 2011 doctoral dissertation (Peng Qi, Cloning of new genes related to the formation of fatty acid components in Brassica napus, 2011, doctoral dissertation, Hunan Agricultural University, China National Knowledge Network Database / CNKI; The T350078 gene is the No. 78 EST clone fragment in the seed-specific expression library of Brassica napus "Xiangyou 15" about 35 days after flowering. ...

AI Technical Summary

Problems solved by technology

The near-infrared spectrum region is consistent with the combined frequency of the vibration of hydrogen-containing groups (O-H, N-H, C-H) in organic molecules and the absorption region of multiple levels of frequency multiplication. By scanning the near-infrared spectrum of the sample, the hydrogen-containing groups of organic molecules in the sample can be obtained. The disadvantages are as follows: 1. A large number of representative samples with known chemical values ​​are required to build a model

Thus, the analysis of small batches of samples using near-infrared becomes impractical

2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.

3. The model is not universal, and the model of each instrument is different, which increases the limitation of use

4. The cost of modeling is high and the cost of testing is high

Disadvantages: In the direct qualitative analysis of components, known substances or known data must be compared with the corresponding chromatographic peaks, or combined with other methods (such as mass spectrometry, spectroscopy) to obtain direct and positive results

Disadvantages: Liquid chromatography cannot directly give the qualitative results of unknowns from the chromatogram, but must be qualitatively compared with known standards

When there is no pure substance control, qualitative identification is very difficult. At this time, it is necessary to cooperate with mass spectrometry, infrared and chemical methods. Therefore, the selection of known standards is the key to the reliability of the results. The results obtained by using this method alone are not reliable. Well, most metal salts and substances with poor thermal stability cannot be analyzed yet

Although this shortcoming can be overcome by high-performance liquid chromatography, high-performance liquid chromatography has high analysis costs, expensive instruments, high maintenance costs, and long analysis time

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