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Method for simultaneously detecting multiple sugars and sugar alcohols in dairy products

A dairy product and detection method technology, applied in the field of high performance liquid chromatography detection, can solve the problems such as the ineffective separation of sugars, the decline of the column efficiency of the amino column, and the reduction of the column life, and achieves low machine noise, slow decline in column efficiency, The effect of column bleed reduction

Inactive Publication Date: 2016-06-15
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the analysis of amino column-acetonitrile water system, as the number of detected sugars increases, the column efficiency of the amino column decreases, and some sugars cannot be effectively separated.
In order to increase the resolution, it is necessary to continuously adjust the ratio of acetonitrile to water, but when the ratio of acetonitrile in the organic phase increases to a certain extent, the effective bonding of the amino column will be destroyed, thereby reducing the column life

Method used

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  • Method for simultaneously detecting multiple sugars and sugar alcohols in dairy products
  • Method for simultaneously detecting multiple sugars and sugar alcohols in dairy products
  • Method for simultaneously detecting multiple sugars and sugar alcohols in dairy products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Detection of seven sugars and sugar alcohols in milk samples

[0044](1) Preparation of seven sugar single-standard solutions: Accurately weigh sorbitol, maltitol, xylitol, fructose, glucose, sucrose, and lactose to prepare standard stock solutions, and the standard stock solution concentration of lactose is 100mg ·mL -1 , the concentration of standard stock solution of xylitol, fructose, sorbitol, glucose, sucrose is 150mg·mL -1 , the standard stock solution concentration of maltitol is 200mg·mL -1 , for use; the concentration of the standard series is shown in Table 1.

[0045] Table 1 Seven kinds of sugar standard stock solution concentration and standard series solution concentration

[0046]

[0047] (2) Preparation of standard working solution: Xylitol standard working solution 60mg·mL -1 : Take 150mg·mL -1 Put 4.00mL of xylitol standard stock solution into a 10mL volumetric flask, add water to the volume; fructose standard working solution 60mg...

Embodiment 2

[0060] Embodiment 2: the detection of seven kinds of sugars and sugar alcohols in the pastry sample

[0061] Steps (1) and (2) are the same as in Example 1;

[0062] (3) Sample preparation: Mix the sample thoroughly, take about 5.00g of the sample, add water to dissolve and mix, add 2.5mL zinc acetate 22wt%, 2.5mL potassium ferrocyanide 10.6wt%, mix well, dilute to 50mL, mix After uniformity, centrifuge at 6000r / min, centrifuge at 4°C for 5min, take the supernatant, filter it with a 0.22μm needle filter membrane, and use the filtrate for detection;

[0063] Select the liquid-phase external standard method for quantification, prepare a standard working solution according to a certain concentration, use the concentration of the standard solution and the corresponding monomer peak area signal to make a working curve, and use the formula 1 according to the response peak area and sample volume of the monomer in the sample to be tested. Calculation:

[0064] (1)

[0065] In the...

Embodiment 3

[0073] Embodiment 3: the detection of seven kinds of sugars and sugar alcohols in the candy sample

[0074] Steps (1) and (2) are the same as in Example 1;

[0075] (3) Sample preparation: Mix the sample thoroughly, take about 5.00g of the sample, add water to dissolve and mix, add 2.5mL zinc acetate 22wt%, 2.5mL potassium ferrocyanide 10.6wt%, mix well, dilute to 50mL, mix After uniformity, centrifuge at 6000r / min, centrifuge at 4°C for 5min, take the supernatant, filter it with a 0.22μm needle filter membrane, and use the filtrate for detection;

[0076] Select the liquid-phase external standard method for quantification, prepare a standard working solution according to a certain concentration, use the concentration of the standard solution and the corresponding monomer peak area signal to make a working curve, and use the formula 1 according to the response peak area and sample volume of the monomer in the sample to be tested. Calculation:

[0077] (1)

[0078] In the ...

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Abstract

The invention relates to a method for simultaneously detecting multiple sugars and sugar alcohols in dairy products. The method is an HPLC-RID (high performance liquid chromatography-refractive index detection) method and is characterized in that the chromatographic condition of HPLC is as follows: an amido bond column is adopted as a chromatographic column, a single mobile phase of acetone, water and trithylamine is adopted, and isocratic elution is performed; the column temperature is 70 DEG C; the flow velocity is 0.8 ml / min; the sample size is 10 mu L. The detection method is applicable to the dairy products such as milk, cakes, candy, milk powder and the like. Detection can be performed after samples are treated simply. The method is quick, high in recovery rate, wide in linear range, good in correlation coefficient and low in detection limit and fills up the blank of simultaneous detection of the sugars and the sugar alcohols in the dairy products.

Description

technical field [0001] The invention relates to a method for detecting sugar and sugar alcohols in dairy products such as milk, cakes, candy, and milk powder, and in particular to a method for simultaneously detecting sorbitol, maltitol, xylitol, fructose, glucose, and sucrose in the above-mentioned foods. , High performance liquid chromatography detection method of lactose. Background technique [0002] In the current research on sugar detection methods, there are mainly high performance liquid chromatography (HPLC)-fluorescence detection (FLD), HPLC-refractive index (RID), ion chromatography, high performance liquid chromatography-mass spectrometry (HPLC- MS / MS), high performance gas chromatography-mass spectrometry (GC-MS / MS). Although the HPLC-FLD method has high sensitivity, it has strong selectivity; ion chromatography has good repeatability and accuracy, but sugars can reduce some molecules in the sample on the electrode surface; HPLC-MS / MS method Although the GC-MS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘琳段小娟张丽郝鹏飞徐琴
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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