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Preparation method of highland barley barley green rich in superoxide dismutase

A technology of superoxide and barley green, applied in the field of preparation of highland barley barley green, can solve the problems of high operation requirements and operating equipment requirements, no mention of SOD specific values, high production costs and difficult promotion, etc., and achieves no taste. Grassy smell, good operability, clear and bright solution

Active Publication Date: 2016-06-22
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ultrafiltration-reverse osmosis combined concentration technology adopted by it has high requirements for operation and equipment, and the high production cost is not easy to promote, and the specific value of SOD is not mentioned.
[0005] The descriptions of SOD enzyme content in other existing products are often vague words such as "high", "very high" and "rich". In addition, some barley green products on the market have a strong grassy smell and obvious layering after mixing. Seriously affect eating

Method used

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  • Preparation method of highland barley barley green rich in superoxide dismutase
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  • Preparation method of highland barley barley green rich in superoxide dismutase

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Comparison scheme
Effect test

Embodiment 1

[0153] A preparation method of highland barley green pigment rich in superoxide dismutase is as follows:

[0154] 1) Pretreatment: Harvest 100g of highland barley seedlings up to 20cm in length, remove withered, discolored wheat seedlings, and other weeds, add 2000ml of water to the highland barley seedlings, ultrasonicate twice for 30 minutes each time, and discard the water;

[0155] 2) Sterilization: the pretreated highland barley seedlings are sterilized by saturated steam method, and the sterilization time is 8s;

[0156] 3) Drying and pulverization: vacuum-dry the wheat seedlings after sterilization at a drying temperature of 70° C., and pulverize them to obtain highland barley grass powder;

[0157] 4) SOD extraction and separation: add highland barley seedling powder, 1000ml of phosphate buffer solution with pH=6.5, and 160mg of copper gluconate in a constant temperature stirring tank, soak and extract at 100r / min, 70°C for 15min; filter , collect the filtrate for sub...

Embodiment 2

[0163] A preparation method of highland barley green pigment rich in superoxide dismutase is as follows:

[0164] 1) Pretreatment: Harvest 100g of highland barley seedlings up to 40cm long, remove withered, discolored wheat seedlings, and other weeds, add 2000ml of water to the highland barley seedlings, ultrasonicate twice, each time for 30min, and discard the water;

[0165] 2) Sterilization: sterilize the pretreated barley seedlings by saturated steam method, and the sterilization time is 10s;

[0166] 3) Drying and pulverization: vacuum-dry the wheat seedlings after sterilization at a drying temperature of 80° C., and pulverize them to obtain highland barley grass powder;

[0167] 4) SOD extraction and separation: add highland barley seedling powder, 1200ml of phosphate buffer solution with pH=7.0, and 100mg of copper gluconate in a constant temperature stirring tank, soak and extract at 100r / min, 80°C for 10min; filter , collect the filtrate for subsequent use;

[0168]...

Embodiment 3

[0173] A preparation method of highland barley green pigment rich in superoxide dismutase is as follows:

[0174] 1) Pretreatment: Harvest 100g of highland barley seedlings up to 30cm long, remove withered, discolored wheat seedlings, and other weeds, add 2000ml of water to the highland barley seedlings, ultrasonicate twice for 30 minutes each time, and discard the water;

[0175] 2) Sterilization: sterilize the pretreated barley seedlings by saturated steam method, and the sterilization time is 9s;

[0176] 3) Drying and pulverization: vacuum-dry the wheat seedlings after sterilization at a drying temperature of 60° C., and pulverize them to obtain highland barley grass powder;

[0177] 4) SOD extraction and separation: Add highland barley seedling powder, 800ml phosphate buffer solution with pH=6.0, and 200mg copper gluconate in a constant temperature stirring tank, soak and extract at 100r / min, 60°C for 20min; filter, Collect the filtrate for subsequent use;

[0178] 5) S...

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Abstract

The invention discloses a preparation method of a highland barley barley green rich in superoxide dismutase (SOD). The method includes the steps of pretreatment, sterilization, drying and crushing, extraction and separation of SOD, secondary extraction, concentration and drying, crushing and the like. The method is simple to operate, extraction and concentration are carried out by combining a phosphate buffer solution with copper gluconate and calcium lactate, and the easy-sensitivity substance SOD can be extracted and retained to a greatest extent. The enzyme activity of the highland barley barley green SOD extracted by the method reaches up to 251.6 u / g, has the advantages of fresh mouthfeel, no grass flavor, quick dissolving, clear and bright solution, and uniform and full color and luster, and is a natural and healthy product.

Description

technical field [0001] The invention relates to a preparation method of highland barley green pigment rich in superoxide dismutase, and belongs to the technical field of health food processing. Background technique [0002] Barley chlorophyll is a general term for products processed from wheat seedlings through a certain production process. It is rich in flavonoids, vitamins, minerals, active enzymes and other functional ingredients. Studies have shown that barley green can enhance human immunity, has strong anti-oxidation and anti-aging functions, and can play a role in laxative, lowering blood sugar, lowering blood fat, and promoting gastrointestinal absorption. [0003] Highland barley (Highland barley), commonly known as naked barley, belongs to the grass family and the genus Hordeum. It is a unique species on the Qinghai-Tibet Plateau and the most plateau-specific crop. Fresh barley seedlings have the characteristics of high fiber, high vitamins, low fat, and low sugar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29
Inventor 马宏伟杨文钰魏爱云杜连平孙绪丁
Owner SHANDONG JINHE DRUG RES DEV
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