Method for selectively removing monosaccharides and producing oligosaccharides by using yeast culture

A yeast culture and oligosaccharide technology, applied in the field of bioengineering, can solve the problems of difficult large-scale production, poor separation selectivity, and high calorie consumption, and achieve the effect of overcoming poor separation selectivity, large differences in physical properties, and simple process

Inactive Publication Date: 2016-06-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solvent precipitation method utilizes the difference in solubility between oligosaccharides and monosaccharides. In high-concentration solvents, oligosaccharides are first precipitated to separate them from monosaccharides and oligosaccharides. However, the separation selectivity is poor and a large amount of solvent is consumed. Recycling consumes a lot of heat
Gel chromatography is only suitable for small-scale experiments, the cost is high, and it is difficult to apply to large-scale production

Method used

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  • Method for selectively removing monosaccharides and producing oligosaccharides by using yeast culture
  • Method for selectively removing monosaccharides and producing oligosaccharides by using yeast culture
  • Method for selectively removing monosaccharides and producing oligosaccharides by using yeast culture

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Effect test

Embodiment 1

[0035] Embodiment 1: the acid hydrolysis process of thermogelling polysaccharide

[0036] Thermogel is insoluble in water, but soluble in alkaline solution. Weigh 20g hot gel powder and dissolve in 1000mL1.0mol L -1 In the NaOH solution, stir magnetically for 3-4h until it is fully dissolved. Slowly add 5mol·L -1 The HCl solution is neutralized to neutral, and the neutralization solution is at 8000r·min -1 Centrifuge for 10 min, and wash the precipitate with water 2-3 times. Resuspend the precipitate in 80°C hot water, add concentrated hydrochloric acid to a final concentration of 0.5mol L -1 , stirred and reacted at 80° C. for 60 min, added NaOH solution to neutralize to neutral, cooled the reaction liquid to room temperature, and obtained the partial hydrolyzate of curdlan.

Embodiment 2

[0037] Embodiment 2: the acid hydrolysis process of gellan gum polysaccharide

[0038] Prepare 1000mL of 20g / L gellan gum polysaccharide solution, the reaction temperature is 70°C, add H 2 SO 4 solution to a final concentration of 0.5 mol L -1 , hydrolyzed at a constant temperature for 4 hours under stirring conditions, and then neutralized to neutral with NaOH solution to obtain a partial hydrolyzate of gellan gum polysaccharide.

Embodiment 3

[0039] Embodiment 3: the enzymatic hydrolysis process of thermocurdlan

[0040] Thermogel is insoluble in water, but soluble in alkaline solution. Weigh 20g hot gel powder and dissolve in 1000mL1.0mol L -1 In the NaOH solution, stir magnetically for 3-4h until it is fully dissolved. Slowly add 5mol·L -1 The HCl solution is neutralized to neutral, and the neutralization solution is at 8000r·min -1 Centrifuge for 10 min, and wash the precipitate with water 2-3 times. Resuspend the pellet at 50°C, pH 6.0, 0.025mol L -1 In sodium phosphate buffer, 1000 mL of thermal gel ultrafine suspension was obtained. Add 200mL of 570U / mL β-1,3 glucan hydrolase crude enzyme solution (Journal of Food and Biotechnology, 2015, 34(11):1146-1154.), react at 50°C for 3h, after the reaction The temperature was raised to 80° C., and the reaction was terminated for 10 minutes to obtain a partial hydrolyzate of thermogelling polysaccharide.

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Abstract

The invention discloses a method for selectively removing monosaccharides and producing oligosaccharides by using a yeast culture, belonging to the technical field of bioengineering. Since the oligosaccharides and monosaccharides have small differences in physical properties, the conventional separation process can not easily separate the monosaccharides and oligosaccharides. After the polysaccharides are partially hydrolyzed, the obtained hydrolysate contains oligosaccharides and monosaccharides; and in order to obtain the functional oligosaccharides with higher purity, the monosaccharides need to be separated and removed. The microbial reaction is introduced into the biological separation process, the substrate consumption selectivity for the yeast culture is utilized to remove the monosaccharide components and maintain the oligosaccharide components, thereby enhancing the purity of the oligosaccharides and having obvious advantages.

Description

technical field [0001] The invention relates to a method for selectively removing monosaccharides to produce oligosaccharides by using yeast culture, and belongs to the technical field of bioengineering. Background technique [0002] Oligosaccharides are a class of biologically active substances with important physiological functions, usually composed of one or more different monosaccharides linked by glycosidic bonds, and are low-molecular carbohydrates with a degree of polymerization of 2-10. Functional oligosaccharides refer to a class of oligosaccharides that are not digestible, have special physiological functions, and are beneficial to human and animal health. The main components of prebiotics currently on the market are functional oligosaccharides. Functional oligosaccharides have different physiological activities and functions, mainly preventing dental caries, promoting the growth of intestinal beneficial microorganisms, promoting the digestion and absorption of nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12R1/865C12R1/84C12R1/72C12R1/73
CPCC12P19/00
Inventor 郑志永王剑詹晓北朱莉张洪涛
Owner JIANGNAN UNIV
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