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Preparation method of glycosylation enteromorpha oligosaccharide for reducing glucose level and regulating intestinal flora

A technology to regulate intestinal flora and Enteromorpha oligosaccharides, which is applied in the direction of medical preparations containing active ingredients, digestive system, pharmaceutical formulas, etc., can solve the problem of glycosylated Enteromorpha oligosaccharides that have not yet been seen to lower blood sugar intestinal probiotics Proliferation function research and other issues, to achieve the effect of increasing commodity value and economic added value, simple operation, and improving the original activity

Active Publication Date: 2016-06-22
清枫链食苏打饮品(吉林)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few reports on the research on functional Enteromorpha oligosaccharides, and there are no related reports or patents on the research on the function of glycosylated Enteromorpha oligosaccharides to lower blood sugar and promote the proliferation of intestinal probiotics

Method used

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  • Preparation method of glycosylation enteromorpha oligosaccharide for reducing glucose level and regulating intestinal flora
  • Preparation method of glycosylation enteromorpha oligosaccharide for reducing glucose level and regulating intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Take the dry, ultrafine pulverized Enteromorpha powder passed through a 80-mesh sieve, add an ethanol solution with a mass concentration of 60% at a weight-to-volume ratio (g / mL) of 1:20, and extract with 200W ultrasound at 60°C for 1 hour. After the extraction is completed, filter, repeat the above steps for 2 extractions on the filter residue, combine the extracts, mix the obtained filtrates, concentrate under reduced pressure to 3% of the original volume, freeze-dry in a vacuum, then pulverize, and pass through a 50-mesh sieve;

[0018] (2) Add distilled water to the powder obtained in step (1), wherein the powder mass to distilled water volume ratio is (g / mL) 1:10, stir and redissolve, and use trifluorotrichloroethane method to remove protein 3 times;

[0019] (3) Pass the solution obtained in step (2) through the column filled with HPD-826 macroporous adsorption resin, and the eluent is further passed through the non-polar adsorbent activated carbon. mL) 1:30 t...

Embodiment 2

[0026] (1) Take the dry, ultrafine pulverized, 100-mesh sieve powder of Enteromorpha enteromorpha, add 80% ethanol solution at a weight-to-volume ratio (g / mL) of 1:30, and extract with 400W ultrasound at 90°C for 1.5 hours. After the extraction is completed, filter, repeat the above steps for 3 extractions on the filter residue, combine the extracts, mix the obtained filtrates, concentrate under reduced pressure to 8% of the original volume, freeze-dry in a vacuum, then pulverize, and pass through an 80-mesh sieve;

[0027] (2) Add distilled water to the powder obtained in step (1), wherein the powder mass to distilled water volume ratio is (g / mL) 1:20, stir and redissolve, and use the Sevag method to remove protein 5 times;

[0028] (3) Pass the solution obtained in step (2) through HPD-826 macroporous adsorption resin-packed column, and the eluent is further passed through the non-polar adsorbent activated carbon, and the amount of activated carbon used and the eluent are in ...

Embodiment 3

[0035] (1) Take the powder of Enteromorpha enteromorpha that has been dried, ultrafinely pulverized and passed through a 90-mesh sieve, add ethanol solution with a mass concentration of 75% at a weight-to-volume ratio (g / mL) of 1:26, and extract with 300W ultrasound at 75°C for 1.3 hours. After the extraction is completed, filter, repeat the above steps for 3 extractions on the filter residue, combine the extracts, mix the obtained filtrates, concentrate under reduced pressure to 5% of the original volume, freeze-dry in a vacuum, then pulverize, and pass through a 70-mesh sieve;

[0036] (2) Add distilled water to the powder obtained in step (1), wherein the powder mass to distilled water volume ratio is (g / mL) 1:16, stir and redissolve, and use trichloroacetic acid method to remove protein 4 times;

[0037] (3) Pass the solution obtained in step (2) through the column filled with HPD-826 macroporous adsorption resin, and the eluent is further passed through the non-polar adsor...

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Abstract

The invention discloses a preparation method of glycosylation enteromorpha oligosaccharide for reducing glucose level and regulating intestinal flora. The method includes the steps of smashing enteromorpha in a superfine mode after drying, conducting backflow extracting through ethyl alcohol, conducting concentrating, drying and smashing to obtain defatted enteromorpha powder, removing protein, polysaccharide and pigment, adding fucosyltransferase into a reaction system containing enteromorpha oligosaccharide, and conducting fucus glycosylation reaction to prepare fucus glycosylation enteromorpha oligosaccharide. It is proved that glycosylation enteromorpha oligosaccharide has strong restraining activity for alpha-glucosaccharase, remarkably increases the number of intestinal probiotics, and can be used for preparing medicines or health care products for preventing and treating diabetes and improving metabolic diseases of intestinal flora and the like.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and relates to a method for preparing glycosylated Enteromorpha oligosaccharides for lowering blood sugar and regulating intestinal flora. Background technique [0002] With the improvement of people's life, the population of hyperglycemia is expanding day by day, diabetes and blood circulation system diseases caused by hyperglycemia are more and more harmful to human health. Research data in recent years have shown that intestinal flora is closely related to type 2 diabetes. Bifidobacteria are the dominant genus in the human intestinal flora and one of the important signs of human health. The reduction of the number of bifidobacteria can lead to the dysbiosis of the whole intestinal flora and the imbalance of intestinal microecology, which leads to the occurrence of many metabolic diseases. Bifidobacteria can effectively improve glucose tolerance and glucose-induced insulin secretio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04A23L33/125A61K31/702A61P3/10A61P1/00A61P3/00
CPCA61K31/702C12P19/04C12P19/18
Inventor 赵超吴一晶刘斌李秋哲杨成凤陈玉青
Owner 清枫链食苏打饮品(吉林)有限公司
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