Preparation and application of organic polymer liquid phase integral chromatographic column of hydrophilic choline phosphoric acid matrix
A monolithic chromatographic column, choline phosphoric acid technology, applied in the field of chromatographic separation, can solve the problems of limited application optimization range, narrow solvent selection range, limited polarity of chromatographic column, etc., and achieve excellent hydrophilic performance, good acid and alkali resistance, The effect of high column efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] The functional monomer MBP, the cross-linking agent EDMA, the porogen (including isopropanol and tetrahydrofuran) and the initiator azobisisobutyronitrile AIBN were prepared into a reaction mixture, and after ultrasonic degassing, it was poured into the γ-MAPs pretreated In the quartz capillary, then seal both ends of the quartz capillary, and put it into a 60 °C water bath for 12 hours; after the reaction, connect the quartz capillary to a high-pressure pump, wash away unreacted substances, and obtain poly(MBP-co-EDMA) Aqueous phosphocholine-based organic polymer liquid monolithic column. Wherein the mass ratio of MBP and cross-linking agent is 57.2:42.8, the mass ratio of isopropanol and tetrahydrofuran in porogen is 35.5:64.5, the sum of MBP and cross-linking agent mass, and the mass ratio of porogen is 21.3: 78.7, the mass of the initiator is 1% of the sum of the mass of the MBP and the crosslinking agent. The schematic diagram of the preparation of the monolithic ...
Embodiment 2
[0034] The functional monomer MBP, the cross-linking agent EDMA, the porogen (isopropanol and tetrahydrofuran) and the initiator azobisisobutyronitrile AIBN were prepared into a reaction mixture, and after ultrasonic degassing, it was poured into the γ-MAPs pretreated In the quartz capillary, then seal both ends of the quartz capillary and put it in a 60°C water bath to react for 12 hours. After the reaction is completed, connect the quartz capillary to a high-pressure pump to wash away unreacted substances to obtain poly(MBP-co-EDMA) hydrophilic An organic polymer liquid phase monolithic column with a phosphocholine matrix. Wherein the mass ratio of MBP and cross-linking agent is 57.2:42.8, the mass ratio of isopropanol and tetrahydrofuran in porogen is 33.0:67.0, the sum of MBP and cross-linking agent mass, and the mass ratio of porogen is 21.3: 78.7, the mass of the initiator is 1% of the sum of the mass of the MBP and the crosslinking agent.
Embodiment 3
[0036] The functional monomer MBP, the cross-linking agent EDMA, the porogen (isopropanol and tetrahydrofuran) and the initiator azobisisobutyronitrile AIBN were prepared into a reaction mixture, and after ultrasonic degassing, it was poured into the γ-MAPs pretreated In the quartz capillary, then seal both ends of the quartz capillary and put it in a 60°C water bath to react for 12 hours. After the reaction is completed, connect the quartz capillary to a high-pressure pump to wash away unreacted substances to obtain poly(MBP-co-EDMA) hydrophilic An organic polymer liquid phase monolithic column with a phosphocholine matrix. Wherein the mass ratio of MBP and cross-linking agent is 57.2:42.8, the mass ratio of isopropanol and tetrahydrofuran in porogen is 38.0:62.0, the sum of MBP and cross-linking agent mass, and the mass ratio of porogen is 21.3: 78.7, the mass of the initiator is 1% of the sum of the mass of the MBP and the crosslinking agent.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More