Primer system for PCR identification on pig, bovine and sheep derived components of livestock and PCR identification method and kit

Abstract

The invention discloses a primer system for identifying components derived from pigs, cattle and sheep in livestock and poultry meat, a PCR identification method and a kit. Design specific primers according to the differential sites of animal mitochondrial DNA 16S rRNA genes, see sequence list SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6. The PCR identification method includes extracting the DNA of the sample to be tested and PCR amplification. A kit comprising SEQ ID NO.1‑6. The primers of the present invention only specifically amplify the target fragments of their respective species, and will not react with other species. The detection sensitivity is high, and the components derived from pigs, cattle, and sheep in livestock and poultry meat can be effectively identified. The method is rapid , sensitive and practical; if the primer system is combined with relevant reagents, it can be made into a kit, which provides the possibility for industrial production and application, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to a primer system, a PCR identification method and a kit for the pig, cattle, and sheep-derived PCR identification in livestock and poultry meat, and is suitable for the rapid detection and identification of the above-mentioned three kinds of animal meat. identification. Background technique [0002] The quality and safety of meat and meat products has always been a hot topic of concern to the whole society. With the widening price gap between pork, beef, and mutton in the market, some unscrupulous businesses or individuals use relatively cheap pork to sell it as beef and mutton products through various means of deep processing in order to pursue their own interests, seriously infringing on consumption. The legitimate rights and interests of consumers directly affect the health of consumers. In recent years, with the multiple exposures of "beef paste", fake beef, mutto...

AI Technical Summary

Problems solved by technology

[0004] In recent years, the design of species-specific primers and the establishment of PCR and real-time fluorescent PCR methods based on the differential sites of mitochondrial genomic DNA sequences have been reported abroad, but only a few domestic literatures have reported the use of PCR technology to identify meat sources. And most of its amplified sequences are based on microsatellite sequences in animal genomes. The sensitivity of the method is greatly affected by the extraction efficiency of genomic DNA, and the coverage of the species involved is small, and the detection range is narrow. The detection of bovine-derived, sheep-derived or porcine-derived components in this product still needs further verification

[0005] At present, there is no relevant report on the establishment of a method for the identification of pig, cattle and sheep-derived components in livestock and poultry meat based on the mitochondrial DNA16SrRNA gene

Images