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Preparation method and application of medical grade fish skin collagens

A fish skin and collagen technology, applied in the field of biomedical materials, can solve the problems of not taking virus inactivation, long cycle, low protein purity, etc., and achieve the effect of reducing the risk of residual virus, reducing difficulty, and short cycle

Active Publication Date: 2016-07-13
烟台蓝创生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current research on the extraction of macromolecular collagen from fish skin is mostly in the stage of laboratory experiments. The production process has many shortcomings such as high energy consumption, long cycle, low protein purity, many impurities, and no virus inactivation measures. Meeting the production quality requirements of biomedical materials also greatly limits the application of fish skin collagen as a raw material in the development of medical products

Method used

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  • Preparation method and application of medical grade fish skin collagens
  • Preparation method and application of medical grade fish skin collagens
  • Preparation method and application of medical grade fish skin collagens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of extraction method of tilapia skin collagen

[0038] 1. Pretreatment of fish skin

[0039] Take 100g of fresh tilapia skin, remove the scales, residual meat and fat, and cut it into an area smaller than 0.5cm with a slicer 2 Soak the small pieces with 1% NaCl at 15°C for 2 hours, the ratio of solid to liquid is 1:10 (w / v), repeat once, and drain;

[0040] 2. Wash the fish skin

[0041] 1. Degreasing

[0042] Add 0.1% Triton×100 (w / w) solution to the fish skin, the ratio of material to liquid is 1:10 (w / v), shake at 15°C and 100rpm for 12 hours, drain the water, rinse 3 times with pure water, and drain ;

[0043] 2. Remove impurities

[0044] Add 0.05M NaOH solution to the fish skin with a solid-liquid ratio of 1:10 (w / v), shake at 15°C and 200rpm for 6 hours, and drain; then add a 1% NaCl solution to the fish skin with a solid-liquid ratio of 1:10 (w / v), 8°C, shake at 100rpm for 12 hours, change the liquid every 4 hours, drain the water, rins...

Embodiment 2

[0059] Embodiment 2 A kind of extraction method of cod skin collagen

[0060] 1. Pretreatment of fish skin

[0061] Take 100g of fresh cod skin, remove the scales, residual meat and fat, and cut it into an area smaller than 0.5cm with a slicer 2 Soak the small pieces with 5% NaCl at 10°C for 0.5 hours, the ratio of solid to liquid is 1:30 (w / v), repeat once, and drain.

[0062] 2. Wash the fish skin

[0063] 1. Degreasing

[0064] Add 1% Tween 80 (w / w) solution to the fish skin, the ratio of material to liquid is 1:30 (w / v), shake at 10°C and 250rpm for 6 hours, drain the water, rinse 3 times with pure water, and drain ;

[0065] 2. Remove impurities

[0066] Add 0.2M KOH solution to the fish skin, the ratio of solid to liquid is 1:30 (w / v), shake at 10°C, 250rpm for 3 hours, drain; then add a solution containing 5% NaCl to the skin, the ratio of solid to liquid is 1:30 (w / v), 10°C, shake at 250rpm for 6 hours, change the liquid every 2 hours, drain the water, then rinse...

Embodiment 3

[0081] Embodiment 3 A kind of extraction method of shark skin collagen

[0082] 1. Pretreatment of fish skin

[0083] Take 100g of fresh shark skin, remove the scales, residual meat and fat, and cut it into an area smaller than 0.5cm with a slicer 2 Soak the small pieces in 3% KCl at 20°C for 1 hour, the ratio of solid to liquid is 1:20 (w / v), repeat once, and drain.

[0084] 2. Wash the fish skin

[0085] 1. Degreasing

[0086] Add 0.3% Tween 20 solution to the fish skin, the ratio of solid to liquid is 1:20 (w / v), shake at 20°C and 175rpm for 9 hours, drain the water, rinse with pure water 3 times, and drain;

[0087] 2. Remove impurities

[0088] Add 0.1MCa(OH) to the fish skin 2 Solution, solid-liquid ratio 1:20 (w / v), 20°C, 175rpm shake for 4.5 hours, drain; then add 3% KCl solution to fish skin, solid-liquid ratio 1:20 (w / v), 20 ℃, shake at 175rpm for 9 hours, change the liquid every 3 hours, drain the water, then rinse with pure water for 3 times, and drain;

[0...

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PUM

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Abstract

The invention discloses a method for extracting macromolecular medical collagens from fish skins, and an application of the fish skin collagens as a tissue restoration scaffold material. The method comprises the following steps: 1, preprocessing fish skins; 2, cleaning the fish skins through various technologies; 3, extracting the collagens through an acid technology; 4, processing the collagens through an immobilized enzyme technology; and 5, purifying the collagens. The collagen preparation method has the advantages of low energy consumption, short period, simple operation, and suitableness for industrial large-scale production, and the prepared collagens have the advantages of high purity, low immunogenicity and good biocompatibility, and are suitable for being processed to develop medical collagen products. The invention also discloses an application of the fish skin collagens in the production of a scaffold material and an application of the scaffold material in wound restoration.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a method for extracting macromolecular collagen that can be used as a biomedical material from fish skin, and the application of fish skin collagen as a tissue repair support material. Background technique [0002] Collagen is the main component of the extracellular matrix and has become one of the main raw materials for biomedical materials because of its good biocompatibility. In recent years, with the expansion of the application field of collagen-based biomaterials, the demand for its products has been increasing. But at the same time, collagen products mainly derived from mammals such as cattle, sheep, and pigs have many problems such as limited raw material sources, high production costs, and potential dangers of pathogen transmission such as mad cow disease and foot-and-mouth disease. Therefore, the development of new collagen resources and the research on related proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30A61L27/24
Inventor 李东升宫世周傅风华张淑敏杨小平
Owner 烟台蓝创生物技术有限公司
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