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A method for preparing porous material with silkworm silk fibroin and carboxymethyl chitosan

A technology of carboxymethyl chitosan and porous materials, applied in medical science, prosthesis, etc., can solve problems such as poor water absorption, unsatisfactory uniformity of pore size, rough shape and surface, etc., and achieve soft texture and strong plasticity , smooth surface effect

Inactive Publication Date: 2019-09-10
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After further research, it was found that the preparation method disclosed in this document still has the following problems, mainly because the prepared scaffold material ① rough surface; ② unsatisfactory uniformity of pore size; ③ poor water absorption, etc.

Method used

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  • A method for preparing porous material with silkworm silk fibroin and carboxymethyl chitosan
  • A method for preparing porous material with silkworm silk fibroin and carboxymethyl chitosan
  • A method for preparing porous material with silkworm silk fibroin and carboxymethyl chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing a porous biological material, carried out according to the following steps:

[0036] Table 1 The ratio of silk fibroin / carboxymethyl chitosan and the ratio of glycerol and glutaraldehyde

[0037]

[0038] 1. Preparation of regenerated silk fibroin solution

[0039] at 0.5% Na 2 CO 3 Degumming in solution to obtain fibrous silk fibroin, and then in CaCl 2 、CH 3 CH 2 OH, H 2 O dissolved silk fibroin in a ternary solvent with a molar mass ratio of 1:2:8, and obtained a regenerated silk fibroin solution after dialysis. Specific steps are as follows:

[0040] (1) Degumming

[0041] Weigh a certain amount of silkworm cocoons, cut the cocoon shells into 1 square centimeter pieces after removing the silkworm chrysalis, add boiling 0.5% Na 2 CO 3 The solution (bath ratio is 1:50) was boiled for 30 minutes. Take out the cocoons and wash them repeatedly with deionized water until there is no greasy feeling. Repeat 2 times, and then detect with...

Embodiment 2

[0088] A method for preparing a porous biological material, carried out according to the following steps:

[0089] Table 2 silk fibroin / carboxymethyl chitosan ratio and the ratio of glycerol and glutaraldehyde

[0090]

[0091] 1. Preparation of regenerated silk fibroin solution

[0092] at 0.5% Na 2 CO 3 Degumming in solution to obtain fibrous silk fibroin, and then in CaCl 2 、CH 3 CH 2 OH, H 2 O dissolved silk fibroin in a ternary solvent with a molar mass ratio of 1:2:8, and obtained a regenerated silk fibroin solution after dialysis. Specific steps are as follows:

[0093] (1) Degumming

[0094] Weigh a certain amount of silkworm cocoons, cut the cocoon shells into 1 square centimeter pieces after removing the silkworm chrysalis, add boiling 0.5% Na 2 CO3 The solution (bath ratio is 1:50) was boiled for 30 minutes. Take out the cocoons and wash them repeatedly with deionized water until there is no greasy feeling. Repeat 2 times, and then detect with bitter y...

Embodiment 3

[0107] A method for preparing a porous biological material, carried out according to the following steps:

[0108] Table 3 silk fibroin / carboxymethyl chitosan ratio and the ratio of glycerol and glutaraldehyde

[0109]

[0110] 1. Preparation of regenerated silk fibroin solution

[0111] at 0.5% Na 2 CO 3 Degumming in solution to obtain fibrous silk fibroin, and then in CaCl 2 、CH 3 CH 2 OH, H 2 O dissolved silk fibroin in a ternary solvent with a molar mass ratio of 1:2:8, and obtained a regenerated silk fibroin solution after dialysis. Specific steps are as follows:

[0112] (1) Degumming

[0113] Weigh a certain amount of silkworm cocoons, cut the cocoon shells into 1 square centimeter pieces after removing the silkworm chrysalis, add boiling 0.5% Na 2 CO 3 The solution (bath ratio is 1:50) was boiled for 30 minutes. Take out the cocoons and wash them repeatedly with deionized water until there is no greasy feeling. Repeat 2 times, and then detect with bitter...

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Abstract

The invention provides a method for preparing a porous material. The method is characterized in that silk fibroin and carboxymethyl chitosan serve as raw materials; the method includes the steps of preparing a silk fibroin solution, preparing a carboxymethyl chitosan solution and preparing a silk fibroin and carboxymethyl chitosan composite material; preparation of the silk fibroin and carboxymethyl chitosan composite material is formed in the mode that cross-linking glutaraldehyde, plasticizer glycerin, fibroin and carboxymethyl chitosan are mixed and pre-cooled, and freezing and drying are conducted; the mixture is placed for 6 h at the temperature of -20 DEG C for pre-cooling and then placed for 12 h at the temperature of -80 DEG C. By means of the method, time is saved, the surface of the obtained material is smooth, texture is soft, the material is fluffy and porous and high in plasticity, and the shape of the material can be changed according to requirements so that the material can be widely applied to biomedicine. Besides, the aperture of the prepared material is more uniform and about 100 microns, water absorption is better, and the material has the antibacterial and anti-infection functions and is better in biocompatibility with organisms.

Description

technical field [0001] The invention relates to the technical fields of biological tissue engineering and biological materials, and discloses a novel porous material and a preparation method thereof, specifically a method for preparing the porous material by using regenerated silk fibroin and carboxymethyl chitosan. Background technique [0002] Silk fibroin is a natural protein with a wide range of sources and good biocompatibility. Its amino acid composition has health care effects on the human body and is an ideal raw material for the development of medical polymer materials. However, pure silk fibroin film has poor mechanical properties and is easily soluble in water, which greatly limits its application. Carboxymethyl chitosan is a macromolecule with high biological safety. It has a large number of hydroxyl groups and carboxyl groups, which can form intermolecular hydrogen bonds with amino groups and hydroxyl groups in silk fibroin molecules to prevent the formation of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/50
Inventor 赵诏张岩朱勇徐水
Owner SOUTHWEST UNIV
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