Bacillus subtilis for degrading edible mushroom dreg cellulose and application of bacillus subtilis

A technology of Bacillus subtilis and edible fungus, which is applied in the field of microorganisms and can solve the problems of slow degradation of cellulose

Inactive Publication Date: 2016-07-27
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of slow degradation of cellulose in the fermentation process of fungus residues, the present invention provides a Bacillus subtilis that degrades cellulose from edible fungus residues and its application

Method used

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  • Bacillus subtilis for degrading edible mushroom dreg cellulose and application of bacillus subtilis
  • Bacillus subtilis for degrading edible mushroom dreg cellulose and application of bacillus subtilis
  • Bacillus subtilis for degrading edible mushroom dreg cellulose and application of bacillus subtilis

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: Isolation, cultivation, identification and degradation characteristics of Bacillus subtilis (Bacillus subtilis) NFB7 (preservation number: CGMCCNO.11927)

[0036] 1. Materials and methods

[0037] 1.1 Test materials

[0038] Edible mushroom residues are collected from Pleurotus eryngii mushroom residues that are naturally stacked in Tongzhou Yongle Store, Beijing.

[0039] 1.2 Culture medium type

[0040] ① Enrichment medium formula: peptone 10.0g, K 2 HPO 4 1.0g, Na 2 CO 3 5.0g, MgSO 4 ·7H 2 O0.1g, FeSO 4 ·7H 2 O0.015g, MnSO 4 0.05g, yeast extract 10g, distilled water 1000mL, pH6.0.

[0041] ② LB solid medium formula: yeast extract 5g, NaCl 10g, peptone 10g, water 1000mL, agar 18g, pH 7.0.

[0042] ③Carboxymethylcellulose sodium medium (CMC-Na medium) formula: carboxymethylcellulose sodium (CMC-Na) 15.0g, yeast 1.0g, NH 4 PO 3 1.0g, MgSO 4 ·7H 2 O0.5g, K 2 HPO 4 1.0g, 18g agar, 1000mL distilled water.

[0043] ④ Liquid seed medium formu...

Embodiment 2

[0091] The composting effect of embodiment 2 bacillus subtilis (Bacillus subtilis) NFB7 bacterial strain to mushroom slag cultivation Agaricus bisporus

[0092] 1 Materials and methods

[0093] 1.1 Experimental materials

[0094] Bacterial agent: (NFB7+BCB4+BCB2) mixed bacterial agent, which is composed of NFB7 (preservation number: CGMCCNO.11927), BCB4 (preservation number: CGMCCNO.11141) and BCB2 (preservation number: CGMCCNO.11140).

[0095] The preservation number is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BCB4 with the preservation number CGMCCNO.11141, which was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on July 21, 2015, address: Beichen West Road 1, Chaoyang District, Beijing No. 3 Courtyard, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101.

[0096] The preservation number is Bacillus tequilensis BCB2 with the preservation number CGMCCNO.11140, which was preserved in...

Embodiment 3

[0124] The fertility effect of embodiment 3 bacillus subtilis (Bacillus subtilis) NFB7 bacterial strain to slag compost

[0125] 1 Experimental materials and methods

[0126] 1.1 Experimental materials

[0127] Collect the discarded fungus bags after Bailing mushroom and Lentinus edodes are cultivated, and use the bag breaking machine to remove the bags and crush them for later use. Add 0.23 tons of dry cow dung and 5 L of (NFB7+BCB4+BCB2) mixed bacteria agent per ton of fungus residue (wet).

[0128] 1.2 Experimental method

[0129] The ratio of the slag of Bailing mushroom to that of shiitake mushroom is roughly 1:1, and the moisture content of the stock is preferably 65%-70%. When the temperature in the pile rises to 60°C and is maintained for 24 hours, the second turning is carried out. After that, turn over the pile every 2-3 days until the temperature no longer rises. Be sure to turn the pile evenly and thoroughly so that it can fully rot. If the material is found t...

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Abstract

The invention relates to bacillus subtilis NFB7 for degrading edible mushroom dreg cellulose. The accession number of bacillus subtilis is CGMCC NO. 11927. The invention also provides a complex microbial inoculant, which comprises the following active ingredients: bacillus subtilis NFB7 with accession number of CGMCC NO. 11927, bacillus amyloliquefaciens BCB4 with accession number of CGMCC NO. 11141, and bacillus tequilensis BCB2 with accession number of CGMCC NO. 11140. After the complex microbial inoculants is added to compost, the contents of available nitrogen, available phosphorus and available potassium in the compost are improved; by using the produced compost for planting, the yield and thousand seed weight are respectively increased by 8.14% and 5.57% compared with the conventional fertilizer way.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus subtilis for degrading edible fungus residue cellulose and an application thereof. Background technique [0002] Mushroom residue is the culture medium waste left over from harvesting products during the cultivation of edible fungi. Along with the development of edible fungus industry, a large amount of fungus residues are produced thereupon every year. Especially in some large production bases, tons of fungus residues need to be processed every day. A large number of mushroom residues and waste sticks are piled up or discarded at will, which not only causes environmental pollution and waste of resources, but also affects the production of edible fungi, bringing a certain burden to enterprises and producers. Mushroom residue composting is the main way to effectively treat edible fungus residue. The composted mushroom residue can be used as raw material for t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F15/00C12R1/125C12R1/07
CPCC05B1/02C05F11/00C05F11/08C12N1/20C12N1/205C12R2001/125C05F3/00
Inventor 邹亚杰胡清秀刘晓梅
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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