Gene transformation method for summer black grapes

Abstract

The invention discloses a gene transformation method for summer black grapes. The method comprises the steps of material selection from tissue culture seedling leaves, leaf stalks and stem fragments, infection, co-culture, screening culture and rooting culture. Material selection is optimized, growth portions and growth time of explant leaves are specifically regulated, and the phenomenon that the working efficiency is low due to blindness of material selection is avoided; meanwhile, the portions, including grape leaf stalks and stem fragments, except the leaves, of the explants are selected, better operation can be performed by using the leaf stalks and the stem fragments, the gene transformation efficiency can be improved, and the transformation efficiency can reach 20% or above; the problem of pollution of agrobacterium and infectious microbes in an agrobacterium-mediated method is solved; the problem that the grape conversion efficiency is low is solved; the problem that grape transgene transgenosis, transgenic test and farm transplanting period is long is solved, and the period is shortened; the problems that the transgenic test workload is large and workloads are large are solved, the detection method is simplified, and the detection speed is increased.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a grape gene transformation method. Background technique [0002] Grape is a crop with high economic value and rich nutritional value. In recent years, grape industry has developed rapidly in many areas of our country and has become an important part of agricultural industrialization in this area. In recent years, Chinese scientific researchers have also invested a lot of energy in the research of grapes. Especially with the rapid development of molecular biology and genetic engineering, the research focus has turned to the deep mining of excellent genes in grape germplasm resources, and the molecular markers and Identification and determination of gene expression sequences. At the same time, research results on genetic improvement such as stress resistance, storage and transportation resistance, and flavor quality have been reported one after another, and grape bree...

AI Technical Summary

Problems solved by technology

Although the genetic transformation technology route of grapes is mature, the transformation efficiency is very low, and too few transgenic plants are obtained, which brings obstacles to the later identification of transgenic plants

The operation process of the traditional Agrobacterium-mediated method is cumbersome, and many problems are prone to occur during the transgenic process, such as the selection of suitable vectors, the selection of suitable strains, the contamination of miscellaneous bacteria, the inability of Agrobacterium to be inhibited, and the concentration of bacteria solution is too high or too high. Low results in unsuccessful transgenics, control of the duration of infection, etc. These problems will affect the efficiency and speed of grape transgenics. The transgenic efficiency of grape plants obtained by traditional methods is less than 1%.

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