Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting bismuth ions by utilizing rhodamine-type fluorescent probe

A technology of fluorescent probes and bismuth ions, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of few aluminum ions, no indication of the detection range and detection limit of bismuth ions, etc., and achieve a good choice Sexuality, low cost, good use effect

Inactive Publication Date: 2016-08-17
DONGHUA UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After reviewing Chinese patents, it was found that there are very few patents using fluorescence spectroscopy to detect aluminum ions, and no patents indicate the relationship between the probe and the concentration of bismuth ions, and there is no indication of the detection range and detection limit of bismuth ions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting bismuth ions by utilizing rhodamine-type fluorescent probe
  • Method for detecting bismuth ions by utilizing rhodamine-type fluorescent probe
  • Method for detecting bismuth ions by utilizing rhodamine-type fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Synthesis of rhodamine fluorescent probe

[0039] (1) Synthesis of rhodamine B hydrazide

[0040] Take a 100 mL three-necked flask, weigh 1.200 g (2.5 mmol) of rhodamine B and dissolve it in 30 mL of absolute ethanol, stir vigorously at room temperature and slowly add 1 mL (19.8 mmol excess) of 98% hydrazine hydrate into the inside. Heat at 78°C under reflux for 2 hours until the solution turns from dark purple to clear yellow. After the reaction is completed, it is cooled to room temperature, and the solvent and excess hydrazine hydrate are evaporated using a rotary evaporator to obtain a pale yellow crude product of rhodamine B hydrazide. Take 50mL of the newly prepared 1M HCl and add it to the crude product. The product dissolves in a pink solution. Under stirring, slowly add 1M NaOH to the solution to adjust the pH to between 9-10. When NaOH was added dropwise to the mixed solution, a pale pink flocculent precipitate began to precipitate when the pH of the ...

Embodiment 2

[0052] Take the prepared bismuth nitrate pentahydrate solution (6×10 -7 M) As the bismuth ion test solution, the method for detecting bismuth ions in the rhodamine-based fluorescent probe synthesized in Example 1 is as follows:

[0053] Step 1: Dissolve the rhodamine-based fluorescent probe synthesized in Example 1 in the solvent acetonitrile, and use the solvent acetonitrile to dilute the volume in a 100 mL volumetric flask to obtain a concentration of 1.0×10 -3 M probe stock solution, pipette the probe stock solution into a 100mL volumetric flask, use the solvent acetonitrile to make a constant volume in a 100mL volumetric flask to obtain a concentration of 1.0×10 -4 M probe solution;

[0054] Step 2: Dissolve bismuth nitrate (containing pentahydrate crystal water) in the solvent deionized water, and use the solvent deionized water to make the volume in a 100mL volumetric flask to obtain a concentration of 1.0×10 -2 M bismuth ion stock solution; pipet the bismuth ion stock solution...

Embodiment 3

[0058] Bismuth nitrate (containing pentahydrate crystal water) was added to acetonitrile to obtain a bismuth ion solution with a concentration of 100 nM. The fluorescent probe synthesized in Example 1 was dissolved in acetonitrile to obtain 10 -5 M concentration of fluorescent probe solution, add 15ml fluorescent probe (10 -5 M) Add 10ml of bismuth ion solution to the solution and test the fluorescence intensity after standing for 5 minutes. The excited state absorption spectrum and fluorescence emission spectrum of the complex of probe and bismuth ion in acetonitrile solution.

[0059] figure 1 Shown using 557nm light for excitation (probe-Bi 3+ ) Complex, the emission peak is located at 580nm, the ligand fluorescent probe (10 -5 M) Measure the fluorescence intensity at an excitation wavelength of 557nm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for detecting bismuth ions by utilizing a rhodamine-type fluorescent probe. The method for detecting the bismuth ions by utilizing the rhodamine-type fluorescent probe is characterized by comprising the following steps of preparing a probe solution by utilizing the rhodamine-type fluorescent probe, adding the probe solution into to-be-detected bismuth-ion liquid, fixing a volume by utilizing a solvent, enabling an obtained mixture to stand for a period of time, detecting fluorescence intensity, and determining the content of the bismuth ions in the to-be-detected bismuth-ion liquid according to a relation between the fluorescence intensity and the concentration of the bismuth ions. A functional active dye in the method for detecting the bismuth ions by utilizing the rhodamine-type fluorescent probe has quite good selectivity for the bismuth ions, and is not only convenient but also has a better usage effect in sewage treatment application.

Description

Technical field [0001] The invention belongs to the field of functional reactive dyes and their preparation, and particularly relates to a method for detecting bismuth ions by using a reactive dye fluorescent probe. Background technique [0002] The metal ions in nature are closely related to the life sciences, environmental sciences, medicine and other fields on which humans depend. Bismuth, as a chemically stable element with relatively large atomic mass, is mostly in the form of free metals and minerals in nature exist. It is often used to make easily soluble alloys in industrial production. As a "green metal", compounded bismuth is also widely used in the treatment of gastrointestinal diseases and skin injuries in the medical field. At the same time, studies have found that long-term use of bismuth-containing drugs will cause large amounts of metal elements to be deposited in the brain and kidneys, which will trigger various related pathological changes, such as uremia, mem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 徐洪耀魏刚光善仪张月华
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com