Rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum

A technology for hybrid orchid and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long cycle, low reproduction coefficient, and no protocorm obtained, and achieves convenient material collection, simplified cultivation process, shortened The effect of incubation time

Inactive Publication Date: 2016-08-24
SHANGHAI ACAD OF AGRI SCI +2
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Problems solved by technology

[0006] Cao Shoujin carried out tissue culture research using the tip, stem segment and leaf of the tiger head orchid as explants, and the results showed that: suitable protocorm induction medium: KC+5mg / L 6-BA+0.5 (or 1.0)mg / L NAA+0.5~1% sucrose; the protocorm induction rate of the shoot tip is 38~75%; the protocorm induction rate of the stem segment is 48~78%; the protocorm induction rate of the leaf is 5~16%; proliferation medium: KC+5mg / L BA+0.1 (or 0.5) mg / L NAA+0.2 peptone; the fresh weight increase rate of protocorms is 50-58% (Cao Shoujin, Northern Horticulture, 2007 (3): 167-169), but, This induction medium is used in the tissue culture of the hybrid orchid seedlings of Molan and tiger head orchid, and no protocorms are obtained.
[0007] "Red Beauty" is a hybrid variety of Molan Hongyingji and Tiger Head Orchid. It has a beautiful plant shape, a long flowering period, and a combination of large flowers, flower fragrance, and beautiful flower color. At present, its propagation method is mainly conventional branch propagation. Only 1 to 3 times the multiplication rate can be obtained every year, the reproduction coefficient is low, and the cycle is long

Method used

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  • Rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum
  • Rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum
  • Rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum

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Embodiment 1

[0038] A rapid propagation method for the hybrid orchid of Molan and Sagittarius, comprising the following steps:

[0039] 1) Induction culture

[0040]Take the aseptic seedling of "Red Beauty", the hybrid offspring of Molan and Hutoulan, cut off the roots and top leaves, take 0.6 cm of basal tissue, peel off the outer 1-2 layers of leaf sheaths, and inoculate it in the induction medium; carry out light culture, Cultured for 50d to obtain hybrid orchid protocorm (see figure 1 ), the induction rate of protocorm reached 83.3%;

[0041] Among them, the induction medium is: MS+6-BA 5.0mg / L+KT 1.0mg / L+NAA1.0mg / L+PIC 0.3mg / L+hydrolyzed casein 1.0mg / L+activated carbon 0.1g / L+sucrose 30g / L+ Agar powder 6.0g / L, pH=5.6;

[0042] 2) Proliferation culture and differentiation

[0043] Take the hybrid blue protocorm obtained in step 1), cut it, and inoculate it in the protocorm proliferation and differentiation medium. The formed protocorm gradually differentiates into numerous adventi...

Embodiment 2

[0051] A rapid propagation method for the hybrid orchid of Molan and Sagittarius, comprising the following steps:

[0052] 1) Induction culture

[0053] Take the aseptic seedlings of "Red Beauty", the hybrid offspring of Molan and Hutoulan, cut off the roots and top leaves, take 0.8 cm of the basal tissue, peel off 1-2 layers of leaf sheaths, and inoculate them in the induction medium; carry out light culture, After culturing for 53 days, the hybrid orchid protocorm was obtained, and the induction rate of protocorm reached 75.0%;

[0054] Among them, the induction medium is: MS+6-BA 4.0mg / L+KT 1.5mg / L+NAA1.0mg / L+PIC 0.2mg / L+hydrolyzed casein 1.0mg / L+activated carbon 0.4g / L+sucrose 30g / L+ Agar powder 6.0g / L, pH=5.6;

[0055] 2) Proliferation culture and differentiation

[0056] Take the hybrid blue protocorm obtained in step 1), cut it, and inoculate it in the protocorm proliferation and differentiation medium. The formed protocorm gradually differentiates into a large numb...

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Abstract

The invention discloses a rapid propagation method for hybrid cymbidium seedlings of cymbidium sinense and cymbidium hookerianum. Hybrid cymbidium aseptic seedling base tissue of the cymbidium sinense and the cymbidium hookerianum is used as a raw material, induction culture is performed in an induction culture medium containing various plant growth regulators and additives, protocorms are obtained and subjected to proliferation and differentiation culture, and then seedling strengthening, rooting and transplantation are performed. Steps of proliferation and differentiation of the protocorms are combined, the culture procedure is simple, the material cost for preparation of the culture medium is greatly reduced, the labor cost for preparation of the culture medium and inoculation is also reduced, the inductivity of the protocorms is high, the quantity of the protocorms is large, the proliferation coefficient is large, and the method has multiple advantages of low production cost, high seedling quality, short culture period, avoidance of variation and the like.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of orchids, and in particular relates to a rapid propagation method for hybrid orchid seedlings of Molan and Sagittarius. Background technique [0002] Cymbidium sinense is a terrestrial orchid perennial herb of the Orchidaceae (Orchidaceae) genus (Cymbidium) and is one of the five traditional national orchids. , has high ornamental value and economic value. Because it mostly blooms before and after the Spring Festival, it is also known as the old orchid. Its traditional ramet propagation method has a very low reproduction coefficient, and it can only multiply by 1 to 3 times a year. [0003] Cymbidium hookerianum is an epiphytic perennial herb of the genus Orchidaceae. Native to the southwest of China, it is mainly distributed in Guizhou, Tibet, Yunnan, Sichuan and other provinces and regions, and is introduced and cultivated in many countries around the world. At present, most of the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 朱建军殷丽青张春英李秀芬孙翊范晓芬张治忠
Owner SHANGHAI ACAD OF AGRI SCI
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