Lactobacillus buchneri inoculant as well as preparation method and application thereof

A technology of Lactobacillus brucei and microbial agent, which is applied in the field of Lactobacillus brunii bacterial agent and its preparation, can solve the problems of increasing milk production and adverse effects of aerobic stability, so as to improve performance and improve aerobic stability , the effect of a wide range of application prospects

Inactive Publication Date: 2016-09-21
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This compound lactic acid bacteria preparation can improve the preservation performance of corn silage and increase milk production and milk fat percentage, but whether it has an effect on the content of aflatoxin in silage is unknown
Patent C

Method used

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  • Lactobacillus buchneri inoculant as well as preparation method and application thereof
  • Lactobacillus buchneri inoculant as well as preparation method and application thereof
  • Lactobacillus buchneri inoculant as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Preparation of Lactobacillus brucei inocula

[0026] (1) Expansion culture of bacteria: inoculate Lactobacillus brucei in the MRS liquid medium, culture for 48 hours at 37°C, and insert the obtained bacterial liquid into the new MRS liquid medium at a ratio of 3%, and expand the culture for 24 hours , Obtain Lactobacillus brucei culture solution;

[0027] (2) Bacterial cell collection: Centrifuge the obtained Lactobacillus brucei culture solution at 8000 rpm for 15 minutes, and wash the obtained Lactobacillus brucei cell with normal saline three times, and finally obtain the cleaned Brinell milk Bacillus cells;

[0028] (3) Preparation of bacterial agent: Add 10% skimmed milk powder to the cleaned Lactobacillus brucei cells, mix well, freeze at -80℃ for 4h; then at a temperature of -50 and a vacuum of ℃ Under the condition of 10Pa, freeze-dry for 36h to obtain Lactobacillus brucei inoculum.

Embodiment 2

[0029] Example 2: Preparation of whole-plant corn silage

[0030] On the 7th day after half of Jingke 516 silking, 2.1ml of toxin-producing Aspergillus flavus was inoculated on one side of the corn ear, and the number of toxin-producing Aspergillus flavus was 1×10 7 , Purchased from China Collection of Common Microorganisms.

[0031] Cut Jingke 516, which has a moisture content of close to 65% in the wax maturity period, to ensure that the raw materials are clean and free of mold and deterioration, and then cut short to 2-3cm to obtain the silage raw materials; then the diluted Lactobacillus brucei inocula Spray on the silage raw materials and mix evenly, so that each gram of silage raw materials contains 1×10 5 CFU Lactobacillus brucei; finally fill and compact the silage raw materials, pay attention to the corners and surroundings of the silage pit (bucket) during the compaction process to ensure sufficient compaction, and the compaction process is completed within the day of mowi...

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Abstract

The invention discloses a lactobacillus buchneri inoculant belonging to the technical field of quality safety of silage as well as a preparation method and an application of the lactobacillus buchneri inoculant. The lactobacillus buchneri inoculant is prepared from lactobacillus buchneri and dried skim milk, wherein the content of the lactobacillus buchneri in the lactobacillus buchneri inoculant is 1*10<10>-5*10<10> CFU/g. Lactobacillus buchneri thalli and a solution containing the dried skim milk are mixed and freeze-dried into powder under a vacuum freezing condition, and the inoculant is prepared. When applied to preparation of whole corn silage, the inoculant can improve the aerobic stability of the whole corn silage remarkably, inhibits mould proliferation in an aerobic spoilage process, reduces the content of aflatoxin B1 and improves the performance of the whole corn silage. The preparation method of the lactobacillus buchneri inoculant is simple and feasible, and the lactobacillus buchneri inoculant has wide application prospect in the technical field of quality safety of the silage.

Description

Technical field [0001] The invention belongs to the technical field of silage quality safety, and specifically relates to a Lactobacillus brucei inoculum and a preparation method and application thereof. Background technique [0002] In most ruminant production systems, 40-90% of livestock feed requirements come from roughage, and whole-plant corn silage is one of the main sources of roughage for livestock, especially high-yielding dairy cows. Whole-plant corn silage contains high soluble sugar and low buffer energy value, and it is easy to ferment by lactic acid bacteria. However, in the production process, many factors will cause the whole corn silage to come into contact with oxygen, resulting in aerobic deterioration: such as the delay of the harvesting period, most of the silage raw materials encounter early frost; the raw materials are cut short in the process of shortening. Too long; the filling density is reduced, and no heavy objects are used to compact it after filling...

Claims

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Application Information

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IPC IPC(8): A23K30/18A23K10/12A23K10/18C12N1/20C12R1/225
CPCC12N1/20
Inventor 玉柱田吉鹏李旭娇娜日苏
Owner CHINA AGRI UNIV
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