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Pachymaran polylactic acid nanoemulsion and preparing method and application thereof

A technology of Poria cocos polysaccharide and polylactic acid is applied in the field of medicine to achieve the effects of improving immune-enhancing activity, high encapsulation rate and strong sustained-release effect.

Active Publication Date: 2016-10-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a polylactic acid nanoemulsion of polysaccharides to solve the various limitations in the clinical application of the prior art

Method used

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  • Pachymaran polylactic acid nanoemulsion and preparing method and application thereof
  • Pachymaran polylactic acid nanoemulsion and preparing method and application thereof
  • Pachymaran polylactic acid nanoemulsion and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of Poria cocos polylactic acid nanoemulsion

[0039] Accurately weigh 10ml of acetone and 300mg of polylactic acid in a 10ml EP tube, and use a magnetic stirrer to form an oil phase; accurately weigh 15mg of pachyphyllin, dissolve it in 1ml of deionized water to form a water phase. While stirring the oil phase, add the water phase to the oil phase drop by drop until a stable W / O type colostrum is formed; in addition, accurately weigh 360mg of F68 and dissolve it in 110ml of deionized water, while stirring the F68 solution, add the colostrum Use a pipette gun to slowly inject under the liquid surface, and stir magnetically for 1.5 hours to form a stable W / O / W type double emulsion; remove the organic solvent by evaporating under reduced pressure at 55°C and 100 rpm on a rotary evaporator; use 3500 rpm for 8 minutes to remove the organic solvent Collect the supernatant from the large precipitate to obtain the polylactic acid nanoemulsion coated with p...

Embodiment 2

[0049] Example 2 Characterization of Poria cocos polylactic acid nanoemulsion

[0050] (1) Particle size potential detection and transmission electron microscope observation

[0051] The polysaccharide polylactic acid nanoemulsion prepared in Example 1 and the blank polylactic acid nanoemulsion were respectively taken, and the particle size and potential of the two types of nanoparticles were analyzed using a Malvern particle size analyzer. The results are shown in Table 3.

[0052] The surface morphology was analyzed by transmission electron microscopy, the results are shown in Figure 1A and Figure 1B .

[0053] table 3

[0054]

[0055]

[0056] It can be seen from the table that there is not much difference in size between polysaccharide polylactic acid nanoemulsion and blank polylactic acid nanoemulsion. At about 245nm, the PDI value is small, indicating that the particle size dispersion is relatively concentrated, and the potential is negative.

[0057] It can ...

Embodiment 3

[0061] Example 3 Comparison of Poria polysaccharide polylactic acid nanoemulsion in vitro immune enhancement activity

[0062] Taking the polylactic acid nanoemulsion (PHYP) prepared in Example 1 as the research object, and the blank polylactic acid nanoemulsion (PLA) prepared in Example 1 as a contrast, explore its in vitro effect on Effects on proliferation of mouse lymphocytes.

[0063] (1) Preparation of spleen lymphocytes

[0064] Mice were sacrificed by cervical dislocation and soaked in 75% ethanol for 3-5 minutes. In the ultra-clean bench, place the mouse in a plate with the left side facing up, open the skin and abdominal cavity at the last rib, carefully remove the spleen, and move the spleen into a 200-mesh steel sieve. Break the spleen with scissors and tweezers, add a little PBS, grind the spleen gently with a glass syringe sleeve, add PBS while grinding (add about 5 mL), and let the cell suspension flow into the plate. Mix the cell suspension evenly and put it...

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Abstract

The invention discloses pachymaran polylactic acid nanoemulsion. The nanoemulsion is prepared from raw materials including acetone, polylactic acid, pachymaran and propylene glycol block polyether F68 through a multiple emulsion method. The invention further discloses a preparing method of the pachymaran polylactic acid nanoemulsion and application to preparation of an immunological enhancement drug. Compared with the prior art, pachymaran is prepared into the pachymaran polylactic acid nanoemulsion through the multiple emulsion method, obtained nanoemulsion suspension is high in encapsulation efficiency, the drug is convenient to absorb and is slowly released, the pharmacological effect is effectively prolonged, and the pachymaran polylactic acid nanoemulsion has the immunological enhancement function and is of great significance in resisting pestilence. The pachymaran polylactic acid nanoemulsion has no side effects serving as novel traditional Chinese veterinary medicine.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a polylactic acid nanoemulsion of polysaccharides and its preparation method and application. Background technique [0002] Poria cocos is a fungus that can be used both for food and medicine, and its active ingredients mainly include: polysaccharides, alkaloids, saponins, terpenes, polyphenols and other substances. Fungal polysaccharides are metabolites isolated from fungal fruiting bodies, mycelia and their fermentation broths. They are natural polymers composed of more than 10 monosaccharides linked by glycosidic bonds. They have specific biological properties. active. Poria cocos polysaccharide is mainly composed of β-(1→3)-D-glucan, which is mainly distributed in the fruiting body, hyphae and fermentation broth of Poria cocos. Modern pharmacological studies have shown that Poria cocos polysaccharide has the effect of enhancing immune function, and can be used as an imm...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K31/715A61K47/34A61P37/04
CPCA61K9/1075A61K31/715A61K47/34
Inventor 王德云郑思思伯若楠罗莉胡元亮刘家国
Owner NANJING AGRICULTURAL UNIVERSITY
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