Chinese herbal medicine compound preparation for preventing and treating chthyophthirius multifilis for fish as well as preparation and application of Chinese herbal medicine compound preparation

A technology of small melon worm disease and compound preparations, which is applied in the field of fish parasite killing drugs, can solve the problems of high cost, high toxicity, and low activity of killing small melon worms, so as to reduce toxicity, improve drug efficacy, and facilitate industrialization. The effect of chemical production and promotion

Inactive Publication Date: 2016-10-26
JINAN UNIVERSITY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some plant species resistant to melon worms have been found. Among them, the active compound Erectin C with the best killing effect on melon worms and high safety has been isolated and identified from Baiwei. However, this active compound is in the The content in Bai Wei is only 0.0031%, the cost is too high, it is difficult to commercialize, and the alcohol crude extract of Bai Wei can greatly reduce the cost, and also has good activity of killing small melon insects, but the to...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: The killing effect of Sophora flavescens and Baiwei on the larvae of the multi-seed melon worm

[0030] (1) Preparation of ethanol extract of Chinese herbal medicine: put Sophora flavescens and Baiwei in an oven at 55°C, dry them, grind them, and pass through a 50-mesh sieve to obtain dry powders; put 50g of Sophora flavescens and Baiwei into Chinese herbal medicine extraction tanks Add 500mL of 95% ethanol, soak and extract at room temperature for 24h, repeat twice to obtain the total extract; further concentrate the total extract in a rotary evaporator, and dry the obtained concentrate in an oven at 55°C until constant Heavy.

[0031] (2) Preparation of Chinese herbal medicinal liquid: Weigh 3.84 mg of ethanol extract and place them in 1.5 mL EP tubes, dissolve them in distilled water containing 0.1% DMSO, and prepare a 2560 mg / L drug stock solution, store in a refrigerator at 4°C spare.

[0032] (3) Detection of larval activity: Add 100 μL of aqueous sol...

Embodiment 2

[0034] Example 2: Sensitivity test of Kushen Baiwei combination

[0035] (1) The preparation of Chinese herbal medicine ethanol extract is the same as in Example 1.

[0036] (2) The preparation of Chinese herbal medicinal liquid is the same as in Example 1.

[0037] (3) According to the results of the experiment on the activity of Sophora flavescens and Baiwei ethanol extract on the infective larvae of the multi-spun melon worm, two-fold concentration gradient dilutions were carried out starting from their MICs, and each Chinese herbal medicine was diluted to 4 concentration gradients. Using the checkerboard crossover method, the final detection concentration of each compound was 16 concentration gradients. At the same time, 2MIC, MIC, 1 / 2MIC and 0.1% DMSO distilled water without adding medicine were used as control groups to carry out the experiment of killing larvae in vitro. In the combination experiment of Chinese herbal medicine, the FIC index was used as the judgment b...

Embodiment 3

[0039] Embodiment 3: The killing action of Chinese herbal medicine compound to the larvae of the multi-seed melon worm

[0040] (1) Preparation of Chinese herbal medicine compound ethanol extract: Sophora flavescens 120g, Baiwei 280g, total weight 400g, placed in the extraction tank of Chinese herbal medicines, then add 4L of 95% ethanol, soak and extract at room temperature for 24h, repeat twice, to obtain the total Extract solution: the total extract solution is further concentrated under reduced pressure in a rotary evaporator, and the obtained concentrated solution is placed in an oven at 55° C. and dried to constant weight to obtain a Chinese herbal medicine compound preparation for preventing and treating multi-seed melon worm disease.

[0041] (2) Preparation of Chinese herbal medicine compound liquid: Weigh 3.84 mg of ethanol extract and put it in a 1.5 mL EP tube, dissolve it with 20 μL of absolute ethanol, then make a 2560 mg / L drug stock solution with distilled water...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lc50aaaaaaaaaa
Login to view more

Abstract

The invention discloses a Chinese herbal medicine compound preparation for preventing and treating chthyophthirius multifilis for fish as well as preparation and application of the Chinese herbal medicine compound preparation, and belongs to the technical field of fish parasite killing medicines. The compound preparation is prepared from the following raw material medicines by weight percentage: 20-40% of radix sophorae flavescentis and 60-80% of radix cynanchi atrati, wherein a preparation method of the compound preparation comprises the following steps: respectively drying and crushing the raw material medicines; screening to obtain dry powder of each raw material medicine; mixing the obtained dry powder of the raw material medicines by weight percentage; adding 95% of ethanol; soaking and extracting for twice to obtain a total extract; decompressing and concentrating the obtained total extract; and drying the obtained extract until the weight is constant, to obtain the Chinese herbal medicine compound preparation for preventing and treating chthyophthirius multifilis for fish. The Chinese herbal medicine compound preparation provided by the invention is easy to decompose, free of residues and safe to environment and people, reduces toxicity of a coarse extract of radix cynanchi atrati, improves efficacy of radix sophorae flavescentis to resist ichthyophthiriasis, greatly reduces cost, is easy to industrial production and promotion, and has broad application prospects in aquatic product.

Description

technical field [0001] The invention belongs to the technical field of drugs for killing fish parasites, and in particular relates to a compound Chinese herbal medicine preparation for preventing and treating the disease of many-child melon worms, a preparation method and an application thereof. Background technique [0002] Ichthyophthirius multifiliis is a globally distributed parasitic ciliate, which mainly parasitizes the skin, gills and fins of freshwater fish, causing Ichthyophthirius multifiliis, also known as "white spot disease", causing serious damage to the aquaculture industry. Huge economic loss. At present, chemical drugs are mainly used to prevent and control melon worm disease, but chemical drugs have the risk of polluting the environment and endangering human health, especially after malachite green is banned, there is a lack of effective drugs for the prevention and treatment of small melon worm disease on the market. Chinese herbal medicine plants have th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/489A61P33/10A23K50/80A23K10/30
CPCY02A40/818A61K36/489A61K36/27A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2300/00
Inventor 张其中林德杰
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap