Brown alga polyphenol composition with alga inhibitory activity, and preparation method and application thereof

A technology of brown algae polyphenols and compositions, which is applied in the field of brown algae polyphenols compositions and its preparation, can solve the problems that there is no in-depth discussion on the growth inhibition of microalgae, no detailed research on active substances, and the extraction method needs to be optimized. Good extraction efficiency, reduced energy consumption, and improved output value

Active Publication Date: 2016-11-09
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although it has been reported that brown algae extracts have been used in the treatment of eutrophic water bodies, their main active substances have not been studied i

Method used

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  • Brown alga polyphenol composition with alga inhibitory activity, and preparation method and application thereof
  • Brown alga polyphenol composition with alga inhibitory activity, and preparation method and application thereof
  • Brown alga polyphenol composition with alga inhibitory activity, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Preparation of Ascophyllum nodosum extracting brown algae polyphenol composition

[0038] Ascophyllum nodosum dry powder (100 mesh) was purchased from Stawest Botanicals, Canada.

[0039] The algae powder was extracted ultrasonically with 100mL of solvent, using an ultrasonic cell pulverizer (Ningbo Xinzhi), while assisting magnetic stirring, with a rotation speed of about 1,000rpm, a temperature controlled below 30°C, and 70% acetone as a solvent for extraction. After extraction, centrifuge at 10,000 rpm and 4°C for 10 minutes, take the supernatant and distill it under reduced pressure, freeze-dry it in a lyophilizer, and finally resuspend the extract in 20 mL of deionized water for use.

[0040] Table 1 Experimental results of Ascophyllum nodosum extraction conditions

[0041]

[0042] *The power is the percentage of the total power (950w) of the cell grinder

[0043] In addition, 50% and 75% acetone aqueous solution was also used as the extraction sol...

Embodiment 2

[0044] Example 2. Inhibition of the growth of "bloom" microalgae by the composition of brown algae polyphenols extracted from Ascophyllum nodosum

[0045] 2.1 Effect of Ascophyllum nodosum extract on the growth of microalgae

[0046] Two kinds of microalgae, Chlorella vulgaris and Scenedesmus sp., were respectively cultured in Erlenmeyer flasks filled with 30 mL of sterile culture solution. Except for the control, 1 mL of the Ascophyllum nodosum extract from Experiment d of Example 1 was added to each. After being cultured in a light incubator for 7 days, samples were taken to measure the cell density with a Countstar automatic cell counter (Inno-Alliance Biotech, Inc., USA) for calculating the inhibition rate. Calculated as follows:

[0047]

[0048] The two kinds of microalgae were respectively cultured in 100 mL of culture solution, and the amount of the extract added was such that its concentration in the culture solution was 3% (volume ratio). The culture conditions ...

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Abstract

The invention provides a brown alga polyphenol composition with alga inhibitory activity, and a preparation method and application thereof. According to the invention, Ascophyllum nodosum is extracted by using an organic solvent-aided low-temperature ultrasonic method; the obtained brown alga polyphenol composition exerts substantial inhibitory effect on the growth of bloom microalgae, specifically inhibitory effect on cell division and synthesis of chlorophyll a; and inhibition of the growth of microalgae is overall effect of inhibition action in a plurality of aspects. The invention also provides a method for comprehensive utilization of Ascophyllum nodosum. The method can greatly improve the output value of Ascophyllum nodosum and reduce energy consumption.

Description

technical field [0001] The invention relates to the field of environmental engineering, in particular to a brown algae polyphenol composition and its preparation method and application. Background technique [0002] Brown algae are higher multicellular algae and belong to eukaryotic cell organisms. There are about 1,500 to 2,000 species of brown algae in the world, mainly distributed in the waters near the mainland, and freshwater species are rare. The appearance of brown algae can vary from dark brown to olive green, depending on the ratio of fucoxanthin to chlorophyll. Common brown algae include Ascophyllum, Sargassum, and Laminaria. In recent years, it has been confirmed that some components in brown algae have antibacterial, antifungal, antiviral, antioxidative, anti-inflammatory, antitumor and growth-promoting activities, so according to these biological characteristics, brown algae are gradually developed as fertilizers, feed, Raw materials for pharmaceuticals, cosm...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P13/00C08B37/00
CPCA01N65/03C08B37/00
Inventor 吴华南石萍耿旭
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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