Paris forrestii (Takht.) H. Li monomer saponin combination, pharmaceutical composition thereof and application thereof in pharmaceutical industry

A technology of long-column C. pauli and C. saponin, which is applied in the field of medicine, can solve the problems such as the prevention and treatment of tumors that are resistant to total saponins of C. pachyrhizi.

Inactive Publication Date: 2016-11-09
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the prevention and treatment of drug-resist

Method used

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  • Paris forrestii (Takht.) H. Li monomer saponin combination, pharmaceutical composition thereof and application thereof in pharmaceutical industry
  • Paris forrestii (Takht.) H. Li monomer saponin combination, pharmaceutical composition thereof and application thereof in pharmaceutical industry
  • Paris forrestii (Takht.) H. Li monomer saponin combination, pharmaceutical composition thereof and application thereof in pharmaceutical industry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. The preparation of the active part PFE-PT3 of the present invention and the separation of its chemical components:

[0030] Preparation of PFE-PT3: dried rhizomes (4.4kg) of Longzhuzhonglou [Paris forrestii(Takht.)H.Li], crushed and soaked in 95% ethanol (13L) for 7 days, filtered, and the filter residue continued extraction. A total of 4 extractions were performed, the filtrate was combined, and the solvent was recovered to obtain 550 g of extract (number PFE-PT1). The PFE-PT1 was made into a suspension with water (2L), extracted with ethyl acetate (2L×3), and the ethyl acetate was recovered to obtain 19 g of ethyl acetate extraction site (number PFE-PT2). The mother liquor after ethyl acetate extraction is continued to be extracted with n-butanol (2L×3) to recover the n-butanol to obtain 290 g of the n-butanol extraction part (number PFE-PT3).

[0031] Separation of chemical components contained in PFE-PT3:

[0032] PFE-PT3 weighing 180g was eluted and segmented with a ...

Embodiment 2

[0041] The cytotoxic activity was determined by modified MTT method. The cells in the logarithmic growth phase were seeded in a 96-well culture plate. 5% CO 2 After culturing in the incubator for 24 hours, add the test sample. An equal volume of saline was added to the negative control group, and doxorubicin or paclitaxel was added to the positive control group. After culturing for 48h, add MTT, 10μL / well. After continuing to incubate for 4 hours, add triple solution [10% SDS-5% isobutanol-0.012mol / l HCL(W / V / W)], 100μL / well. After standing overnight, use a microplate reader at 570nm, 630nm double Measure the OD value of each well at the wavelength. Actual OD value = experimental well OD value-blank control well OD average value; cell proliferation inhibition rate (%) = 100%-(actual OD value of sample hole / actual OD value of negative control well) × 100%; use GWBASIC software Calculate the IC of the sample 50 value. Resistance Index (Resistance Index, RI) = IC of resista...

Embodiment 3

[0049] Preparation of medicines for cancer treatment: Longzhuzhonglou total saponins PFE-PT3, add sodium carboxymethyl starch, appropriate amount of starch, starch paste granulation, dry, whole, add appropriate amount of talcum powder, mix well, press tablet (each 0.5 g, equivalent to 3g of Longzhuzhonglou rhizome), 2 tablets each time, 3 times a day.

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Abstract

The invention provides Paris forrestii (Takht.) H. Li total saponin PFE-PT3 and monomer saponin combination PFE-PT65, and provides a pharmaceutical composition using the combination as an active ingredient, and application in the preparation of a drug for preventing or treating drug-resistant breast cancer and drug-resistant nasopharyngeal carcinoma. The Paris forrestii (Takht.) H. Li total saponin PFE-PT3 and monomer saponin combination PFE-PT65 has an obvious inhibiting effect on adriamycin-resistant human breast cancer MCF-7 / ADM cells, with a resistance index of less than 2, and has an obvious inhibiting effect on paclitaxel-resistant human nasopharyngeal carcinoma KBvin cell growth, with a resistance index of less than 2.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and specifically relates to the total saponins PFE-PT3 and its monomer saponin combination PFE-PT65, its preparation method and its application in the preparation of drugs for preventing or treating drug-resistant tumors. Background technique: [0002] Paris forrestii (Takht.) H.Li is a plant of the genus Trillium, whose rhizome is used as medicine. It has the effects of clearing heat and detoxification, reducing swelling and pain, cooling the liver and relieving convulsions, and is used to treat carbuncle sores. , Unnamed swollen poison, snake bite, sore throat, mumps, tonsillitis, and macrocephaly (Zhu Zhaoyun. Yunnan Natural Medicines Illustrated Book. Volume 6. Kunming: Yunnan Science and Technology Press, 2010: 127). The patent application "A plant medicine for the treatment of cancer and its preparation method and application" (publication number: CN104706896A) discloses that the PFE-PT3 part ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048A61K36/896
Inventor 王跃虎周轶平梅任强陈英杰杨珺施敏罗吉凤杨永平李恒
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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