Articular cavity extracellular matrix polysaccharide nanometer injection and preparation method thereof

A technology for injection and joint cavity, applied in the field of extracellular matrix-like polysaccharide nano-injection for joint cavity and its preparation, can solve the problems of failure to provide and difficult to effectively promote cell adhesion, proliferation and differentiation, and achieve High safety, good stability, strong osteoinductive effect

Inactive Publication Date: 2016-11-09
上海建华精细生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of agarose hydrogel simulates the water environment of the natural extracellular matrix, it fails to provide the functional, structural proteins and nutrients contained in the extracellular matrix, so it is difficult to effectively promote cell adhesion, proliferation and differentiation

Method used

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  • Articular cavity extracellular matrix polysaccharide nanometer injection and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Injection formulation

[0025] A kind of extracellular matrix-like polysaccharide nano-injection for joint cavity, which comprises the following components: 3.5wt% sodium hyaluronate, 3wt% collagen, 1wt% human bone morphogenic protein-7, 1wt% sulfuric acid Chondroitin, 0.5wt% heparan sulfate, 2wt% glucosamine sulfate, 0.5wt% chitosan, 0.5wt% isotonic regulator, pH regulator and deionized water, the addition of pH regulator is The pH of the injection is 6.0.

[0026] Wherein, the pH of the injection is adjusted by mixing phosphate buffer and borate buffer in any proportion.

[0027] The particle diameter of the nano microspheres in the injection solution is 1000nm.

[0028] 2. Preparation method of injection

[0029] (1) Dissolve sodium hyaluronate in deionized water to make sodium hyaluronate aqueous solution, dissolve collagen in deionized water to make collagen aqueous solution, and dissolve chitosan in deionized water to make a few Tetraose aqueous solution; ...

Embodiment 2

[0035] 1. Injection formulation

[0036] An extracellular matrix-like polysaccharide nano-injection for joint cavity, which comprises the following components: 1wt% sodium hyaluronate, 2wt% type II collagen, 0.5wt% human bone morphogenic protein-7, 0.2wt% % of chondroitin sulfate, 0.1wt% heparan sulfate, 1wt% glucosamine sulfate, 5wt% chitosan, 0.8wt% sodium chloride, pH regulator and deionized water, the addition of pH regulator is Make the pH of the injection solution 7.0.

[0037] Wherein, the pH of the injection is adjusted with a phosphate buffer.

[0038] The particle size of the nano microspheres in the injection is 500nm.

[0039] 2. Preparation method of injection

[0040] (1) Dissolve sodium hyaluronate in deionized water to make sodium hyaluronate aqueous solution, dissolve collagen in deionized water to make collagen aqueous solution, and dissolve chitosan in deionized water to make a few Tetraose aqueous solution;

[0041] (2) Mix sodium hyaluronate aqueous s...

Embodiment 3

[0046] 1. Injection formulation

[0047] An extracellular matrix-like polysaccharide nano-injection for joint cavity, which comprises the following components: 4wt% sodium hyaluronate, 5wt% type II collagen, 1.5wt% human bone morphogenic protein-7, 2wt% Chondroitin sulfate, 0.5wt% heparan sulfate, 4wt% glucosamine sulfate, 3wt% chitosan, 0.2wt% sodium chloride, pH regulator and deionized water, the addition of pH regulator is to make The pH of the injection is 6.5.

[0048] Wherein, the pH of the injection is adjusted with a boric acid buffer.

[0049] The particle diameter of the nano microspheres in the injection solution is 10nm.

[0050] 2. Preparation method of injection

[0051] (1) Dissolve sodium hyaluronate in deionized water to make sodium hyaluronate aqueous solution, dissolve collagen in deionized water to make collagen aqueous solution, and dissolve chitosan in deionized water to make a few Tetraose aqueous solution;

[0052] (2) Mix sodium hyaluronate aqueou...

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Abstract

The invention belongs to the field of biological materials, and particularly relates to an articular cavity extracellular matrix polysaccharide nanometer injection and a preparation method thereof. The injection is prepared from, by weight, 1-4% of sodium hyaluronate, 2-5% of collagen, 0.5-1.5% of human bone morphogenetic proteins, 0.2-3% of chondroitin sulfate, 0.1-1% of heparin sulfate, 1-4% of glucosamine sulfate, 0.1-5% of chitosan, 0.2-0.8% of isoosmotic adjusting agent, a pH regulator and deionized water. Due to the addition amount of the pH regulator, the pH of the injection is 6.0-7.0. The prepared injection can effectively protect cartilago articularis and has a slow-release anti-inflammatory effect.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an extracellular matrix-like polysaccharide nano-injection for joint cavity and a preparation method thereof. Background technique [0002] Articular cartilage belongs to hyaline cartilage, without innervation and blood vessel distribution. The damage caused by articular cartilage has limited self-repair ability. After the articular cartilage is damaged, the absorption and cushioning effect of the stress is reduced, and the damage will be progressively aggravated. At the same time, the damaged articular cartilage has the risk of developing severe osteoarthritis. At present, the repair methods of articular cartilage defects mainly include: joint washing and debridement, microfracture bone marrow stimulation, osteochondral transplantation and autologous chondrocyte transplantation. The above technologies have their own advantages and disadvantages in clinical applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61K9/10A61P19/02A61P29/00A61K38/18A61K31/737A61K31/728A61K31/727A61K31/722A61K31/7008A61K9/19
CPCA61K31/728A61K9/0019A61K9/10A61K9/19A61K31/7008A61K31/722A61K31/727A61K31/737A61K38/1875A61K38/39A61K47/02A61K2300/00
Inventor 张军东陈晚华吴剑英常臻
Owner 上海建华精细生物制品有限公司
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