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Skin tissue engineering double-layer film material and preparation method thereof

A technology of skin tissue engineering and double-layer membrane, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of unsatisfactory performance and high cost of scaffold materials, achieve good biocompatibility and biological activity, and require low equipment , the effect of simple method

Inactive Publication Date: 2016-11-09
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the performance of the existing skin tissue engineering scaffold material is not ideal, and the cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Mix glycerin, glacial acetic acid, and distilled water at a volume ratio of 0.5:1:25 to obtain mixed solution A, and add chitosan to mixed solution A at a ratio of 12g / L and stir Make it fully dissolved to obtain mixed solution B;

[0024] (2) Filter the mixed solution B prepared in step (1), degas the filtrate for 35 hours under a negative pressure of 400 mmHg, and then make a film by casting method, and the film has a concentration of 2.5% by mass Cross-linking in the cross-linking solution of NaOH solution for 2 hours, then rinsing with distilled water for 6 times, freeze-drying to obtain chitosan film;

[0025] (3) Mix glycerin, lactic acid, and distilled water at a volume ratio of 0.5:1.6:50 to obtain mixed solution C, and add konjac glucomannan to mixed solution C at a ratio of 10g / L Stir to make it fully dissolve to obtain mixed solution D;

[0026] (4) Filter the mixed solution D prepared in step (3), degas the filtrate for 35 hours under a negative pressu...

Embodiment 2

[0030] (1) Mix glycerin, glacial acetic acid, and distilled water at a volume ratio of 0.7:3:100 to obtain mixed solution A, and add chitosan to mixed solution A at a ratio of 23g / L and stir Make it fully dissolved to obtain mixed solution B;

[0031] (2) Filter the mixed solution B prepared in step (1), degas the filtrate for 50 hours under a negative pressure of 500 mmHg, and then make a film by casting method, and the film has a mass percentage concentration of 5% Cross-linking in the cross-linking solution of NaOH solution for 5 hours, then rinsing 7 times with distilled water, freeze-drying to obtain chitosan film;

[0032] (3) Mix glycerin, lactic acid, and distilled water at a volume ratio of 1.0:1:100 to obtain mixed solution C, and add konjac glucomannan to mixed solution C at a ratio of 15g / L Stir to make it fully dissolve to obtain mixed solution D;

[0033] (4) Filter the mixed solution D prepared in step (3), degas the filtrate for 45 hours under a negative pres...

Embodiment 3

[0037] (1) Mix glycerin, glacial acetic acid, and distilled water at a volume ratio of 1:5:200 to obtain mixed solution A, and add chitosan to mixed solution A at a ratio of 30g / L and stir Make it fully dissolved to obtain mixed solution B;

[0038] (2) Filter the mixed solution B prepared in step (1), degas the filtrate for 30 hours under a negative pressure of 760 mmHg, and then make a membrane by casting method, and the concentration of the membrane in mass percentage is 8%. Cross-linking in the cross-linking solution of NaOH solution for 3 hours, then rinsing with distilled water 8 times, freeze-drying to obtain chitosan film;

[0039] (3) Mix glycerin, lactic acid, and distilled water at a volume ratio of 1.5:3:160 to obtain mixed solution C, and add konjac glucomannan to mixed solution C at a ratio of 20g / L Stir to make it fully dissolve to obtain mixed solution D;

[0040] (4) Filter the mixed solution D prepared in step (3), degas the filtrate for 50 hours under a ne...

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Abstract

The invention discloses a skin tissue engineering double-layer film material and a preparation method thereof. The preparation method comprises the steps that degradable materials including chitosan and konjac glucomannan which have the good biocompatibility are adopted as raw materials and prepared into mixed solutions separately, film forming is conducted on the mixed solutions through a tape casting method separately, two layers of films are bonded together by adopting a konjac glucomannan solution with the certain concentration as a bonding agent, and then the chitosan-konjac glucomannan double-layer film material is prepared. The material has the advantages of being simple in preparation process, low in cost, adjustable in degradation rate, good in biocompatibility and the like; early cell experiments and animal experiments show that the double-layer film material can be suitable for organisms and is a degradable multifunctional composite material capable of promoting skin repairing.

Description

technical field [0001] The invention relates to a skin tissue engineering double-layer film material and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] The skin is the largest organ of the human body and is the barrier between the human body and the external environment. It has important functions such as excretion, sensation, prevention of water evaporation, temperature regulation, and immunity. The importance of the skin to the human body is self-evident. However, in real life, skin defects are often caused by burns, trauma, diabetic chronic ulcers and other reasons. Any full-thickness skin defect larger than 4cm in diameter, the human body will not be able to fully heal itself. In these cases, there is a need for a suitable wound repair material to promote wound healing. Tissue-engineered skin has been used clinically for 25 years, and it has brought many benefits to the rehabilitation of patients with large-a...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/58A61L27/60
CPCA61L27/20A61L27/58A61L27/60A61L2430/34C08L5/08C08L5/02
Inventor 陈庆华雷云冷崇燕汪虹黄洁
Owner KUNMING UNIV OF SCI & TECH
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