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A kind of compound phosphorus dissolving fungus and its application in agricultural production

A technology of phosphate inoculant and phosphate-solubilizing bacteria, applied in the field of biological fertilizer, can solve the problems of non-renewable phosphate rock resources, soil compaction and degradation, low planting efficiency, etc. Effect

Active Publication Date: 2019-03-26
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the amount of phosphorus fertilizer application increases year by year, the phenomenon of phosphorus accumulation in farmland soil is becoming more and more serious, which in turn leads to soil compaction degradation and low planting efficiency. At the same time, phosphorus runoff and leaching have become the main pollution sources of water eutrophication
Moreover, phosphate rock resources are non-renewable. Therefore, how to increase the content of available phosphorus in the soil and make full and effective use of phosphate fertilizers has become a common concern in the agricultural field at home and abroad.

Method used

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  • A kind of compound phosphorus dissolving fungus and its application in agricultural production
  • A kind of compound phosphorus dissolving fungus and its application in agricultural production
  • A kind of compound phosphorus dissolving fungus and its application in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mensuration of embodiment 1 composite phosphate solubilizing bacteria agent to soil phosphorus decomposing effect

[0021] 1. Preparation of Aspergillus niger FMJ1 bacterial powder:

[0022] Add 1% of Aspergillus niger FMJ1 bran koji seeds into sterilized solid fermentation medium, culture at 30°C for 72h, dry in an oven at 40°C, and pulverize. The number of effective viable bacteria of Aspergillus niger in the obtained bacteria powder was 3×10 9 CFU / g.

[0023] 2. Phytase:

[0024] The pH range is 2-8, the optimum pH is 5.5, and the enzyme activity is 20000U / g.

[0025] 3. Preparation of sterile soil:

[0026] Pass the soil with a pH value of 8.2 through a mesh sieve with a pore size of 8 mm, put it in a white cotton bag, spread it into a thin layer of about 3-4 cm, and sterilize at 121 ° C for 2 hours.

[0027] 4. Weigh 4 parts of the sterilized soil, each 100g, put them into the Erlenmeyer flask, and do the following:

[0028] (1) Treatment group 1: insert ...

Embodiment 2

[0038] Phosphorus-dissolving effect of embodiment 2 compound phosphate-dissolving bacteria agent in field cultivation experiment of rapeseed

[0039] 1. Experimental location: rape planting greenhouse in Xifu Town, Chengyang District, Qingdao City, the overall soil condition is relatively uniform.

[0040] 2. Composite phosphate solubilizer sample: the number of spores of Aspergillus niger is 10 9 CFU / g, phytase activity is 7000U / g. .

[0041] 3. Experimental process:

[0042] A square area of ​​2m × 2m was selected as an experimental area, and a total of 40 experimental areas were set up, and a distance of 1 meter was maintained between each experimental area.

[0043] There are 4 groups in the experiment:

[0044] (1) Blank control group: without adding any substance;

[0045] (2) Treatment group 1: 10 g / m in each experimental plot 2 The proportion of uniform application of Aspergillus niger FMJ1 powder (spore number 10 9 CFU / g), then effectively mix the 5-10cm thick ...

Embodiment 3

[0053] Application of embodiment 3 compound phosphate solubilizing bacteria agent in inorganic fertilizer

[0054] 1. Experimental location: Pingdu South Village, Qingdao City, the overall soil condition is relatively uniform.

[0055] 2. Experimental samples:

[0056] 1) Composite phosphate solubilizer sample: the number of spores of Aspergillus niger is 3×10 9 CFU / g, phytase activity is 10000U / g.

[0057] 2) Traditional compound fertilizer sample: Stanley compound fertilizer, where N-P 2 o 5 -K 2 O, 15-15-15, total nutrients ≥ 45%.

[0058] 3. Experimental process:

[0059] 1) The soil to be tested is brown soil, the pH value is 7.0, and the soil available phosphorus content is 15.7mg / kg.

[0060] 2) Corn variety: Yongyu 995.

[0061] 3) The area of ​​the experimental plot is 40m 2 , a total of 5 treatments, each treatment set up 3 parallels, and each experimental plot was randomly arranged.

[0062] Control group: only traditional compound fertilizer was applied, ...

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Abstract

The invention relates to the field of biological fertilizers, in particular to a composite phosphate-dissolving bacterium agent capable of increasing the content of available phosphate in soil, and an application of the composite phosphate-dissolving bacterium agent in agricultural production. The composite phosphate-dissolving bacterium agent is prepared through compounding microorganisms with a phosphate dissolving function and an enzymic preparation. Inorganic phosphate fixed in the soil can be dissociated, and organic phosphate which is not easy to naturally absorb by plants can be efficiently transformed, so that the usage quantity of chemical phosphate fertilizers can be effectively reduced, even the usage of the chemical fertilizers can be completely replaced, increase of planting benefits is facilitated, and environmental pollution is reduced.

Description

technical field [0001] The invention relates to the field of biological fertilizers, in particular to a compound phosphate-dissolving bacteria agent for increasing soil available phosphorus content and its application in agricultural production. Background technique [0002] Phosphorus is an important component of the plant nucleus, and plays an important role in cell division and the differentiation and development of various plant organs and tissues, especially flowering and fruiting. It is an indispensable element for physiological metabolic activities in plants. Phosphorus also has a good effect on improving the disease resistance, cold resistance and drought resistance of plants. In leguminous crops, phosphorus can promote the development of root nodules, improve the nitrogen fixation capacity of root nodules, and indirectly improve the nutritional status of plants. Phosphorus also has the effect of promoting root development, especially the growth of lateral roots and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/14C05G3/04C05F11/08C09K101/00C05G3/80
CPCC05F11/08C05G3/80C09K17/14C09K2101/00
Inventor 刘鲁民宗睿刘金
Owner QINGDAO VLAND BIOTECH GRP