A method for simultaneously extracting and measuring tobacco cembratrienediol, vitamin E, and phytosterols
A phytosterol and vitamin technology, applied in the field of phytosterol, vitamin E, simultaneous extraction and determination of tobacco cerebrotriene glycol, can solve the problems of high consumption of organic solvents, complicated processing methods, etc., and achieves less solvent consumption and more peaks. , the effect of high efficiency
Inactive Publication Date: 2019-04-16
TOBACCO RES INST CHIN AGRI SCI ACAD
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Problems solved by technology
[0005] Aiming at the technical problems that the determination of the beneficial active components of tobacco in the prior art is carried out independently and the pretreatment method is cumbersome, and the consumption of organic solvents is large, the purpose of the present invention is to provide a method for simultaneously extracting and measuring tobacco CBT-diol, vitamin E, the method of phytosterol
Method used
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Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0048] Weigh 0.1g of the prepared flue-cured tobacco powder sample, put it in a centrifuge glass tube with a stopper, add 0.2g of vitamin C, mix well, add 2mL of 95% ethanol, put it into a constant temperature ultrasonic extractor, and extract 10mim of vitamin C by ultrasonic extraction at 60°C. C dissolves, take out the centrifuge tube, add 2mL of 2M potassium hydroxide-95% ethanol solution, continue to put it into a constant temperature ultrasonic extractor, extract 40mM by ultrasonic at 60°C, cool, add 5mL of n-hexane, 16mL of deionized water, shake, Centrifuge; take the n-hexane layer and add 0.1g of anhydrous sodium sulfate, pass through a 0.22um organic filter membrane, inject it into a gas-phase sampling bottle, and wait for the test. like figure 1 , is the liquid chromatogram of the sample measuring solution.
Embodiment 2
[0050] Weigh and weigh 0.1 g of the modulated oriental tobacco leaf powder sample, and place it in a centrifuge glass tube with a stopper. The following steps are as in Example 1.
Embodiment 3
[0052] Weigh and weigh 0.1 g of the dried oriental tobacco fireworks sample and place it in a centrifuge glass tube with a stopper. The following steps are as in Example 1.
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The invention discloses a method for extracting and measuring cembratriene diol (CBT-diol), vitamin E and phytosterol in tobacco at the same time. The method concretely comprises the following steps: weighing a tobacco powder sample and putting the sample in a centrifugal glass tube provided with a plug; adding vitamin C as an antioxidant; adding an ethanol solution of potassium hydroxide as an extracting agent and a saponifier; extracting and saponifying a target compound under a constant-temperature ultrasonic condition; adding n-hexane as an extracting agent; performing vibration and uniform mixing; adding deionized water; performing vibration and centrifugalization; allowing the obtained solution to be layered; adding anhydrous sodium sulfate in a n-hexane layer; performing filtration through an organic filter membrane; and injecting the obtained product in a gas sampler vial for measurement. According to the method, CBT-diol, vitamin E, campesterol, stigmasterol and sitosterol can be extracted and measured at the same time. The method is simple in steps, less in solvent consumption, and is suitable for batch measurement of active ingredients in a tobacco sample. The method is eco-friendly and safe and is high in efficiency. The detection efficiency of target objects is improved, and the high sensitivity and low detection limit can be obtained. The method is good in repeatability and stability.
Description
technical field [0001] The invention relates to the technical field of extracting and measuring beneficial active components of tobacco, in particular to a method for simultaneously extracting and measuring tobacco cembratrienediol, vitamin E and phytosterol. Background technique [0002] Cembratriene diol (CBT-diol) is the main chemical component of tobacco glandular hair secretion, and also an important precursor substance that affects the aroma quality of cigarettes. Studies at home and abroad have shown that CBT-diol also has various biological activities such as anti-tumor, neuroprotective agent, and insecticide. Both vitamin E and sterols are plant natural active ingredients widely used in the fields of medicine, health care products, and food, and are also important chemical ingredients that affect the quality and safety of tobacco. For traditional cigarettes, only a small part of vitamin E and sterols are directly transferred to the smoke during the smoking process ...
Claims
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/025G01N2030/062
Inventor 张怀宝杜咏梅刘翠翠刘新民付秋娟王爱华谭家能张鹏
Owner TOBACCO RES INST CHIN AGRI SCI ACAD