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Compound biological feed additive and preparation method thereof

A biological feed and additive technology, applied to animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of difficult control of reaction conditions, low conversion efficiency, high cost, etc., to reduce the use of antibiotics and high conversion rate , anti-osteoporosis effect

Inactive Publication Date: 2016-11-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have shortcomings such as low conversion efficiency, high cost, and difficult control of reaction conditions, and it is difficult to solve the problem of large-scale promotion and application of soybean isoflavone feed additives in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Select soybean meal, corn and bran to grind through 60 meshes, and mix them according to the weight ratio of soybean meal, corn and bran at 60%, 35% and 5% to obtain the main fermentation material;

[0035] 2. Prepare probiotic starter: select Aspergillus niger, Lactobacillus plantarum, Bacillus licheniformis, Saccharomyces boulardii and Trichoderma, and mix according to the following bacteria ratio: Aspergillus niger 35%; Lactobacillus plantarum 45%, Bacillus licheniformis 10%, Saccharomyces boulardii 6%, Trichoderma 4%, the total live bacteria content is 10.2 billion / gram.

[0036] 3. Preparation of culture medium: according to the following mass percentage ratio, molasses 2%, diammonium citrate 0.2%, K 2 HPO 4 0.15%, KH 2 PO 4 0.2%, MgSO 4 ·7H 2 O 0.04%, MnSO 4 ·H 2 O 0.025%, ferrous sulfate 0.04%, zinc sulfate 0.05%, sodium selenite 0.0006%, lipoic acid 0.02%, sodium acetate 0.1%, VB 1 0.05%, the rest is sterilized water, dissolve and mix well.

[0037...

Embodiment 2

[0041] Select soybean meal, corn and bran to grind through 60 meshes, and mix them according to the weight ratio of soybean meal, corn and bran at 55%, 30% and 15% to obtain the fermentation main ingredient;

[0042] Preparation of probiotic starter: select Aspergillus niger, Lactobacillus plantarum, Bacillus licheniformis, Saccharomyces boulardii and Trichoderma, and mix according to the following bacteria ratio: Aspergillus niger 15%; Lactobacillus plantarum 65%, Bacillus licheniformis 7% , Saccharomyces boulardii 10%, Trichoderma 3%, the total content of live bacteria is 9.8 billion / g.

[0043] Preparation of culture medium: according to the following mass percentage ratio, molasses 4%, diammonium citrate 0.1%, K 2 HPO 4 0.2%, KH 2 PO 4 0.1%, MgSO 4 ·7H 2 O 0.04%, MnSO 4 ·H 2 O 0.03%, ferrous sulfate 0.025%, zinc sulfate 0.02%, sodium selenite 0.0005%, lipoic acid 0.05%, sodium acetate 0.2%, VB 1 0.06%, the rest is sterilized water, dissolve and mix well.

[004...

Embodiment 3

[0048] Select soybean meal, corn and bran to grind through 60 meshes, and mix them according to the weight ratio of soybean meal, corn and bran at 75%, 15% and 10% to obtain the fermentation main ingredient;

[0049] Preparation of probiotic starter: select Aspergillus niger, Lactobacillus plantarum, Bacillus licheniformis, Saccharomyces boulardii and Trichoderma, and mix according to the following bacteria ratio: Aspergillus niger 32%; Lactobacillus plantarum 35%, Bacillus licheniformis 20% , Saccharomyces boulardii 5%, Trichoderma 8%, the total content of live bacteria is 10.1 billion / gram.

[0050] Preparation of culture medium: according to the following mass percentage ratio, molasses 1%, diammonium citrate 0.2%, K 2 HPO 4 0.1%, KH 2 PO 4 0.4%, MgSO 4 ·7H 2 O 0.02%, MnSO 4 ·H 2 O 0.04%, ferrous sulfate 0.03%, zinc sulfate 0.03%, sodium selenite 0.0008%, lipoic acid 0.06%, sodium acetate 0.4%, VB 1 0.07%, the balance is sterilized water, dissolve and mix, and ad...

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Abstract

The invention discloses a compound biological feed additive and a preparation method thereof. The preparation method comprises a step (1) of selecting aspergillus niger, lactobacillus plantarum, bacillus licheniformis, saccharomyces boulardii and trichoderma to prepare a compound probiotics leavening agent; a step (2) of making molasses, ammonium citrate dibasic, K2HPO4, KP2PO4, magnesium sulfate-water (1 / 7), manganese sulfate-water (1 / 1), ferrous sulfate, zinc sulfate, sodium selenite, lipoic acid, sodium acetate, VB1 and water into culture solution; a step (3) of mixing the leavening agent and the culture solution, and obtaining a compound probiotic fermented liquid; a step (4) of mixing and then inoculating bean pulp, corn, bran and soy isoflavone crude extract; and a step (5) of adjusting moisture and performing fermentation to obtain the compound biological feed additive. The preparation method is simple, the content of soy isoflavone aglycone and beneficial microorganisms and metabolites thereof in the obtained compound biological feed additive is high, and the compound biological feed additive is non-toxic and free of medicine resistance and side effect.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a compound biological feed additive and a preparation method thereof. Background technique [0002] The side effects of feed antibiotics have attracted increasing attention, and antibiotic-free breeding has become a hot spot. The production of prebiotics through the fermentation of specific probiotics contains a large number of beneficial microorganisms and their metabolites, such as antimicrobial peptides, digestive enzymes, organic acids, antioxidants, etc., and inhibits the growth of harmful bacteria through the biological competitive exclusion of the animal digestive tract. Growth, forming dominant flora or enhancing non-specific immune function to prevent diseases, thereby promoting animal growth and improving feed conversion rate. Prebiotic feed additive is a natural biologically active preparation with the characteristics of non-toxicity, no drug resistance, and no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/37A23K20/121A23K10/33A23K20/26A23K20/20A23K20/174A23K20/105A23K10/12
CPCA23V2400/169Y02P60/87
Inventor 徐建雄任慧鹏余思佳
Owner SHANGHAI JIAO TONG UNIV
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