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Rapid detection method for botrytis cinerea on drug resistance of multiple agents

A technology for botrytis cinerea and detection methods, which is applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of laboriousness, limited number of one-time detection, time-consuming, etc., and achieve remarkable scalability and practical value Significant, easy-to-operate effect

Inactive Publication Date: 2016-12-07
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of science and technology, the research on the drug resistance of pathogenic bacteria has become a hot issue. Modern molecular detection methods are changing with each passing day. The resistance to fungicides can be detected by molecular diagnostic technology at the nucleic acid level, but the mutation sites controlling resistance in the genome are not Definite pathogenic bacteria or units that do not have the conditions for molecular detection of drug resistance can only use traditional pathogenic bacteria resistance detection technology
[0003] Traditional pathogenic bacteria resistance detection techniques are mainly mycelia growth method and spore germination method, which require field sampling, laboratory separation and purification, and inoculation on drug-containing medium or plant tissue sprayed with drugs. This process is time-consuming, laborious, and Limited number of one-time tests

Method used

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  • Rapid detection method for botrytis cinerea on drug resistance of multiple agents
  • Rapid detection method for botrytis cinerea on drug resistance of multiple agents
  • Rapid detection method for botrytis cinerea on drug resistance of multiple agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A) Test site: Strawberry Dapeng, a farmer in Baitu Town, Jurong City, Jiangsu Province

[0021] B) Test time: April 2016

[0022] C) Purpose of the test: To detect the resistance level of 50 strains of Botrytis cinerea to carbendazim, procymidone, diethofencarb, pyraclostrobin and azoxystrobin.

Embodiment approach

[0024] (1) Botrytis cinerea detection kit is prepared: the botrytis cinerea detection kit is prepared in the laboratory of Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu: at first determine the differential measurement and medium type of the test agent (see Table 1 for details), Select a 6-well sterilized cell culture plate (see figure 1 ), the sterilized medium was prepared into a drug-containing medium, which was added to the culture plate, and a drug-containing medium was added to each well, and the PDA medium was used as a blank control, wherein azoxystrobin and pyraclostrobin were To prevent bypass oxidation, the medium contains SHAM 100mg L -1 , seal the prepared detection kit, and store it in a refrigerator at 4°C for later use.

[0025] Table 1 Distinguishing metering and culture medium types of medicaments

[0026]

[0027]

[0028] (2) Sampling and detection: take 50 above-mentioned kits, put them into a sorting box, and bring the...

Embodiment 2

[0034] A) Test location: Laofang Vineyard, Jurong City, Jiangsu Province

[0035] B) Test time: May 2016

[0036] C) Purpose of the test: To detect the effects of 50 strains of botrytis cinerea on carbendazim, procymidone, diethofencarb, prochloraz, pyraclostrobin, azoxystrobin, boscalid, fluopyram, fluorine The drug resistance level of 11 kinds of pesticides such as pyraclostrobin, pyrimethanil and fludioxonil.

[0037] D) Implementation method:

[0038] (1) Botrytis cinerea detection kit is prepared: the botrytis cinerea detection kit is prepared in the laboratory of Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu: at first determine the differentiating measurement and medium type of the test agent (see Table 3 for details), Select a 12-well sterilized cell culture plate (see figure 2 ), the sterilized medium was prepared into a drug-containing medium, which was added to the culture plate, and a drug-containing medium was added to each well, a...

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Abstract

The invention discloses a rapid detection method for botrytis cinerea on drug resistance of multiple agents. The rapid detection method includes: firstly, preparing a botrytis cinerea detection kit; secondary, performing field sample for in-situ detection; finally, performing rapid detection on drug resistance of the multiple agents by determining whether or not bacterial strains generate drug resistance according to normal growth of pathogenic bacteria. With the method, shortcomings that a traditional drug resistance detecting technology of the pathogenic bacteria needs field sample, laboratory separation and purification, time consumption and labor consumption in inoculating the pathogenic bacteria into drug-containing medium or plant tissue sprayed with the agents and one-time detection is limited in number are overcome; the rapid detection method has the advantages of being timesaving and laborsaving, convenient in operation, free of sampling, capable of detecting the agents in place simultaneously rapidly and accurately, and remarkable in practical value and generalizability.

Description

technical field [0001] The invention relates to the technical field of detection of drug resistance of pathogenic bacteria, in particular to a rapid detection method for the resistance of Botrytis cinerea to multiple agents. Background technique [0002] Plant pathogenic bacteria resistance refers to the long-term adaptability of pathogenic bacteria through heredity and variation under the pressure of selection by a single agent or similar agents. With the development of science and technology, the research on the drug resistance of pathogenic bacteria has become a hot issue. Modern molecular detection methods are changing with each passing day. The resistance to fungicides can be detected by molecular diagnostic technology at the nucleic acid level, but the mutation sites controlling resistance in the genome are not Definite pathogenic bacteria or units that do not have the conditions for molecular detection of drug resistance can only use traditional pathogenic bacteria re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 肖婷杨敬辉许媛
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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