Culture medium for isolated culture of mycoplasma dispar and preparation method thereof

Abstract

The invention discloses a culture medium for isolated culture of mycoplasma dispar. Each 1000 ml of the culture medium comprises: 8.5 g of PPLO broth, 8.5 g of brain heart infusion, 2.0 g of glucose, 500 ml of a Hank's balanced salt solution, 60 ml of a 25% fresh yeast extraction liquid, 100 ml of sterile swine serum, 100 ml of sterile horse serum, 100 mg of ampicillin, and 1 ml of 10% thallium acetate. The invention also provides a preparation method of the culture medium. The culture medium, compared with culture mediums in the prior art, reaches 10<10> CCU / ml in living bacteria titer, while the growth titers of the culture mediums in the prior art are 10<7>-10<9> CCU / ml. In subculture of the mycoplasma dispar, the culture medium requires 2-3 days for one passage, while the culture mediums in the prior art require 3-5 days usually for one passage; the culture medium is fully proved to have quick growth and high culture titer, and is suitable for the isolated culture of the mycoplasma dispar.

Description

technical field [0001] The invention relates to the technical field of veterinary biology, in particular to a culture medium for the isolation and cultivation of Mycoplasma heteroplasma and a preparation method thereof. Background technique [0002] Mycoplasma heterogenes ( Mycoplasma dispar ), formerly known as Mycoplasma heteromycetes. Gourlay RN and Leach RH first isolated the bacterium from the lung of a calf suffering from pneumonia in England in 1969 (Gourlay RN and LeachRH, 1970), and then reported in the United States, Australia, New Zealand, Japan, and Denmark. The bacterium hardly grows on commonly used media and requires special media to grow. It takes 7-14 days to isolate the bacteria in the first generation (the longest period is 4-23 days), and the growth rate of the strain becomes faster after adaptation to the laboratory medium, generally within 2 weeks. The colony morphology of the bacteria was atypical for mycoplasma without a central bulge. Zhang Daoyo...

AI Technical Summary

Problems solved by technology

Growth is demanding on nutrients. In addition to basic nutrition, it is often necessary to add various ingredients such as yeast liquid, in addition to adding 10% to 20% animal serum, and other ingredients, and its configuration is complicated.

At present, the isolation and culture media of Mycoplasma heterogenes are mainly GS medium and modified Friis medium, but there are still problems such as long culture time, low titer of viable bacteria, and poor reproductive ability.

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