Biological product for treating gosling plague and preparing method thereof

A biological product and goose plague technology, applied in biochemical equipment and methods, medical preparations containing active ingredients, microorganisms, etc., can solve the problem of low curative effect of goose plague treatment drugs

Inactive Publication Date: 2016-12-21
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the technical defects of the prior art, and provides a biological product for the treatment of goose plague and its preparation method, so as to solve the technical problem of the low curative effect of the medicine for the treatment of goose plague in the prior art

Method used

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  • Biological product for treating gosling plague and preparing method thereof
  • Biological product for treating gosling plague and preparing method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 (preparation of gosling plague egg yolk antibody IgY)

[0027] (1) Preparation of highly immune eggs: immunize laying hens with gosling plague inactivated vaccine, the immunization dose is 1ml / hen, and the immunization interval is 14 days. Eggs can be collected with a titer higher than 1:64.

[0028] (2) Extraction and preparation of egg yolk antibody IgY: immerse high-immune eggs in 1% bromogeramine solution, soak and sterilize for 15 minutes, and dry for later use; mechanically beat the eggs, separate and collect the egg yolks; emulsify with a colloid mill, add vinegar at a ratio of 1:4 Salt buffer solution (pH5.2), stirred for 60 minutes, filtered with a plate and frame filter; added 2% octanoic acid to the filtered supernatant, stirred vigorously for 60 minutes, and filtered with a plate and frame; the filtered supernatant was sterilized with a 0.22 μm microporous filter .

Embodiment 2

[0029] Embodiment 2 (preparation of gosling plague VP3 protein antigen)

[0030] Using the gene cloning technology, the gosling plague VP3 gene was cloned into the prokaryotic expression vector, transfected into the prokaryotic expression host, and induced to express. Afterwards, the VP3 protein was purified by affinity chromatography. The specific operation method is: use the VP3 co-conserved fragment of the classic strain of goose plague virus in GenBank, use the software Premier 5.0 to design and synthesize the upstream and downstream primers of the VP3 gene; take the liver tissue of goose infected with goose plague virus, grind and homogenate Afterwards, total RNA was extracted by conventional RNA extraction technology and reverse-transcribed into cDNA; using cDNA as a template, the VP3 gene was amplified and recovered by using the upstream and downstream primers of the VP3 gene; the recovered product and the expression vector used used the same restriction endogenous Dic...

Embodiment 3

[0031] Embodiment 3 (titer determination of gosling plague VP3 protein antigen and gosling plague egg yolk antibody)

[0032]Use the agar diffusion test team to determine the titer of gosling plague VP3 protein antigen and gosling plague egg yolk antibody. The specific operation is as follows: prepare an agar plate with 8% NaCl and 1% agarose; use plum blossom holes to punch holes on the agar plate;

[0033] (1) Detection of VP3 protein antigen titer: After doubling dilution of VP3 protein antigen, inject it into the peripheral wells of the plum blossom holes, and add gosling plague standard positive serum in the central well, and the sample volume should be filled without overflowing. Put the agar plate into a wet box and act at 37°C for 24h to 48h. The maximum dilution factor at which precipitation lines appear in the central well and the peripheral well is the antigen titer of the VP3 protein.

[0034] (2) Detection of yolk antibody titer: after doubling dilution of yolk a...

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Abstract

The invention provides a biological product for treating gosling plague and a preparing method thereof. The technical scheme is based on the good controlling effect of an antigen-antibody compound on a virus, a combination scheme with VP3 protein as an antigen and an egg yolk antibody as an antibody is determined according to the characteristics of a gosling plague virus, and on this basis, the specific preparing methods of the VP3 protein and the egg yolk antibody are further designed. According to the technical scheme, on the basis of the original gosling-plague egg yolk antibody, the gosling-plague VP3 protein antigen is added, and a compound is prepared. The gosling-plague antigen-antibody compound can resist persistent infection caused by pathogenic microorganism, excess using of antibiotics is avoided, the interference of a maternal antibody is broken through, and an intense stress reaction caused by live vaccine is reduced; as the used antigen is not a complete virus antigen, but is the antigen prepared through the VP3 protein of the gosling plague, the danger that full viruses are dispersed outwards can be reduced, and the biological product is safer, more reliable and more effective.

Description

technical field [0001] The invention relates to the technical field of biological products, and further relates to the antiviral treatment of compound biological products, in particular to a biological product for treating goose plague and a preparation method thereof. Background technique [0002] Gosling plague (Gosling Plague, GP), also known as goose parvovirus infection (Goose Parvovirus Infection), is an acute or subacute septic infectious disease of goslings caused by goose parvovirus (Goose Parvovirus, GPV). The disease mainly affects young geese and muscovy ducks aged 4 to 20 days. It is characterized by acute enteritis and liver, kidney, and heart parenchymal organ lesions. It has the characteristics of rapid spread, high morbidity and mortality. The disease is one of the most serious infectious diseases that harm the goose industry. It is popular in different degrees every year, causing considerable economic losses and seriously endangering the sustainable, stable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K39/12A61P31/20
CPCA61K39/12A61K39/42A61K2039/552C12N2750/14034A61K2300/00Y02A50/30
Inventor 刘冠星苏建东杨保收付旭彬李守军
Owner TIANJIN RINGPU BIO TECH
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