A kind of method of tissue culture of oil camphor

Abstract

The invention belongs to the technical field of woody plant vegetative propagation and particularly relates to a cinnamomum longepaniculatum vegetative propagation method, in particular to a cinnamomum longepaniculatum tissue culture method. The cinnamomum longepaniculatum tissue culture method comprises the steps of induction culture, propagation culture, rooting culture and seedling hardening. The success rate of cinnamomum longepaniculatum tissue culture can be effectively increased through an improved MS culture medium, designed for cinnamomum longepaniculatum, in culture media for induction culture, propagation culture and rooting culture, a large quantity of cinnamomum longepaniculatum seedlings uniform in cinnamomum longepaniculatum oil content and specification and stable in species nature can be produced in a short period, and a method is provided for quick and high-quality forestation of cinnamomum longepaniculatum.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of woody plants, in particular to a method for asexual propagation of camphor oleifera. Background technique [0002] Cinnamomum oleifera is a precious tree species of the genus Cinnamomum lauraceae, and Cinnamomum oleifera can be used to extract camphor oil, which has broad market prospects. Nowadays, most of the propagation methods of oil camphor are propagated by seeds, cuttings or layering. But because the camphor seed contains a large amount of oil, it is easy to lose the germination ability, causing the success rate of seed propagation to be very low. The yield and quality are not high, affecting the economic benefits after afforestation. And when adopting the method for cutting or layering to propagate, because camphor is a difficult rooting plant, the success rate is not high, and the camphor seedlings grown by cutting or layering grow slowly, have poor tree shape and lower y...

AI Technical Summary

Problems solved by technology

[0003] Nowadays, although many plants are propagated by tissue culture, there is no report on the successful reproduction of oil camphor by tissue culture. The main reasons why oil camphor tissue culture is difficult to succeed are:

[0004] 1. The content of camphor oil such as eucalyptol, myristene, camphor, and abietol contained in camphor leaves is 1 to 2 times that of camphor leaves, which makes the explants easily brown during tissue culture. one of the reasons for the failure of tissue culture;

[0005] 2. There is no suitable tissue culture medium for Cinnamomum oleifera, and conventional MS, MT, White and other mediums have a low success rate of inducing Cinnamomum camphora, especially MS medium is not suitable for initiating culture;

[0006] 3. In addition to the appropriate medium in the tissue culture process of camphor plants, the requirements for light intensity and temperature are extremely strict. Too high or too low will cause the growth of the subculture material to be slow or not grow seriously, and it will also brown. die;

[0007] 4. Since camphor is a difficult rooting plant, it is also difficult to induce rooting of camphor seedlings