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Application of carboxylated multiwalled carbon nanotube to preparation of anti-tumor metastasis medicine

A multi-walled carbon nanotube, anti-tumor metastasis technology, applied in the field of nanomaterials, can solve the problems of low photoelectric conversion efficiency of carbon nanotubes, difficult to improve, etc., and achieves difficult drug resistance, convenient administration, and simple and easy production process. Effect

Inactive Publication Date: 2017-01-04
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the unique photothermal properties of carbon nanotubes can be used to directly kill tumor cells, but the photoelectric conversion efficiency of carbon nanotubes is low and difficult to improve, which is a major bottleneck restricting its therapeutic effect

Method used

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  • Application of carboxylated multiwalled carbon nanotube to preparation of anti-tumor metastasis medicine
  • Application of carboxylated multiwalled carbon nanotube to preparation of anti-tumor metastasis medicine
  • Application of carboxylated multiwalled carbon nanotube to preparation of anti-tumor metastasis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] [Example 1] Inhibitory effect of carboxylated multi-walled carbon nanotubes on tumor metastasis

[0034] (1) Pharmacodynamic experiments in mice

[0035] 1. Preparation of carboxylated multi-walled carbon nanotube suspension

[0036] Dissolve carboxylated multi-walled carbon nanotubes in sodium carboxymethylcellulose solution at a concentration of 1 mg / ml, and ultrasonically vibrate before each use.

[0037] 2. Animal model one

[0038] 1) Inoculation of tumor cells

[0039] Thirty-six six-week-old male C57BL / 6 mice, weighing 18-22g, were injected with 1×10 Lewis lung cancer cells into the tail vein of each mouse on the first day of the experiment. 5 indivual.

[0040] 2) Grouping

[0041] The inoculated mice were randomly divided into 3 groups (model control group, low-dose carbon nanotube group, and high-dose carbon nanotube group), with 12 mice in each group.

[0042] 3) Packet processing

[0043] ① Model control group: 24 hours after inoculation, 80 μl sodium...

Embodiment 2

[0093] [Example 2] Detection of the effect of carboxylated multi-walled carbon nanotubes on the polarization of tumor-associated macrophages

[0094] (1) Immunofluorescence staining

[0095] 1. Take the lung tissue of the Lewis lung cancer model animal in (1), fix it with paraformaldehyde, embed it in conventional paraffin, and slice it at 5 μm.

[0096] 2. Submerge the paraffin sections in antigen retrieval buffer (10 mM Tris, 0.5 mM EGTA aqueous solution, pH9.0), and heat in a microwave oven at 500 W for 20 minutes.

[0097] 3. Paraffin sections were incubated in PBS containing 5% bovine serum albumin (BSA).

[0098] 4. Incubate with primary antibody overnight at 4°C.

[0099] 5. Wash 3 times with PBS, and incubate with secondary antibody for 1 hour at room temperature in the dark.

[0100] 6. Wash 3 times with PBS, add DAPI staining solution dropwise, and incubate at room temperature in the dark for 10 minutes.

[0101] 7. Wash 3 times with PBS, dry the slices slightly,...

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Abstract

The invention discloses new application of a carboxylated multiwalled carbon nanotube to preparation of anti-tumor metastasis medicine. The application is characterized in that the carboxylated multiwalled carbon nanotube can restrain proliferation of tumor cells by targeting tumor microenviroment and reprogramming tumor-associated macrophages (mainly in an M2 phenotype) to be in an M1 phenotype, and is safe and low in toxin for normal cells of a human body. In this way, the carboxylated multiwalled carbon nanotube has the application prospect in preparation of the anti-tumor metastasis medicine.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to the new application of carboxylated multi-walled carbon nanotubes in the preparation of antitumor drugs. Background technique [0002] Tumor is the number one killer threatening human life. According to the World Health Organization survey, nearly 14.1 million cancer patients are newly discovered every year in the world, accounting for 1 / 5 of the total deaths. Tumor metastasis refers to the process in which cancer cells transfer from the original site to other sites and form new lesions. More than 90% of tumor patients die from metastasis, which is the main reason for the difficulty and failure of cancer treatment and the high mortality of cancer patients. Therefore, the prevention and treatment of tumor metastasis has become an urgent and difficult task. [0003] The traditional methods of treating tumor metastasis include radiotherapy and chemotherapy. However,...

Claims

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Application Information

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IPC IPC(8): A61K33/44A61K9/00A61P35/00A61P35/04
CPCA61K9/0092A61K9/10A61K33/44A61K47/38
Inventor 杨静吴练练梁峥琰夏煜琦徐阳
Owner WUHAN UNIV
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