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Double-layer composite hydrogel for repairing bone and cartilage tissues as well as preparation method and application of double-layer composite hydrogel

A composite hydrogel, double-layer composite technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of poor fracture toughness, failure to achieve osteochondral joint repair, and the inability to simulate double-layer materials. The effect of reducing work intensity, simple preparation method, and good repair effect

Active Publication Date: 2017-01-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the repair of joint defects of osteochondral bone, tissue engineering scaffolds such as type I collagen gel, hyaluronic acid gel, and fibrin gel currently used clinically have the following defects: poor mechanical properties; poor self-repair performance; It is not easy to fix and is lost from the defect site; and when using growth factors and proteins to repair osteochondral joints, the interface between bone and cartilage cannot be simulated between the double-layer materials used for osteochondral repair, so that the bone and cartilage cannot be reached. The effect of combined repair
However, the bioceramics of hydroxyapatite, which has been widely studied and applied clinically, is limited in application due to poor mechanical properties, especially poor fracture toughness.
[0007] Therefore, in the field of biomaterials and medicine, the joint repair of osteochondral defects has not been effectively solved, and there is still a great demand for repair materials and methods for joint repair of osteochondral

Method used

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  • Double-layer composite hydrogel for repairing bone and cartilage tissues as well as preparation method and application of double-layer composite hydrogel
  • Double-layer composite hydrogel for repairing bone and cartilage tissues as well as preparation method and application of double-layer composite hydrogel
  • Double-layer composite hydrogel for repairing bone and cartilage tissues as well as preparation method and application of double-layer composite hydrogel

Examples

Experimental program
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Effect test

preparation example Construction

[0048] The present invention also provides a preparation method of the above-mentioned SA / Aker / GDL and SA / AG double-layer composite hydrogel, including the following steps:

[0049] Step 1. Heat the liquid agarose solution and mix it with the sodium alginate solution in a certain ratio for later use;

[0050] Step 2. Prepare a mixed solution of sodium alginate, magnesia feldspar, and glucono lactone in a certain proportion for use;

[0051] Step 3. Add the agarose solution / sodium alginate mixed solution of step 1 into the mold and let stand to condense into a SA / AG composite hydrogel, and add the sodium alginate / magnesia feldspar / gluconolide mixed solution of step 2 into the mold Above the formed SA / AG composite hydrogel, continue to stand and condense to obtain a double-layer composite hydrogel.

[0052] Further, in the preparation method of the double-layer composite hydrogel, the mass volume percentage (g / ml) of agarose and sodium alginate in step 1 are both 2% solutions;

[0053] F...

Embodiment 1

[0069] Example 1. Preparation of SA / Aker / GDL and SA / AG double-layer composite hydrogel

[0070] Step 1. Dissolve 2 g of sodium alginate powder in 100 mL of deionized water with continuous stirring to prepare a 2% medium viscosity sodium alginate solution. The agarose solution heated into a liquid state is prepared into a solution with a mass volume (g / ml) percentage of 2%, and mixed with the sodium alginate solution at a volume ratio of 1:1 to obtain a sodium alginate and agarose mixed solution for use;

[0071] Step 2. Grind and mix 2% SA solution and 2% Aker powder with a mass volume (g / ml) percentage of 2%, and then add GDL powder with a mass volume (g / ml) percentage of 2%, and mix evenly to prepare seaweed The mixed solution of sodium, magnesia feldspar, and gluconolide with a mass volume (g / ml) percentage of 2% for use;

[0072] Step 3. Take about 500 μL of the mixed solution of sodium alginate and agarose from step 1 into the mold and let stand to condense into a SA / AG composi...

Embodiment 2

[0074] Example 2. Analysis of appearance and microstructure of SA / Aker / GDL and SA / AG double-layer composite hydrogels

[0075] The SA / Aker / GDL and SA / AG double-layer hydrogels prepared in Example 1 were freeze-dried, and the hydrogel and its connection interface were observed with an optical microscope.

[0076] Such as figure 2 As shown, the SA / AG layer and SA / Aker / GDL layer of the double-layer composite hydrogel have a tightly bonded connection interface, and the connection transition between the double-layer composite hydrogel is natural.

[0077] The SA / Aker / GDL and SA / AG double-layer hydrogels prepared in Example 1 were freeze-dried, and the dried double-layer composite hydrogel samples were cut to obtain a relatively flat internal cross-section. Scanning electron microscope (SEM) observed the cross-sectional structure of the junction of the double-layer hydrogel under an acceleration voltage of 10kV.

[0078] As attached image 3 As shown, the left side is the SA / AG layer of th...

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Abstract

The invention discloses a double-layer composite hydrogel material applicable to bone and cartilage repair. A bone repair layer consists of a sodium alginate / akermanite / glucolactone composite hydrogel; and a cartilage repair layer consists of a sodium alginate / agarose composite hydrogel. The invention also discloses a preparation method and an application of the double-layer composite hydrogel. The double-layer composite hydrogel disclosed by the invention can simulate bone and cartilage tissues, and interfaces of the double-layer composite hydrogel are closely connected, so that a bone and cartilage bonding interface can be simulated. The double-layer composite hydrogel disclosed by the invention, while promoting the regeneration of the bone and cartilage tissues, can also promote angiogenesis in new bone tissues, so that bone and cartilage tissue repair is promoted.

Description

Technical field [0001] The invention relates to the fields of biomedical materials, tissue engineering and medicine, in particular to a double-layer composite hydrogel material and its preparation method and application. Background technique [0002] Osteochondral injury caused by trauma and osteoarthritis is a common disease in orthopedics. There are no blood vessels, lymphatics and nerves in the articular cartilage, so it lacks the ability to repair itself. Once the damage occurs, it must be repaired or replaced by surgery. Since osteochondral is composed of two different tissues, there are significant differences in composition and performance. Once the osteochondral joint defect is caused, the repair process will be more complicated. [0003] In recent years, certain progress has been made in repairing cartilage by means of tissue engineering. The currently clinically used tissue engineering scaffolds include type I collagen gel, hyaluronic acid gel, and fibrin gel. However, ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/20A61L27/10A61L27/52A61L27/50A61L27/54
CPCA61L27/10A61L27/20A61L27/50A61L27/52A61L27/54A61L2300/252A61L2300/802A61L2430/02A61L2430/06C08L5/04C08L5/12
Inventor 李海燕董昕王晓萌
Owner SHANGHAI JIAO TONG UNIV
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