A double-layer composite hydrogel for repairing osteochondral tissue, preparation method and application

A composite hydrogel, double-layer composite technology, applied in tissue regeneration, prosthesis, medical science and other directions, can solve the problems of poor fracture toughness, poor self-healing performance, inability to simulate double-layer materials, etc., and achieves a simple preparation method. , the effect of reducing work intensity and good repair effect

Active Publication Date: 2019-08-06
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the repair of joint defects of osteochondral bone, tissue engineering scaffolds such as type I collagen gel, hyaluronic acid gel, and fibrin gel currently used clinically have the following defects: poor mechanical properties; poor self-repair performance; It is not easy to fix and is lost from the defect site; and when using growth factors and proteins to repair osteochondral joints, the interface between bone and cartilage cannot be simulated between the double-layer materials used for osteochondral repair, so that the bone and cartilage cannot be reached. The effect of combined repair
However, the bioceramics of hydroxyapatite, which has been widely studied and applied clinically, is limited in application due to poor mechanical properties, especially poor fracture toughness.
[0007] Therefore, in the field of biomaterials and medicine, the joint repair of osteochondral defects has not been effectively solved, and there is still a great demand for repair materials and methods for joint repair of osteochondral

Method used

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  • A double-layer composite hydrogel for repairing osteochondral tissue, preparation method and application
  • A double-layer composite hydrogel for repairing osteochondral tissue, preparation method and application
  • A double-layer composite hydrogel for repairing osteochondral tissue, preparation method and application

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preparation example Construction

[0048] The present invention also provides a preparation method for the above-mentioned SA / Aker / GDL and SA / AG double-layer composite hydrogel, comprising the following steps:

[0049] Step 1. Mix the agarose solution heated to a liquid state with the sodium alginate solution in a certain proportion for use;

[0050] Step 2, sodium alginate, feldspar, and gluconolactone are formulated into a mixed solution in a certain proportion for use;

[0051] Step 3. Add the agarose solution / sodium alginate mixed solution of step 1 into the mold and let it stand to condense to form a SA / AG composite hydrogel, and add the sodium alginate / magnesia feldspar / gluconolactone mixed solution of step 2 into the mold On the top of the formed SA / AG composite hydrogel, continue to stand and condense to obtain a double-layer composite hydrogel.

[0052] Further, in the preparation method of the double-layer composite hydrogel, the mass volume percentage (g / ml) of agarose and sodium alginate in step 1 ...

Embodiment 1

[0069] The preparation of embodiment 1.SA / Aker / GDL and SA / AG bilayer composite hydrogel

[0070] Step 1. Dissolve 2 g of sodium alginate powder in 100 mL of deionized water and keep stirring to prepare a 2% medium viscosity sodium alginate solution. Prepare the agarose solution heated to a liquid state as a solution with a mass volume (g / ml) percentage of 2%, and mix it with the sodium alginate solution at a volume ratio of 1:1 to obtain a mixed solution of sodium alginate and agarose for use;

[0071] Step 2. Grinding and mixing 2% SA solution with 2% Aker powder by mass volume (g / ml) percentage, then adding GDL powder with 2% mass volume (g / ml) percentage, and mixing evenly to prepare seaweed Sodium phosphate, feldspar, gluconolactone mass volume (gram / ml) percentage is the mixed solution of 2% stand-by;

[0072] Step 3. Take about 500 μL of the mixed solution of sodium alginate and agarose in step 1 and add it to the mold and let it stand to condense to form a SA / AG compos...

Embodiment 2

[0074] Example 2.Appearance and microstructure analysis of SA / Aker / GDL and SA / AG bilayer composite hydrogel

[0075] The SA / Aker / GDL and SA / AG double-layer hydrogels prepared in Example 1 were freeze-dried, and the hydrogels and their connection interfaces were observed with an optical microscope.

[0076] Such as figure 2 As shown, the SA / AG layer and the SA / Aker / GDL layer of the bilayer composite hydrogel have a tightly bonded connection interface, and the connection transition between the bilayer composite hydrogel is natural.

[0077] The SA / Aker / GDL and SA / AG double-layer hydrogel prepared in Example 1 were freeze-dried, and the dried double-layer composite hydrogel sample was cut to obtain a relatively flat internal cross section. Scanning electron microscopy (SEM) was used to observe the cross-sectional structure of the bilayer hydrogel junction at an accelerating voltage of 10kV.

[0078] as attached image 3 As shown, the left side is the SA / AG layer of the double...

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Abstract

The invention discloses a double-layer composite hydrogel material applicable to bone and cartilage repair. A bone repair layer consists of a sodium alginate / akermanite / glucolactone composite hydrogel; and a cartilage repair layer consists of a sodium alginate / agarose composite hydrogel. The invention also discloses a preparation method and an application of the double-layer composite hydrogel. The double-layer composite hydrogel disclosed by the invention can simulate bone and cartilage tissues, and interfaces of the double-layer composite hydrogel are closely connected, so that a bone and cartilage bonding interface can be simulated. The double-layer composite hydrogel disclosed by the invention, while promoting the regeneration of the bone and cartilage tissues, can also promote angiogenesis in new bone tissues, so that bone and cartilage tissue repair is promoted.

Description

technical field [0001] The invention relates to the fields of biomedical materials, tissue engineering and medicine, in particular to a double-layer composite hydrogel material and its preparation method and application. Background technique [0002] Osteochondral damage caused by trauma, osteoarthritis, etc. is a relatively common disease in orthopedics. Articular cartilage lacks self-repair ability due to lack of distribution of blood vessels, lymph and nerves. Once damage occurs, surgical repair or replacement is necessary. Osteochondral is two different tissue structures with significant differences in composition and performance. Once the joint defect of osteochondral is damaged, the repair process will be more complicated. [0003] In recent years, some progress has been made in repairing cartilage by means of tissue engineering. Currently, tissue engineering scaffolds for clinical application include type I collagen gel, hyaluronic acid gel, and fibrin gel. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/10A61L27/52A61L27/50A61L27/54
CPCA61L27/10A61L27/20A61L27/50A61L27/52A61L27/54A61L2300/252A61L2300/802A61L2430/02A61L2430/06C08L5/04C08L5/12
Inventor 李海燕董昕王晓萌
Owner SHANGHAI JIAO TONG UNIV
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