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A kind of alginic acid/pedot conductive porous support and preparation method thereof

A technology of porous scaffold and alginic acid, which is applied in prosthesis, tissue regeneration, medical science, etc., can solve the problems of poor ability to form hydrogel, poor operability, and complicated preparation process, so as to reduce the reduction of biocompatibility , protein adsorption ability enhancement, and the effect of promoting cardiomyocyte differentiation

Active Publication Date: 2019-03-22
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor solubility of conductive polymers (polypyrrole and polythiophene are insoluble in water and most organic solvents), their ability to form hydrogels is poor, and their biodegradability and compatibility with myocardial repair are still poor. It needs to be further improved, so its application in myocardial tissue engineering is limited
In order to improve these shortcomings, researchers used a two-step method to prepare a double-network conductive hydrogel. This type of hydrogel has excellent conductive properties, but its preparation process is complicated. More importantly, the conductive polymer is in the double-network water The distribution in the gel is uneven and the content is not easy to control, and the operability is poor

Method used

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  • A kind of alginic acid/pedot conductive porous support and preparation method thereof
  • A kind of alginic acid/pedot conductive porous support and preparation method thereof
  • A kind of alginic acid/pedot conductive porous support and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Preparation of porous scaffolds with PEDOT / alginic acid ratio of 5:5

[0022] (1) Weigh 50 mg of sodium alginate, dissolve in 1 ml of N-morpholinoethanesulfonic acid-sodium chloride aqueous solution, fully dissolve, and set aside.

[0023] (2) Add 50 mg of 3,4-ethylenedioxythiophene (EDOT) monomer to the sodium alginate solution prepared in step (1), and fully mix with a vortex instrument to obtain an emulsion of EDOT / sodium alginate, spare.

[0024] (3) According to the carboxyl content of sodium alginate, 20.2 mg of adipate dihydrazide (ADH) was added to the mixed solution prepared in step (2). After fully dissolving, 44.3mg of EDC, 11.3mg of NHS and 100mg of ammonium persulfate were sequentially added thereto. After fully mixing by vortexing, the mixed solution was poured into a tetrafluoroethylene mold, and after standing at room temperature for 1 hour, the alginic acid / PEDOT hydrogel was formed.

[0025] (4) Soak the hydrogel prepared in step (3) in ...

Embodiment 2

[0027] Example 2 Preparation of porous scaffolds with PEDOT / alginic acid ratio of 7:3

[0028] (1) Weigh 30 mg of sodium alginate, dissolve in 1 ml of N-morpholinoethanesulfonic acid-sodium chloride aqueous solution, fully dissolve, and set aside.

[0029] (2) Add 70 mg of 3,4-ethylenedioxythiophene (EDOT) monomer to the sodium alginate solution prepared in step (1), and fully mix with a vortex instrument to obtain an emulsion of EDOT / sodium alginate, spare.

[0030] (3) According to the carboxyl content of sodium alginate, 12.1 mg of adipate dihydrazide (ADH) was added to the mixed solution prepared in step (2). After fully dissolving, 26.6 mg of EDC, 7.98 mg of NHS and 140 mg of ammonium persulfate were sequentially added thereto. After fully mixing by vortexing, the mixed solution was poured into a tetrafluoroethylene mold, and after standing at room temperature for 1 hour, the alginic acid / PEDOT hydrogel was formed.

[0031] (4) Soak the hydrogel prepared in step (3) ...

Embodiment 3

[0033] Example 3 Biocompatibility Experiment of Conductive Porous Scaffold Materials

[0034] (1) Cut the stent into thin slices with a diameter of 10 mm and a thickness of 2 mm, sterilize with Co60 radiation, and set aside.

[0035] (2) will 6×10 5 Brown adipose stem cell BADSCs were planted in porous scaffolds at 37°C, 5% CO 2 conditions, 1, 4, 7 and 10 days, adding 10% The phenol red-free medium of the staining solution was incubated for 12 hours, and the empty well plate was set to add The dye solution was the control group. Aspirate the dye solution and detect its absorbance at 570nm and 610nm with a microplate reader. Calculate the reduction rate to characterize the cell viability (such as figure 2 shown).

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Abstract

The invention discloses an alginic acid / PEDOT (poly(3,4-ethylenedioxythiophene)) conductive porous stent and a method for preparing the same. Stent materials for the alginic acid / PEDOT conductive porous stent include covalently cross-linked alginic acid which is used as a substrate, poly-3,4-ethylenedioxythiophene granules are further formed in the substrate by means of in-situ polymerization, and accordingly the alginic acid / PEDOT conductive porous stent has conductive characteristic. The alginic acid / PEDOT conductive porous stent and the method have the advantages that the conductive porous stent materials can be prepared by means of one-step forming, the method is simple, and the alginic acid / PEDOT conductive porous stent contains homogenous and controllable components; the formed stent materials have excellent conductive characteristics and controllable mechanical properties, and the protein adsorption capacity of the alginic acid / PEDOT conductive porous stent with the PEDOT components can be obviously improved; more importantly, the porous stent materials prepared by the aid of the method are excellent in biocompatibility, adhesion and growth of diversified cells can be supported, the myocardial cell differentiation capacity of stem cells can be regulated, and accordingly the alginic acid / PEDOT conductive porous stent and the method have potential application prospects in clinical transformation for myocardial infarction treatment by myocardial tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biological material preparation. Specifically relates to an alginic acid / PEDOT conductive porous support and a preparation method thereof. Background technique [0002] Cardiovascular disease is one of the major diseases that endanger human health and life. Among them, myocardial infarction is the most common. The incidence of myocardial infarction in my country has increased significantly and is showing a younger trend. Recent studies have shown that myocardial tissue engineering based on biomaterials, seed cells and growth factors can repair damaged myocardium and restore heart function to a certain extent, and has become a potential new method for the treatment of myocardial infarction. Among them, biomaterials play a very critical role in providing the necessary mechanical support for the repair of damaged myocardium, and providing a suitable microenvironment for the survival and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/20A61L27/18A61L27/50A61L27/52A61L27/56
CPCA61L27/48A61L27/50A61L27/52A61L27/56A61L2430/20C08L5/04C08L65/00
Inventor 王常勇李俊杰杨伯光郝彤
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA