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Method for preparing marine organism organic liquid fertilizer through composite microbial fermentation

A technology of compound microorganisms and marine organisms is applied in the field of preparing marine bio-organic liquid fertilizers, which can solve the problems of difficult to meet the requirements of industrial production requiring stable and controllable standards, difficult to guarantee product quality, poor controllability of fermentation processes, etc. Yield and resistance, improved production efficiency, effects of high viability and reproduction

Inactive Publication Date: 2017-01-04
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are relatively few studies on the use of microbial fermentation to produce seaweed fertilizers. There are only a few studies that use common microorganisms to perform composting or aerobic fermentation on seaweed raw materials. It is difficult to meet the unified, stable and controllable standards required by industrial production, and it is difficult to guarantee the quality of products

Method used

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  • Method for preparing marine organism organic liquid fertilizer through composite microbial fermentation
  • Method for preparing marine organism organic liquid fertilizer through composite microbial fermentation
  • Method for preparing marine organism organic liquid fertilizer through composite microbial fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1) Preparation of product A: Wash fresh copper algae and brown algae (mass ratio 1:1), remove impurities, soak for 24 hours, grind, transfer to a fermenter, sterilize at 115°C for 30min, and cool to 25°C. ℃ to get the product A, set aside.

[0034] 2) Prepare seed solution: activate and expand Bacillus cereus to prepare Bacillus cereus seed solution X; activate and expand Bacillus saccharophilus to prepare Bacillus saccharophilus seed solution Y; activate Bacillus amyloliquefaciens and expansion culture to prepare Bacillus amyloliquefaciens seed solution Z;

[0035] The medium formula of described Bacillus cereus activation and expansion cultivation is: soybean meal powder 20g, yeast extract 6g, sodium alginate 1.2g, bovine bile salt 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, Magnesium sulfate 0.2g, mannitol 9.5g, water 1000mL, adjust pH 6.8. The expansion condition is: culture at 33°C for 20h.

[0036] The formula of the culture medium for th...

Embodiment 2

[0046] 1) Preparation of product A: Wash and remove fresh kelp and sargassum (mass ratio: 1:1), soak for 36 hours, refine, transfer to a fermenter, sterilize at 125°C for 30 minutes, and cool to Product A was obtained at 30°C and set aside.

[0047] 2) Preparation of seed liquid: the operation method is the same as in Example 1.

[0048] 3) Preparation of product B: inoculate seed liquid X into product A, the inoculum size is 15%, the fermentation temperature is 35°C, the pH value of the reaction is adjusted with oxalic acid and sodium acetate to 7.0, the dissolved oxygen is controlled at 30%, and the rotation speed is 200r / min. The ventilation rate is 1000L / min, the pressure of the fermenter should be maintained at 0.05Mpa, the fermentation time is 16h, and the product B is obtained.

[0049] 4) Preparation of product C: adjust the pH of product B to 4.0, cool the temperature in the fermenter to 3°C, store for 4 days, and then return the temperature to 25°C to obtain product...

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Abstract

The invention relates to a method for preparing a marine organism organic liquid fertilizer through composite microbial fermentation. The method comprises the following steps of firstly thoroughly cleaning fresh marine algae plants, performing impurity elimination, performing soaking, performing defibrination, then respectively preparing strain seed fluid, and inoculating the seed fluid to seaweed bottom materials many times so as to realize classified fermentation. When a composite classified fermentation technique is adopted by the method disclosed by the invention, effects are obviously better than those of single strain fermentation and those of general multi-strain mixed fermentation, besides, the fermentation efficiency is high, the time is short, and the whole technology is high in controllability, green, energy-saving and environmental-friendly. The effective viable count of the marine organism organic liquid fertilizer prepared by the technique is 15 hundred million CFU / ml or above, the pH value of the marine organism organic liquid fertilizer is 6.5-7.0, and the content ratio of N to P to K is (1-2) to 1 to (0.8-1.5).

Description

technical field [0001] The invention belongs to the field of agricultural fertilizers, and in particular relates to a method for preparing marine biological organic liquid fertilizers through a compound microbial fermentation technology. Background technique [0002] At present, the production methods of marine biological organic liquid fertilizer mainly include chemical method, physical method and biological method. The chemical method is mainly to treat seaweed cells with acid, alkali and organic solvents, so that the cells can digest and release endogenous substances. This method is simple to operate and easy to implement, and it is also the method adopted by most seaweed fertilizer manufacturers at home and abroad. However, chemical reagents have considerable damage to the active substance components in seaweed cells, and the residual organic reagents are also potential hazards to the environment. The principle of the physical method is to control the physical environme...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00C12N1/20C12R1/07C12R1/085
CPCC05B7/00C05F11/00C05G3/00C12N1/20C05F17/50C05C3/00C05C9/00C05D1/02C05F9/04C05F11/08Y02W30/40
Inventor 王学江张志凯
Owner WUZHOUFENG AGRI SCI & TECH
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