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Device and method for continuously detecting growth curve of bacteria

A technology of bacteria and detection unit, applied in the field of microbial detection, can solve the problems of inability to measure for a long time, poor controllability of measurement time, and inability to measure the OD value of bacteria in time, and achieves the effect of high accuracy of measurement results, saving time and manpower

Active Publication Date: 2017-01-04
承启医学(深圳)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. It is easy to introduce other bacterial contamination during sampling, causing the experiment to fail;
[0007] 2. Sampling is very time-consuming and labor-intensive;
[0008] 3. The controllability of the measurement time is poor: manual measurement cannot be performed for a long time, such as more than 24 hours; at the same time, since it takes at least 10-20 minutes to measure a sample, it is impossible to measure the OD value of bacteria in a very short time interval;
[0009] 4. Conditions have an impact on the results, and the repeatability of the experiment is not good: when there is a temperature difference between room temperature and bacterial culture temperature, sampling will have a great impact on bacterial growth, and the bacterial sample will be affected, and the accurate value of the bacterial growth detection of the sample will also be affected. influences
[0010] 5. For bacteria that need to be cultured under special conditions, such as anaerobic bacteria or high temperature conditions, it is also impossible to manually measure the growth status of bacteria

Method used

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  • Device and method for continuously detecting growth curve of bacteria
  • Device and method for continuously detecting growth curve of bacteria
  • Device and method for continuously detecting growth curve of bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: the mensuration of bacterial OD value

[0062] The OD value of bacteria refers to the difference in absorbance, the difference between the absorbance of the sample and the absorbance of the reference sample. Absorbance refers to the logarithm to the base 10 of the ratio of the incident light intensity before the light passes through a solution or a substance to the transmitted light intensity after the light passes through the solution or substance. The principle used is Lambert Beer's law (Bouguer- Lambert-Beer law):

[0063]

[0064] Wherein, A: absorbance; k: light absorptance; d: optical path, bacterial culture optical glassware thickness (liquid layer thickness); C: sample concentration; I 0 : initial transmitted light intensity; I: transmitted light intensity. The calculation method of OD value is:

[0065]

[0066]

[0067] Among them, A t0 : Absorbance of pure medium; A t : the absorbance of bacterial liquid after inoculating bacteriu...

Embodiment 2

[0069] Embodiment 2: the measuring device of bacterial OD value

[0070] A device for continuously detecting the concentration or growth state of bacteria, comprising: a detection unit module 1, which is used to detect the part of the solution area that contains or does not contain bacteria, and generates a measurement value; sensor data lines and / or laser control lines 2, used to For data transmission; single-chip microcomputer control platform 3, used to control one or more detection unit modules 1, and record the measured values ​​generated; the first wireless data communication module 4, used for data information output; the second wireless data communication module 5, for receiving the data information of the first wireless data communication module 4; and a terminal data processing device 6, such as figure 1 shown.

[0071] Wherein, the detection unit module 1 includes: a laser 8, which is used as a light source; a digital light intensity detection sensor 9, which is us...

Embodiment 4

[0075] Embodiment 4: Evaluation of the accuracy of the OD value detection device and method of bacterial bacterial liquid

[0076] Control: measure the OD value of the sample using a spectrophotometer, a commonly used bacterial concentration measuring instrument in the laboratory;

[0077] Experimental group: the OD value of the same sample was measured using the device and method for detecting the OD value of the bacterial liquid of the present invention.

[0078] By detecting the control group and the experimental group, measuring a series of samples with different OD values, and performing correlation analysis on the obtained results, the accuracy of the measurement results of the present invention and the correspondence with the values ​​measured by traditional precision instruments are evaluated.

[0079] The specific experimental method includes the following steps:

[0080]1. Cultivate Escherichia coli E.coli BL21(DE3) overnight, dilute it with medium at 12 different d...

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Abstract

The invention relates to a device and method for continuously detecting the concentration or growth state of bacteria. The device comprises a detection unit module, a sensor data line and / or a laser control line, a single chip microcomputer control platform, a wireless data communication module and terminal data processing equipment. According to the device and method, the concentration of the bacteria can be continuously measured in a non-interference state under other special culture conditions, and then the growth state of the bacterial is dynamically monitored and studied in real time.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a measuring device and method for bacterial concentration and growth state. Background technique [0002] When the bacteria are cultured under suitable environmental conditions, take the culture time as the abscissa and the change in the number of bacteria as the ordinate, and make a response to the law of the change in the number of bacteria during the culture period according to the relationship between the change in the number of bacteria and the change in the corresponding time. This type of curve is called a growth curve. It reflects the group growth law of unicellular microorganisms in liquid culture under certain environmental conditions. [0003] Using a spectrophotometer, use 500-600nm light waves to pass through the bacterial liquid in the cuvette, and measure the transmitted beam intensity of the beam. Beer-Lambert Law (Beer-Lambert Law) believes that the propo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/03G01N21/59
Inventor 钟嘉泳张弓杜高飞金静洁
Owner 承启医学(深圳)科技有限公司
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