Method for detecting the concentration of d-tryptophan in solution
A tryptophan, D-type technology, applied in the direction of material electrochemical variables, can solve the problems of high detection limit, low accuracy, low sensitivity, etc., to reduce detection cost, simple and convenient operation, improve sensitivity and accuracy degree of effect
Active Publication Date: 2019-06-07
SUZHOU ZHILVE INTELLECTUAL PROPERTY OPERATION CO LTD
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Problems solved by technology
[0004] Existing methods for detecting amino acids with different configurations include cyclic voltammetry and AC impedance. These methods only use one of the indicators as a basis for simultaneous qualitative and quantitative analysis. For example, cyclic voltammetry only uses the magnitude of the current signal as a method to distinguish different configurations. Amino acids, the AC impedance is distinguished by the resistance value, its accuracy is low, the sensitivity is low, and the detection limit is high
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Embodiment 1
[0046] Prepare the working electrode: immerse the reference electrode, auxiliary electrode and glassy carbon electrode in a mixed solution containing 2 mg / mL of aminated graphene quantum dots and 2 mg / mL of β-cyclodextrin, and take them out after scanning by cyclic voltammetry drying to obtain the modified glassy carbon electrode;
[0047] Among them, the scanning parameters are set as: initial potential 0V, highest potential 1V, lowest point 0V, final potential 0V, scan rate 0.1V / s, scan times 100 times, sensitivity 10 -4 A / V, waiting time is 2s.
[0048] Working electrode pretreatment: place the modified glassy carbon electrode on a polishing cloth containing polishing powder with a particle size of 0.3 μm and polish it to the mirror surface, and then place the glassy carbon electrode in methanol and H at a concentration of 0.5 mol / L in sequence. 2 SO 4 The solution and ultrapure water were sonicated for 30 s, and rinsed with ultrapure water for 1 min after each sonication...
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Abstract
The invention discloses a method for detecting the D-type tryptophan concentration in a solution. A tri-electrode system is used for detecting D-type tryptophan in a to-be-detected solution through differential pulse voltammetry; the D-type tryptophan concentration of the to-be-detected solution is obtained through a linear equation; and the working electrode in the tri-electrode system is a modified glassy carbon electrode that is a glassy carbon electrode modified by aminated graphene quantum dots and beta cyclodextrin. The method allows the D-type tryptophan concentration to be fast detected and is high in sensitivity and accuracy. A related sensing interface is constructed as a tri-electrode system sensor for detection. The potential is adopted for nature determination of tryptophan configuration, and the peak current value is adopted for amount determination. The sensitivity and accuracy of D-type tryptophan detection is improved, and the D-type tryptophan with the concentration lowest to 9.3*10<-8> mol / L can be detected. According to the method, detection cost is greatly reduced, and the operation is simple and convenient.
Description
technical field [0001] The invention relates to the technical field of isomeric amino acid detection. More specifically, the present invention relates to a method for detecting the concentration of D-type tryptophan in a solution by differential pulse voltammetry based on the electrochemical sensing of aminated graphene quantum dots and β-cyclodextrin modification. technical background [0002] Tryptophan is an important precursor substance for auxin biosynthesis in plants. Its structure is similar to indole acetic acid, and it is ubiquitous in higher plants. Auxin can be synthesized from tryptophan. This product is an important nutritional agent. It can participate in the renewal of plasma proteins in animals, and can promote riboflavin to play a role. It can also help the synthesis of niacin and heme. It can significantly increase the antibodies in the fetuses of pregnant animals, and it can promote lactation for lactating cows and sows. effect. When livestock and poul...
Claims
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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 黄珊肖琦卢双燕
Owner SUZHOU ZHILVE INTELLECTUAL PROPERTY OPERATION CO LTD