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Reagent and method for quantitatively detecting serum amyloid proteins A in whole blood

A technology for quantitative detection of serum starch, applied in biological testing, preparation of test samples, measuring devices, etc., can solve the problems of increasing sample demand, time-consuming and laborious, and increasing operation steps, so as to meet the requirements of clinical testing and improve The effect of high sensitivity and detection sensitivity

Active Publication Date: 2017-01-04
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all use serum or plasma as the test sample, and all blood needs to be pre-separated from red blood cells before testing, requiring special separation devices (such as centrifuges, etc.), increasing the operation steps, time-consuming and laborious, and increasing the demand for samples. , for children and newborns and patients with extensive burns, it has always been a difficult problem to collect enough blood samples for testing
There are currently no kits or assays capable of detecting SAA in whole blood

Method used

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  • Reagent and method for quantitatively detecting serum amyloid proteins A in whole blood
  • Reagent and method for quantitatively detecting serum amyloid proteins A in whole blood
  • Reagent and method for quantitatively detecting serum amyloid proteins A in whole blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Quantitative detection reagents for serum amyloid A in whole blood, including reagent R1 and reagent R2.

[0043] Specifically:

[0044] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the cell-breaking component is 0.10% fatty acid salt by mass percentage, the buffer solution is a glycine buffer solution with a pH value of 6.0 and a concentration of 10mmol / L, the surfactant is Tween20 with a mass percentage of 0.01%, and the electrolyte is mass percent The sodium salt is 3.00%, the preservative is sodium azide with a mass percentage of 0.01%, the reaction accelerator is polyethylene glycol 2000 with a mass percentage of 0.01%, and the stabilizer is bovine serum albumin with a mass percentage of 0.01%.

[0045] Reagent R2 includes: latex particles, buffer, surfactant, electrolyte, preservative, and stabilizer. Wherein: the percentage composition of latex particle is 0.10%, is ...

Embodiment 2

[0051] Quantitative detection reagents for serum amyloid A in whole blood, including reagent R1 and reagent R2. Specifically:

[0052] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the cell-breaking component is 2.00% by mass percentage of sulfate ester salt, the buffer is a Tris buffer solution with a pH value of 8.0 and a concentration of 100mmol / L, the surfactant is Tween80 with a mass percentage of 3.00%, and the electrolyte is mass % The percentage is 0.10% of potassium salt, the preservative is 2.00% by mass of sorbate, the reaction accelerator is 4.00% by mass of polyethylene glycol 4000, and the stabilizer is 3.00% by mass of gelatin.

[0053] Reagent R2 includes: latex particles, buffer, surfactant, electrolyte, preservative, and stabilizer. Wherein: the percentage composition of latex particle is 0.80%, is coated with SAA antibody, and particle size is 300nm, and buffer s...

Embodiment 3

[0059] Quantitative detection reagents for serum amyloid A in whole blood, including reagent R1 and reagent R2. Specifically:

[0060] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the broken cell component is the phosphate ester salt of 0.50% by mass percentage, the buffer solution is the phosphate buffer solution with a pH value of 7.0 and a concentration of 50mmol / L, the surfactant is Span20 with 1.00% by mass percentage, and the electrolyte is the mass percent Percentage is 2.00% calcium salt, preservative is benzoic acid and its salts that are 0.05% by mass percentage, reaction accelerator is polyethylene glycol 8000 that is 2.00% by mass percentage, and stabilizer is ethanol that is 0.10% by mass percentage .

[0061] Reagent R2 includes: latex particles, buffer, surfactant, electrolyte, preservative, and stabilizer. Wherein: the percentage composition of latex particle is 0...

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Abstract

The invention discloses a reagent for quantitatively detecting serum amyloid proteins A in whole blood. The reagent comprises reagents R1 and reagents R2. The reagents R1 comprise disruption components, buffer solution, surfactants, electrolyte, preservatives, reaction promoters and stabilizers; the reagents R2 comprise latex particles, surfactants, electrolyte, preservatives, stabilizers and buffer solution; the disruption components are used for removing measurement interference red blood cells, blood cells are not separated by the disruption components, and the original whole blood can be used; anti-human SAA antibodies are coated by the latex particles, and the particle sizes of the latex particles are 50-300 nm. The reagent has the advantages that a detection sample can be the whole blood such as peripheral blood, requirements on high sensitivity and wide linear ranges can be simultaneously met, the reagent is excellent in anti-interference performance, accuracy and precision, and clinical examination requirements can be met. The invention further discloses a method for quantitatively detecting the serum amyloid proteins A in the whole blood.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a quantitative detection reagent for serum amyloid A in whole blood. The invention also relates to a quantitative detection method for serum amyloid A in whole blood. Background technique [0002] Serum amyloid A (serum amyloid A protein, SAA) is an acute phase reaction protein, which can rise rapidly within 48-72 hours of inflammation or disease infection, and increase to about 1000 times the initial concentration, and Rapid decline in the recovery period of the disease (Urieli Shoval S, et, al. Expression and function of serum amyloidA, a major acute phase protein, in normal and disease states[J].Curr OpinHematol, 2000, 7(1):64-69. ). In infectious diseases, the combined detection of SAA and CRP helps to distinguish between bacterial infection and viral infection (SAA is elevated in both viral and bacterial infections, while CRP is hardly or not significantly eleva...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31G01N1/28G01N1/38
CPCG01N1/28G01N1/38G01N21/31G01N33/68G01N2021/3185
Inventor 王剑林清菁范翠翠周金庆
Owner PUREBIO LAB NINGBO
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