Blood type detection card based on microporous membranes and blood type detection system

A technology of detection system and microporous membrane, which is applied in blood type detection system and blood type detection card field to achieve the effects of low cost, convenience for medical operators, and improvement of work efficiency

Inactive Publication Date: 2017-01-04
中山生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the optimal microcolumn gel blood type test card can only be stored at 2-25°C for 1 year

Method used

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  • Blood type detection card based on microporous membranes and blood type detection system
  • Blood type detection card based on microporous membranes and blood type detection system
  • Blood type detection card based on microporous membranes and blood type detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] [Example 1] Preparation of test card

[0057] Step 1. A processing method for a microporous membrane, comprising the following steps:

[0058] (1) Basement film imaging: polyethersulfone, nylon, polypropylene, polyethylene, polyethylene terephthalate, polycarbonate, polytetrafluoroethylene, polyvinylidene fluoride with a thickness of 6-50 μm can be used , polytetrafluoroethylene dispersion resin, polyurethane, polyvinyl chloride, polyimide, glass fiber membrane, mixed cellulose, organic silicon membrane is a base membrane;

[0059] (2) Irradiation: choose a heavy ion beam with an energy of 1-500 MeV and a current intensity of 0.1 nanoampere-10 microamperes to irradiate the basement membrane from one side, and the irradiation time is 0.1 seconds to nine hours;

[0060] (3) Sensitization: Utilize an ultraviolet lamp with a wavelength of 200-365nm to irradiate the irradiated basement film, the power of the ultraviolet lamp is 50-2000 watts, the distance between the lamp a...

Embodiment 2

[0065] [Example 2] Preparation of detection card supporting reagents: anti-A reagent, anti-B reagent, anti-D reagent, anti-human IgG reagent, anti-C3d reagent, anti-human IgG+C3d reagent, anti-A1 reagent, anti-C reagent, anti-c Reagent, Anti-E Reagent, Anti-E Reagent

[0066] (1) Preparation of Antibody Diluent

[0067] Weigh Na 2 HPO 4 12H 2 O 0.715g, KH 2 PO 4 0.408g, NaCl 1.75g, EDTA-2Na 2.0g, BSA10g, PEG6000 0.5g, sucrose 40g and NaN 3 0.1g, fully dissolved in purified water to 1000ml, and stored at 2-25°C.

[0068] (2) Selection of antibodies

[0069] Anti-A monoclonal antibody titer ≥ 128.

[0070] Anti-B monoclonal antibody titer ≥128.

[0071] Anti-D monoclonal antibody titer ≥64.

[0072] Anti-human IgG antibody titer ≥ 50.

[0073] Anti-C3d antibody titer ≥ 50.

[0074] Anti-human IgG+C3d antibody titer ≥50.

[0075] Anti-A1 antibody titer ≥ 50.

[0076] Anti-C antibody titer ≥50.

[0077] Anti-c antibody titer ≥ 50.

[0078] Anti-E antibody titer ≥...

Embodiment 3

[0092] [Example 3] Positive typing experiment

[0093] (1) Label the test card, and every 4 microcolumn tubes are 1 serving. Set 1-4 microcolumn tubes as tube A, tube B, tube D, and tube Ctl. respectively. Each test card can detect 2 people.

[0094] (2) Add anti-A reagent to tube A, anti-B reagent to tube B, anti-D reagent to tube D, and antibody diluent to tube Ctl, 50 μl per tube.

[0095] (3) Add 4% erythrocyte suspension diluted with normal saline to 1-4 microcolumn tubes successively, 10 μl per tube.

[0096] (4) Put the card into a medical centrifuge and centrifuge at 800rpm for 2 minutes, 1500rpm for 3 minutes, and take out the visual judgment result.

[0097] (5) Result judgment

[0098] Red blood cells agglutinate on the surface of the microporous membrane, which is a positive result;

[0099] Red blood cells condense at the bottom of the microcolumn tube, which is a negative result;

[0100] The results of blood type determination are shown in the table below:...

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Abstract

The invention provides a blood type detection card based on microporous membranes. The blood type detection card comprises a plurality of micro-column tubes and the microporous membranes fixed in the middles of the micro-column tubes; the micro-column tubes are used for accommodating erythrocytes to be detected and a detection reagent containing an antibody; the pore diameters of the microporous membranes are 0.1 [mu]m to 10 [mu]m; the pore densities of the microporous membranes are 105 to 108 per cm<2>. According to the requirement of blood type detection, by selecting the pore diameters of the microporous membranes, erythrocytes which are not combined with the antibody settle to the bottoms of the micro-column tubes via the microporous membranes, while erythrocytes which are combined with the antibody are blocked on the microporous membranes, and the blood type is judged according to the red position of the erythrocytes. The blood type detection card provided by the invention has the characteristics that the guarantee period is long; the cost is low; the blood type detection card can be used for detection in almost all blood type related identification tests.

Description

technical field [0001] The invention relates to the field of blood type testing, in particular to a blood type testing card and a blood type testing system. Background technique [0002] Blood type is a method of classifying blood, usually referring to the typing of red blood cells, based on whether there are certain heritable antigenic substances on the surface of red blood cells. Antigenic substances can be proteins, carbohydrates, glycoproteins or glycolipids. Usually some antigens come from the alleles of the same gene or the coding products of several closely linked genes, and these antigens form a blood group system. There are 30 blood group systems that have been discovered in humans and recognized by the International Society of Blood Transfusion, such as ABO blood group system, Rh blood group system, MNS blood group system, P blood group system, etc., among which the ABO blood group system and Rh blood group system are the most important. At present, the most comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 宋小冬王胜岚周征宇崔清臣张家骥李思哲李峰
Owner 中山生物工程有限公司
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