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Application of sodium alginate oligose and derivative to treatment of inflammations

A technology of algin oligosaccharides and derivatives, which is applied in the direction of medical preparations containing active ingredients, anti-inflammatory agents, drug combinations, etc., and can solve the problems of drug application limitations and large molecular weight of alginate

Inactive Publication Date: 2017-01-25
GREEN VALLEY (SHANGHAI) PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the large molecular weight of alginate, its application in medicine is limited to a certain extent.

Method used

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  • Application of sodium alginate oligose and derivative to treatment of inflammations
  • Application of sodium alginate oligose and derivative to treatment of inflammations
  • Application of sodium alginate oligose and derivative to treatment of inflammations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Alginate Oligosaccharides

[0046] Weigh 1 g of polymannuronic acid sodium salt (weight average molecular weight 8235 Da, provided by Shanghai Green Valley Pharmaceutical Co., Ltd.), and add appropriate amount of distilled water to prepare a 1% (weight percent) aqueous solution of polymannuronate sodium. The pH value of the 1% aqueous sodium polymannuronate solution was adjusted to 4 with hydrochloric acid, and then the aqueous solution was placed in an autoclave. The reaction was heated at 110°C for 4 hours. The reacted solution was taken out from the autoclave and allowed to cool. After cooling, the pH value of the reacted solution was adjusted with NaOH solution to obtain a neutral liquid. Under the condition of stirring, the neutral liquid was slowly added into ethanol in an amount 4 times the volume of the liquid, alcohol precipitation was carried out and the mixture was allowed to stand overnight. The solid matter obtained by alcohol pr...

Embodiment 2

[0048] Example 2 Preparation of algin oligosaccharide oxygen hydrolysis product

[0049] Take 5 g of the above-mentioned crude alginate oligosaccharides that have not been separated by molecular exclusion chromatography to prepare a 5% (weight percent) aqueous solution. Fresh oxidant copper hydroxide was prepared by adding 25 ml of 5% (weight percent) copper sulfate solution to 50 ml of 10% (weight percent) sodium hydroxide solution and mixing immediately. The fresh oxidant copper hydroxide was immediately added to 40ml of the above-mentioned 5% (weight percent) alginate oligosaccharide solution, and heated in a boiling water bath until no brick-red precipitate was produced. The reaction system was centrifuged to remove precipitates to obtain a supernatant. Get a little supernatant and add the oxidizing agent again to check whether there is brick red precipitate. If there is still a brick-red precipitate, then continue to react all the supernatant obtained by the centrifugat...

Embodiment 3

[0051] Example 3 Effect of Alginate Oligosaccharide Derivatives on Inflammation in Mice Induced by Xylene

[0052] 1 Experimental materials

[0053] 1.1 Drugs and reagents

[0054] According to the method of Example 2, a mixture of alginic oligosaccharides (n=2-8, ie components 4-10 in the figure) of formula (II) was prepared. The mixture is off-white powder, soluble in water; aspirin, produced by Shijiazhuang Ouyi Pharmaceutical Co., Ltd.; xylene is a domestic analytical reagent.

[0055] 1.2 Experimental animals

[0056] Kunming mice, male, weighing 21±1.5g, were provided by Shanghai Sipro-Bikay Experimental Animal Co., Ltd.

[0057] 1.3 Experimental Instruments

[0058]Electronic analytical balance, Beijing Sartorius Instrument System Co., Ltd.

[0059] 2 Experimental methods

[0060] 2.1 Animal grouping and administration

[0061] Xylene is a non-specific inflammatory factor that can locally cause acute inflammation with increased vascular permeability and leukocyte...

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Abstract

The invention relates to application of sodium alginate oligose and a derivative to treatment of inflammations. The sodium alginate oligose and the derivative of the sodium alginate oligose are particularly mannuronic acid oligose or mannuronic acid oligose with a carboxyl at a 1-position of a reducing end and the derivative or a pharmaceutically acceptable salt, formed by connecting a beta-D-mannuronic acid through a 1,4-glucosidic bond. The inflammations are particularly a vascular inflammation, a neuroinflammation, a nervous centralis inflammation, arthritis, Crohn's disease, ulcerative colitis, inflammatory diabetic ulcer, psoriasis, atopic dermatitis and eczema.

Description

technical field [0001] The invention relates to algin oligosaccharides and derivatives thereof and their application in treating inflammation. Background technique [0002] Inflammation refers to the physiological response of biological tissues stimulated by trauma, hemorrhage, pathogenic infection, foreign bodies and other stimuli. The inflammatory response involves changes in some specific autologous active substances, such as prostaglandins and leukotrienes, and specific inflammatory cytokines, such as interleukins. The occurrence of inflammation is in addition to removing foreign bodies and eliminating infection. An excessive inflammatory response can also damage the body's own substances. At present, in addition to the use of antibiotics for the cause, commonly used anti-inflammatory drugs are mainly steroidal and non-steroidal anti-inflammatory drugs. [0003] Alginate is the main component of the cell wall of brown algae. It is a linear anionic polysaccharide forme...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K31/7032A61K31/715A61P29/00A61P9/00A61P25/00A61P19/02A61P1/00A61P1/04A61P17/00A61P17/06
Inventor 耿美玉辛现良丁健
Owner GREEN VALLEY (SHANGHAI) PHARM CO LTD
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