Preparation method for antibacterial collagen

An antibacterial and collagen technology, which is applied in the field of preparation of antibacterial collagen, can solve problems such as difficult to achieve biological activity, inability to obtain antibacterial properties, difficult to dissolve, etc., and achieve good market prospects, not easy to lose, and long-lasting antibacterial effects

Active Publication Date: 2017-01-25
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, there is a huge difference in biological activity between collagen and collagen, and the latter is difficult to achieve the biological activity of the former; there is also a huge difference in the solubility of the two. Although collagen is hydrophilic, it is difficult to dissolve in neutral In aqueous solution, antibacterial collagen cannot be obtained by the above method

Method used

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  • Preparation method for antibacterial collagen
  • Preparation method for antibacterial collagen
  • Preparation method for antibacterial collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of antibacterial collagen, comprising the steps of:

[0033] (1) use 0.04mol / L barbiturate saline solution and 0.2mol / L hydrochloric acid aqueous solution preparation pH to be the buffer solution of 8.0;

[0034] Dissolving type I collagen extracted from bovine hide in a buffer solution, controlling the mass concentration of collagen to be 5%, to obtain a collagen solution;

[0035] (2) Add the modified triclosan with 1.5% collagen mass to the collagen solution in step (1), heat up to 35° C., and react for 24 hours to obtain an antibacterial collagen solution;

[0036] (3) The antibacterial collagen solution obtained in step (2) was dialyzed at 0-4° C. for 72 hours, and then freeze-dried to obtain the antibacterial collagen.

[0037] In the present embodiment, the modified triclosan has the following structure:

[0038]

[0039] The preparation process is as follows:

[0040] Dissolve triclosan in carbon tetrachloride solution, sulfonate with ...

Embodiment 2

[0042] A preparation method of antibacterial collagen, comprising the steps of:

[0043] (1) use the borax aqueous solution of 0.05mol / L and the boric acid aqueous solution preparation pH of 0.2mol / L to be the buffer solution of 7.8;

[0044] Dissolving the type I collagen extracted from the rat tail button in a buffer solution, controlling the mass concentration of the collagen to be 2.5%, to obtain a collagen solution;

[0045] (2) Add the modified triclosan with 5% collagen mass to the collagen solution obtained in step (1), heat up to 30° C., and react for 36 hours to obtain an antibacterial collagen solution;

[0046] (3) The antibacterial collagen solution obtained in step (2) was dialyzed at 0-4° C. for 48 hours, and then freeze-dried to obtain the antibacterial collagen.

[0047] In the present embodiment, the modified triclosan is a mixture with the following two structures:

[0048]

[0049] The preparation process is as follows:

[0050] Dissolve triclosan in ...

Embodiment 3

[0052] A preparation method of antibacterial collagen, comprising the steps of:

[0053] (1) Use 0.2mol / L glycine aqueous solution and 0.2mol / L sodium hydroxide aqueous solution to prepare a buffer solution with a pH of 9.2;

[0054] Dissolving the collagen I extracted from pigskin in a buffer solution, controlling the mass concentration of the collagen to be 2%, to obtain a collagen solution;

[0055] (2) Add the modified triclosan with 20% collagen quality to the collagen solution obtained in step (1), heat up to 37° C., and react for 24 hours to obtain an antibacterial collagen solution;

[0056] (3) The antibacterial collagen solution obtained in step (2) was dialyzed at 0-4° C. for 48 hours, and then freeze-dried to obtain the antibacterial collagen.

[0057] In the present embodiment, the modified triclosan has the following structure:

[0058]

[0059] The preparation process is as follows:

[0060] Dissolve triclosan in carbon tetrachloride solution, sulfonate with...

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Abstract

The invention relates to a preparation method for antibacterial collagen. The method comprises the following steps: (1) dissolving collagen in a buffer solution and controlling the mass concentration of collagen to be 0.1%-15%, thereby obtaining a collagen solution; (2) adding modified triclosan into the collagen solution obtained in the step (1), increasing the temperature to 25-50 DEG C and reacting for 0.1-48h, thereby obtaining an antibacterial collagen solution; (3) dialyzing the antibacterial collagen solution obtained in the step (2) for 0.5-96h at 0-4 DEG C, and then freeze-drying, thereby obtaining the antibacterial collagen. The antibacterial collagen prepared according to the method has the advantages that the antibacterial components are firmly combined with collagen through covalent bonds; the antibacterial effect is long-lasting; if the antibacterial collagen is prepared into the biomedical materials, including antibacterial collagen gel, collagen sponge, wound dressing, cytoskeleton of tissue engineering, and the like, the material has an antibacterial effect in the whole use period.

Description

technical field [0001] The invention belongs to the field of natural product modification and relates to a preparation method of antibacterial collagen. Background technique [0002] Collagen is the main component of animal skin, bone, connective tissue, etc. It has good physical and chemical properties and biocompatibility, and is widely used in food, medicine, cosmetics and other fields. Compared with collagen, only biomedical materials made of collagen molecules or oligomers that maintain a triple helix structure have excellent biological activity. Therefore, collagen is mainly used in the preparation of biomaterials, rather than its hydrolyzed product collagen. When collagen is used to prepare biomedical materials such as cell culture substrates, wound dressings, and artificial skin, its antibacterial and antiseptic properties have a very important impact on the application range and application effect of such materials. [0003] However, the current research mainly foc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08K5/42C07D251/26
CPCC07D251/26C08K5/42C08L89/00
Inventor 王玉路靳丽强
Owner 山东济清科技服务有限公司
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