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Alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and preparation method and application of alpha-glucosidase activity inhibition component

A technology for glucosidase activity inhibition, which is applied in the field of α-glucosidase activity inhibition components and its preparation, can solve the problems of insufficient development and utilization, and achieve the effects of less impurities, increased contact surface contact, and improved leaching speed

Active Publication Date: 2017-02-01
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yeyanghe is rich in resources, but has not been fully developed and utilized for a long time

Method used

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  • Alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and preparation method and application of alpha-glucosidase activity inhibition component
  • Alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and preparation method and application of alpha-glucosidase activity inhibition component
  • Alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and preparation method and application of alpha-glucosidase activity inhibition component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fresh Yeyanghe was crushed to a particle size of 420 μm, and methanol-water solution was used as the extractant, wherein the volume percentage of methanol in the methanol-water solution was 90%. According to the first material-liquid ratio of 20g:500mL, the extractant was mixed with the crushed Yeyanghe to obtain the extract. At 20°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 50 hours to obtain the crude extract of Yeyanghe. The crude extract was centrifuged and filtered, the supernatant was collected and separated, and the residue was extracted twice according to the above extraction method, and the supernatants obtained three times were combined, and the supernatants were placed in a water bath at 45°C. Rotary evaporation at a speed of 50 revolutions / min and a vacuum of 0.07 MPa to obtain a crude extract extract. The weight of the crude extract extract is 10% of the raw materials of Yeyanghe. The sample was loaded by wet met...

Embodiment 2

[0035] The fresh Yeyanghe was removed and cleaned, dried at 20°C and pulverized to a particle size of 580μm. The absolute ethanol-water solution was used as the extractant. The volume percentage of the absolute ethanol in the absolute ethanol-water solution was 70 %. According to the first material-to-liquid ratio of 100g:6000mL, the extractant is mixed with the crushed Yeyanghe to obtain the extract. At 30°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 20 hours to obtain the crude extract of Yeyanghe. The crude extract was subjected to centrifugal filtration, the supernatant was collected and separated, and the residue was extracted twice according to the above extraction method, and the supernatants obtained three times were combined. The supernatant was placed in a water bath at 55°C, 30 Rotary evaporation at a speed of revolution / min and a vacuum of 0.08 MPa to obtain a crude extract extract whose weight is 15% of Yeyanghe raw mate...

Embodiment 3

[0037] The fresh Yeyanghe was removed and cleaned, dried at 45°C, and crushed to a particle size of 500μm. The absolute ethanol-water solution was used as the extractant. The volume percentage of the absolute ethanol in the absolute ethanol-water solution was 95 %. According to the first material-to-liquid ratio of 60g:3200mL, the extractant was mixed with the crushed Yeyanghe to obtain the extract. At 25°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 35 hours to obtain the crude extract of Yeyanghe. The crude extract was subjected to centrifugal filtration, the supernatant was collected and separated, and the residue was extracted twice according to the above extraction method, and the supernatants obtained three times were combined. The supernatant was placed in a 50°C water bath, 40 Rotary evaporation at a speed of revolution / min and a vacuum of 0.09 MPa to obtain a crude extract extract, the weight of which is 20% of Yeyanghe raw m...

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Abstract

The invention relates to an alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and a preparation method and application of the alpha-glucosidase activity inhibition component, belonging to the fields of chemistry and chemical engineering. The preparation method comprises the following steps: crushing the habenaria ciliolaris kranzl until the particle size of the crushed habenaria ciliolaris kranzl is 420-580mu m; mixing an extracting agent and the crushed habenaria ciliolaris kranzl according to a first solid-liquid ratio of (20-100g): (500-6000mL), and extracting for 20-50h to obtain a crude habenaria ciliolaris kranzl extract; filtering, collecting supernatant, and concentrating to obtain extractum of the crude extract; dispersing the extractum of the crude extract into water according to a second solid-liquid ratio of 1g: (1-6mL), carrying out gradient elution on a sample solution in a macroporous adsorption resin chromatography column, and collecting and concentrating methyl alcohol elution peak with activity to obtain the activity inhibition component. The method is simple and easy to operate. The alpha-glucosidase activity inhibition component prepared by the method has stronger alpha-glucosidase inhibition activity, and has a better inhibiting effect especially for the activity of maltase.

Description

Technical field [0001] The invention relates to the field of chemistry and chemical engineering, and particularly relates to an α-glucosidase activity inhibitory component in Yeyanghe and its preparation method and application. Background technique [0002] Diabetes (Diabetes mellitus, DM) is a disease of endocrine and metabolic disorders caused by insufficient insulin content in the human blood. It is characterized by increased blood sugar and urine sugar, which can cause damage to multiple systems and organs. It is a human heart disease. The main causes of cerebrovascular disease, blindness, amputation and renal failure. A variety of hypoglycemic drugs have been developed clinically, such as insulin secretion promoters to improve glucose tolerance and insulin response levels, glucose metabolizers, aldose reductase inhibitors to prevent and delay the onset of diseases, and insulin sensitizers. Among them, α-glucosidase inhibitors have become a hot spot in the development and re...

Claims

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Application Information

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IPC IPC(8): A61K36/898A61P3/10
CPCA61K36/898A61K2236/333A61K2236/55
Inventor 高鸿苟安娜钟凯黄毅娜
Owner SICHUAN UNIV
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