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Harvesting method of Dunaliella salina

A Dunaliella salina, harvesting method technology, applied in the direction of single-celled algae, isolated microorganisms, etc., can solve the problems of affecting the economic benefits of enterprises, large damage to algae cells, and low harvesting efficiency, and achieve a large amount of one-time processing and damage The effect of extreme degree and improvement of recovery efficiency

Active Publication Date: 2017-02-15
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of applying this method is high, and the damage to algae cells is relatively large, and the harvesting efficiency is extremely low. Each centrifuge can only process 1m per hour. 3 algal liquid
This will greatly affect the economic efficiency of the enterprise

Method used

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  • Harvesting method of Dunaliella salina
  • Harvesting method of Dunaliella salina
  • Harvesting method of Dunaliella salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1: The initial salinity of harvesting by centrifugal separation is 15 Dunaliella salina experiment

[0070] Experimental materials and equipment: necessary drainage pipes such as water pumps, tube centrifuges, blood counting boards, etc.

[0071] Experimental conditions: normal temperature and pressure

[0072] Algae fluid volume: 1.8m for each experimental treatment 3

[0073] The initial cell number of algal liquid: 40×104 cells / ml, 60×104 cells / ml, 80×104 cells / ml in the three tests of 1#, 2# and 3# respectively

[0074] Reaction time: 1.8 hours per test

[0075] Experimental results:

[0076] ①. The recovery efficiency is P1#=72.8%, P2#=64.4%, P3#=60.3%;

[0077] ②. The quality of algae powder produced is: M1#=0.203㎏, M2#=0.221㎏, M3#=0.242㎏

Embodiment 2

[0078] Example 2: Flocculation experiment of algae liquid with an initial salinity of 12

[0079] Experimental materials and equipment: the volume is 2m 3 3 vats (up and down with multiple drainage outlets) labeled 1#, 2#, 3#, analytical grade NaOH (≥99%), scales, blood counting boards and necessary drainage pipe devices

[0080] Experimental conditions: normal temperature and pressure

[0081] Algae liquid volume: 1.8m in each vat 3 ;

[0082] The initial cell number of algae liquid: 40×104 cells / ml, 60×104 cells / ml, 80×104 cells / ml in 1#, 2#, 3# tanks respectively

[0083] Added NaOH quality: 3.6kg per vat

[0084] Response time: 4 hours

[0085] Experimental results:

[0086] ① Floating or settling conditions: the algal flocculation in the three vats all settled;

[0087] ②. Recovery factor: P1#=87.8%, P2#=86.2%, P3#=85.5%;

[0088] ③. Quality of algae powder: M1#=0.286㎏, M2#=0.334㎏, M3#=0.392㎏

Embodiment 3

[0089] Example 3: Flocculation experiment of algae liquid with an initial salinity of 13

[0090] Experimental material and equipment: same as embodiment 2

[0091] Experimental conditions: close to Example 2.

[0092] Experimental results:

[0093] ① Floating or settling conditions: the algal flocculation in the three vats all settled;

[0094] ②. Recovery factor: P1#=84.5%, P2#=81.5%, P3#=78.9%;

[0095] ③. Quality of algae powder: M1#=0.262㎏, M2#=0.310㎏, M3#=0.375㎏

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Abstract

The invention relates to a harvesting method of Dunaliella salina. The harvesting method comprises a first part of determining the pH (potential of Hydrogen) value of a Dunaliella salina algal solution; a second part of calculating the amount of sodium hydroxide used for regulating the pH value of a certain volume of the Dunaliella salina algal solution to be 12 to flocculate the Dunaliella salina in the algal solution; a third part of determining time needed by complete flocculation; a fourth part of collecting the Dunaliella salina; a fifth part of estimating a harvesting rate. In comparison with a centrifugal method in the prior art, by using the harvesting method of the Dunaliella salina, the treatment efficiency is obviously improved; the harvesting efficiency is drastically improved; the yield of algal powder is obviously promoted; meanwhile, the injuries to algal cells of the Dunaliella salina are reduced; the consumption of electric energy is decreased.

Description

technical field [0001] The invention relates to a harvesting method of Dunaliella salina, which is particularly suitable for the occasions where Dunaliella salina enterprises harvest algae cells. Background technique [0002] Dunalselal salana is a halophilic unicellular eukaryotic algae, one of the most salt-tolerant and halophilic eukaryotic unicellular organisms found so far, belonging to Chlorophyta, Euchlorophyceae, Volvox, Salinaceae, has no cell wall, and the cell surface has a positive charge. It is a representative algal species of Dunaliella species. Dunaliella salina can produce a large amount of carotenoids in a specific growth environment, among which the content of β-carotene is the highest, accounting for 14% of its dry weight. β-carotene belongs to tetraterpenoids, is an important natural food coloring, widely exists in plants, algae and fungi, and is the most abundant and researched carotenoid among all carotenoids. β-carotene has a strong function of scav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/02
Inventor 季祥王喜恩张凯凯张德荣巩东辉陈高
Owner INNER MONGOLIA UNIV OF SCI & TECH
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