Standard streptomycin samples of chicken, preparation method and application

A standard sample and streptomycin technology, applied in the field of analytical chemistry, can solve the problems affecting the reliability and validity of the results, the inability to reflect the accuracy of the method, and the effect of antioxidants on the results of use, etc., to achieve long shelf life, no preservatives and anti-corrosion Oxidizing agent, easy storage effect

Inactive Publication Date: 2017-02-15
辽宁省检验检疫科学技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the detection of streptomycin drug residues in the prior art cannot truly reproduce the extraction efficiency of the drug in animals after natural metabolism, it cannot reflect the accuracy of the method, which affe...

Method used

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  • Standard streptomycin samples of chicken, preparation method and application
  • Standard streptomycin samples of chicken, preparation method and application
  • Standard streptomycin samples of chicken, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation method of streptomycin standard sample in chicken, its concrete steps are as follows:

[0033] (1) Selection of experimental animals: Select healthy individual meat-eating white-feathered chickens of uniform size and average weight of 2 kg in a chicken farm, and the sexes are randomized. Quarantine at 25°C, feed once a day at 8:00 a.m., and select 18 of them for use after 3 days of domestication;

[0034] Drug feeding: 1.6g of streptomycin was weighed, added to 2.0kg of feed, stirred evenly, and fed to 18 domesticated chickens. Each chicken was fed with 50g of feed per day, once a day at 8 o'clock in the morning. The dosage is 20mg / kg body weight, continuously administered for 3 days;

[0035] (2) 3 days after the administration, that is, 3 hours after the last administration, take the chicken muscle after slaughter, grind it, and mix it to make meat mince;

[0036] (3) Vacuum freeze-drying: use vacuum freeze-drying machine (CHRiST Epsilon 2-6D) ...

Embodiment 2

[0041] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0042] 1) The body weight of the experimental animals is 1.5kg;

[0043]2) Weigh 0.2 g of streptomycin;

[0044] 3) The dosage of the three drugs is 3.3mg / kg body weight;

[0045] 4) Continuous administration for 5 days;

[0046] 5) Slaughter 3 hours after the last administration;

[0047] 6) Pre-freeze at -80°C for 0.5h, vacuum freeze at 0.01mbar for 8h, and quickly heat up and dry for 2h;

[0048] 7) Pass through a 50-mesh sieve;

[0049] 8) Store in cold storage at 5°C.

Embodiment 3

[0051] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0052] 1) The body weight of experimental animals is 2.5kg;

[0053] 2) Weigh 2.4 g of streptomycin;

[0054] 3) The dosage of the three drugs is 24mg / kg body weight;

[0055] 4) Continuous administration for 4 days;

[0056] 5) Slaughter 4 hours after the last administration;

[0057] 6) Pre-freezing at -30°C for 10 hours, vacuum freezing at 1.0 mbar for 15 hours, and rapid heating and drying for 10 hours;

[0058] 7) pass through 80 mesh sieve;

[0059] 8) Store in cold storage at 8°C.

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Abstract

The invention provides standard streptomycin samples of chicken, a preparation method and an application and belongs to the technical field of analytical chemistry. Chickens are fed with feed containing streptomycin, so that in-vivo tissue of live chickens contains the streptomycin, the chickens are killed after drug application, feathers and skin are removed, the chicken is taken and homogenized, minced meat is prepared and subjected to vacuum freeze drying, sieving, bottling and packaging, and standard samples of lyophilized chicken powder containing the streptomycin are obtained. The method solves the problem that treatment results such as extraction, purification and the like are more different from routine analysis samples due to the fact that the a target and matrix combining condition is incompletely consistent with a real detection sample, and the standard samples are material standard samples, are homogenous, stable, free of preservatives and antioxidants and easy to store and are used for proficiency testing of a streptomycin drug residue testing project, verification of a detection method, calibration of testing equipment as well as quality control and assessment of testing results.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to the preparation of a natural matrix standard sample of veterinary drug residues, in particular to a standard sample of streptomycin residue matrix freeze-dried powder in chicken and its preparation method and application. Background technique [0002] Streptomycin is an antibiotic extracted from the culture solution of Streptomyces griseus in 1944. Its molecular formula is C21H39N7O12, its molecular weight is 581, it is a white powder, water-soluble, relatively stable to heat at a pH value of 3 to 7, and has hydrochloric acid products such as salt and sulphate. Streptomycin belongs to aminoglycoside basic compound, is a kind of antibiotic organic base, can resist many bacteria effectively, it combines with tubercle bacillus ribonucleic acid proteosome protein, has played the effect of interfering with tubercle bacillus protein synthesis, thereby The effect of killing or inhibiti...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 王秋艳林维宣张宁王贵滨
Owner 辽宁省检验检疫科学技术研究院
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