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Method for preventing intestinal radiation damage and application of TPA

A radiation damage and application technology, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, digestive system, etc., can solve the problems of serious side effects, narrow treatment time window, etc., to slow down weight loss, prevent radiation damage, help effect on weight recovery

Inactive Publication Date: 2017-02-22
孙君重 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amifostine (free radical scavenger) is a classic drug currently approved for radiotherapy, which has shown certain effects in preventing clinical gastrointestinal radiotoxicity, but has severe side effects and a narrow treatment time window

Method used

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  • Method for preventing intestinal radiation damage and application of TPA
  • Method for preventing intestinal radiation damage and application of TPA
  • Method for preventing intestinal radiation damage and application of TPA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Experimental cells and culture conditions

[0020] The rat jejunum epithelial cell line (IEC-6) is an adherent cell, and its culture condition is: use H-DMEM medium, containing 10% FBS, 1% double antibody, 1U / ml regular insulin, placed at 37 ° C, 5 %CO 2 The cells were cultured in an incubator, and the medium was changed routinely for three days, passaged at 1:3-6, and 1 ml of trypsin was added during passage. Digest in the incubator for 2-3 minutes, do not over-digest, and stop digestion immediately after 80% of the adherent cells are fused. Cells in logarithmic growth phase were used for each experiment.

[0021] 2. Experimental animals and feeding conditions

[0022] SPF grade BALB / c mice, female, 6-8 weeks old, weighing 18-20 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2012-0001. Five standard feedstuffs were sterilized by ultraviolet irradiation in each cage, and the drinking water was...

Embodiment 2

[0040] Through human control experiments, three groups of radiotherapy patients were selected, of which the first group used amifostine to protect intestinal radiation, the second group did not use any protective drugs; the third group used 12-oxo-tetradecanoylphorbol-13- acetate (TPA). The experimental results showed that the intestinal mucosal cell death rates were 13.9%, 19.9%, and 14.1%, respectively, and the symptoms of acute intestinal injury (nausea, vomiting, and diarrhea) of the patients were: slight, obvious, and slight. The side effects were: serious, none, mild.

[0041] In summary, the beneficial effects of the present invention are:

[0042] The TPA of the present invention has anti-radiation effect, prevents massive death of small intestinal crypt stem cells caused by radiation damage, slows down weight loss and contributes to weight recovery.

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Abstract

The invention discloses a method for preventing intestinal radiation damage, wherein the intestinal stem cell genes Dclk1, Msi1, Lgr5, Bmi1 and Notch1 are up-regulated through TPA, the key signal proteins PKC-betaII and Erk1 / 2 as well as the protein p90RSK are further up-regulated, and the intestinal epithelial cell proliferation is promoted to prevent intestinal radiation damage.

Description

technical field [0001] The invention relates to a method for protecting intestinal radiation damage and application of TPA. Background technique [0002] In today's society, the development and utilization of nuclear energy is very extensive. As the possibility of nuclear incidents increases, it is particularly necessary and urgent to find new anti-radiation drugs that can alleviate the potentially fatal effects on vital organs such as bone marrow and gastrointestinal tract. In the medical field, radiotherapy is one of the most commonly used treatments for malignant tumors, but the adverse reactions caused by radiotherapy have brought huge physical and psychological harm to patients, and reducing the side effects of radiotherapy is still a clinical problem that needs to be solved urgently. The gastrointestinal tract and hematopoietic system are highly sensitive to radiation, and radiation-induced gastrointestinal syndrome and bone marrow suppression are the main causes of de...

Claims

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Application Information

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IPC IPC(8): A61K31/215A61P1/00A61P35/00
CPCA61K31/215
Inventor 孙君重梁耀杰王志红姚毅冰
Owner 孙君重
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