A kind of bacterial agent for high-efficiency treatment of penicillin wastewater and its preparation method and application
A technology for wastewater treatment and penicillin, which is applied in natural water treatment, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problems of complex preparation process and high production cost, and achieve guaranteed treatment effect, low cost and excellent preparation process simple and easy effects
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Embodiment 1
[0035] 1. Strain Isolation and Identification
[0036] This example demonstrates a preparation method of bacterial agent for high-efficiency treatment of penicillin wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:
[0037] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 0.5mm, the filling ratio is 1%, and then place it on a vortex shaker for 10 minutes , fully break up the sludge, and then let it stand for 10 minutes.
[0038] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 2% (mass concentration) sodium sulfate, culture at 20°C for 24 hours, and pick a single colony to a new Streak culture on the flat plate, pick and isolate and purify repeatedly, and expand the single strain in the liquid medium corresponding to the solid medium...
Embodiment 2
[0047] 1. Strain Isolation and Identification
[0048] This example demonstrates a preparation method of bacterial agent for high-efficiency treatment of penicillin wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:
[0049] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 1.0mm, and the filling ratio is 2% (accounting for the volume ratio of the centrifuge tube), and then place Vortex on a vortex shaker for 20 minutes to fully break up the sludge, and then let it stand for 30 minutes.
[0050] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 2% (mass concentration) sodium chloride, culture at 25°C for 32 hours, and pick a single colony to Streak culture on a new plate, pick and isolate and purify repeatedly, and expand the single str...
Embodiment 3
[0059] 1. Strain Isolation and Identification
[0060] This example uses the above method to demonstrate the preparation process of a bacterial agent for efficient treatment of tetracycline wastewater, and its process flow chart is as follows figure 1 As shown, the specific steps are:
[0061] (1) Collect 10mL of the mixed solution in the aeration tank of the biological sewage treatment plant, put it into a 50mL centrifuge tube, add glass beads with a diameter of 0.2mm, the filling ratio is 3%, and then place it on a vortex shaker for 20 minutes , fully break up the sludge, and then let it stand for 30 minutes.
[0062] (2) Take 1 mL of the supernatant after standing still for gradient dilution, and then spread it on a solid medium containing 10% (mass concentration) sodium sulfate, culture at 25°C for 48 hours, and pick a single colony to a new Stretch culture on the flat plate, pick and isolate and purify repeatedly, and expand the single strain in the liquid medium corres...
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Abstract
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