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Lactobacillus plantarum SG5 for producing gamma-aminobutyric acid

A technology of Lactobacillus plantarum and aminobutyric acid, applied in the field of microorganisms, can solve the problems of slow growth of fungi and cannot meet production needs, and achieve the effects of promoting growth hormone secretion, lowering cholesterol, and wide application prospects

Active Publication Date: 2017-02-22
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the gamma-aminobutyric acid-producing microorganisms isolated by the prior art are fungi, which grow slowly and cannot meet production needs

Method used

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  • Lactobacillus plantarum SG5 for producing gamma-aminobutyric acid
  • Lactobacillus plantarum SG5 for producing gamma-aminobutyric acid
  • Lactobacillus plantarum SG5 for producing gamma-aminobutyric acid

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Experimental program
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Embodiment 1

[0024] Isolation, screening and identification of embodiment 1 bacterial strain

[0025] Strain isolation: select fresh and complete mulberry leaves and rinse them with tap water first, then rinse them with sterile water in a sterile ultra-clean bench and dry them with sterile filter paper, then cut them into pieces; put the cut mulberry leaves in MRSG liquid Culture medium (2% v / v, 1 mg / mL sodium glutamate was added to MRS medium), cultured in a constant temperature incubator at 30°C for 2 days, and when bacteria grew around the mulberry leaf fragments, the bacteria were picked and placed in the MRS Separation and purification by streaking multiple times on solid medium; culture the purified strains on slant medium (MRS), pick a single colony and inoculate in fermentation medium TYG at 30°C for 48 hours, then do TLC qualitative, preliminary identification and separation can be obtained The strain can produce γ-aminobutyric acid.

[0026] Negative control: In order to check w...

Embodiment 2

[0039] The cultivation of embodiment 2 bacterial strains

[0040] Lactobacillus plantarum SG5 can grow on MRS medium, MRS medium (1000mL) is made up of 20g yeast extract, 20g glucose, 10g sodium succinate, 5mg / mL L-glutamic acid (0.5% v / v), 1mg phosphoric acid Pyridoxal (growth factor) and 1mg / mL Ca 2+ and the remaining amount of distilled water, adjust the pH value to 6.8-7.0, and autoclave at 121°C for 20 minutes.

[0041] 1. Activation of Lactobacillus plantarum SG5: Aseptic operation in a clean bench. Lactobacillus plantarum SG5 was picked with an inoculation loop, streaked with an inoculation loop on the MRS solid medium, and cultured in a 30°C incubator for 2 days.

[0042] 2. Preparation of fermentation samples of Lactobacillus plantarum SG5: Inoculate the isolated Lactobacillus plantarum SG5 in MRS liquid medium for 3 repetitions, place in 150r / min, 30°C constant temperature shaking incubator, and cultivate for 16-18h Take it out as seed liquid; inoculate the seed l...

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Abstract

The invention provides a lactobacillus plantarum SG5 for producing gamma-aminobutyric acid (GABA). The lactobacillus plantarum SG5 is preserved in the Guangdong Microbiological Culture Collection Center; the collection address is the Guangdong Institute of Microbiology; the preservation date is March 16, 2016; the preservation number is GDMCC No: 60020. The lactobacillus plantarum SG5 is used for fermentation, so that the content of GABA can reach 59 [mu]g / mL; the GABA has a variety of beneficial healthcare functions of lowering blood pressure, promoting growth hormone secretion, lowering cholesterol and the like, so as to provide basis for the development of new GABA-rich health-care food; the lactobacillus plantarum SG5 is applied to the development of foods and health-care products, and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a γ-aminobutyric acid-producing Lactobacillus plantarum SG5. Background technique [0002] γ-aminobutyric acid (GABA for short) is an inhibitory transmitter in the nervous center of mammals, which can regulate blood pressure, promote mental stability, promote blood flow in the brain, increase brain vitality, nourish nerve cells, increase Growth hormone secretion, invigorating the liver and kidney, promoting ethanol metabolism (sobering up) and other functions. In 2009, the Ministry of Health has officially approved GABA as a new resource food. Raw materials enriched with GABA can be used in the production of functional foods to reduce and prevent high blood pressure through diet. The methods of obtaining GABA include chemical synthesis and biological methods. The cost of chemical synthesis is relatively high, and chemical substances are likely to remain i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00C12R1/25
CPCC12N1/20C12P13/005C12N1/205C12R2001/25
Inventor 林健荣何梦秀陈芳艳钟杨生
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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