A kind of perilla seed antioxidant dipeptide and its preparation method and application

A technology for oxidizing dipeptides and anti-oxidizing peptides, which is applied in the field of food biology and can solve the problems of less research on protein development and utilization, and no reports on the pharmacology of active peptides.

Active Publication Date: 2019-12-17
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic and foreign researches on perilla seeds mainly focus on oil extraction and biological activity, while there are few researches on the development and utilization of protein rich in its by-products, and there are almost no reports on its active peptides or their pharmacological research at home and abroad.

Method used

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  • A kind of perilla seed antioxidant dipeptide and its preparation method and application
  • A kind of perilla seed antioxidant dipeptide and its preparation method and application
  • A kind of perilla seed antioxidant dipeptide and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The separation and purification of the antioxidant dipeptide in the present invention includes two steps of Sephadex G-25 gel filtration chromatography and reverse phase high performance liquid chromatography (RP-HPLC).

[0029]Preparation of perilla seed crude protein enzymatic hydrolysis product: first degrease perilla seed with petroleum ether, then use alkali extraction and acid precipitation method to obtain perilla seed crude protein, use alkaline protease to enzymatically hydrolyze perilla seed crude protein, use alkali Protease under its optimum enzymolysis conditions: pH 9.80, enzyme addition 3000U / g, substrate concentration 3% (w / v), hydrolysis time 5.00h, inactivated in boiling water bath for 10min, centrifuged at 12000rpm for 10min, take the The clear liquid is freeze-dried, which is the enzymatic hydrolyzate of perilla seed protein;

[0030] Sephadex G-25 Gel Filtration Chromatography: Dissolve the freeze-dried powder of perilla seed enzymatic hydrolyzate i...

Embodiment 2

[0035] The natural antioxidant dipeptide activity obtained in Example 1 is studied:

[0036] ABTS free radical scavenging effect: prepare 7 mmol / L ABTS with distilled water + solution and 2.45 mmol / L potassium persulfate solution (must be placed at room temperature for 16 h before use), ABTS + Mix with potassium persulfate solution at a volume ratio of 1:1, and dilute the mixture to the absorbance value A with pH 7.4, 5 mmol / L phosphate buffer solution before use 734 is 0.70±0.02. Take 0.5 mL of samples with different concentrations and 0.5 mL of ABTS radical solution, mix and let stand for 10 min, then measure the absorbance at 734 nm. Deionized water and reduced glutathione were used instead of samples as blank control and positive control, respectively. ABTS free radical scavenging activity is calculated according to the following formula (1):

[0037] (1)

[0038] In the formula, A 0 : Absorbance value of blank control group; A s : Absorbance value of the sample ...

Embodiment 3

[0058] The natural antioxidative dipeptide obtained in embodiment 1 is carried out cytotoxicity and antioxidant research:

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Abstract

The invention discloses an antioxidant dipeptide from fructus perillae, a method for preparing the dipeptide and application thereof. A sequence of the antioxidant dipeptide is Phe-Tyr (PF). The antioxidant dipeptide, the method and the application have the advantages that as shown in in-vitro experiments, ABTS, hydroxyl free radicals and superoxide anions can be effectively removed by the antioxidant dipeptide, and the antioxidant dipeptide is high in reactive oxygen species free radical scavenging capacity (ORAC); peroxidation of linoleic acid and rat hepatic lipids can be effectively suppressed by the antioxidant dipeptide; as proved by cell experiments, the antioxidant dipeptide with low concentration is safe for cells, and obvious effects of protecting the cells from oxidative injury can be realized by the antioxidant dipeptide; obvious effects of inhibiting cancer cells can be realized when HepG-2 cells are treated by the antioxidant dipeptide with high concentration; the antioxidant dipeptide is simple in structure and high in antioxidant activity and can be used as an excellent substitute for existing artificially synthesized antioxidants, the antioxidant dipeptide and the method have important value in the aspects of development and application of novel antioxidant healthcare product and food additives, and the like.

Description

technical field [0001] The invention relates to a perilla seed natural antioxidant dipeptide, a preparation method thereof and an application in the fields of food and health care products, belonging to the field of food biotechnology. Background technique [0002] Perilla seed, also known as perilla seed, is the dry and mature fruit of perilla frutescens, an annual herb of the genus Perilla in the family Lamiaceae. Perilla is native to China. There are wild species and cultivated species in North China, South China, Central China, Southwest China and Taiwan Province. It is one of the first 60 medicinal and edible plants approved by the Ministry of Health of my country. Perilla, as a traditional Chinese medicine in my country, was first recorded in "Compendium of Materia Medica" by Li Shizhen, a medical sage in the Ming Dynasty. Perilla seeds are rich in oil. Due to the limitation of processing technology, perilla seeds are mainly used to extract edible oil in my country's ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/065C12P21/06C07K1/20C07K1/16A23L33/18
CPCA23V2002/00C07K5/06078C12P21/06A23V2200/30A23V2200/308
Inventor 洪晶胡磊王惠敏孟春
Owner FUZHOU UNIV
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