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Method for synthesizing 1,3-stearin-2-glyceryl monooleate through enzyme method

A technology for the synthesis of oleic acid glyceride and enzymatic method, which is applied in the direction of fermentation, etc., to achieve the effects of easy control, cost reduction, and large-scale industrialization promotion

Active Publication Date: 2017-03-08
JIANGNAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, the traditional immobilized enzyme Lipozyme RM IM, immobilized enzyme Novozym 435, etc. are mostly used in the preparation of cocoa butter improvers at home and abroad, and the obtained SOS content is generally between 30% and 40%. The influence of the rate has not been reported in the relevant literature

Method used

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  • Method for synthesizing 1,3-stearin-2-glyceryl monooleate through enzyme method
  • Method for synthesizing 1,3-stearin-2-glyceryl monooleate through enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The batch enzyme reaction is carried out in a batch-type stirred tank reactor. In a solvent-free system, the molar ratio of high oleic sunflower oil and stearic acid is 1:12. By adding 10% immobilized enzyme NS40086, normal pressure The reaction was stirred at 70°C for 6h. After the reaction, the immobilized enzyme was removed from the reaction product by centrifugation. After the sample was prepared, it was analyzed by liquid chromatography, and the SOS content was determined to be 72.81% by comparison with the SOS standard. Such as figure 1 As shown, the triglycerides represented by the three peaks in the figure are OOO, SOO, and SOS. Under the high substrate molar ratio, the reaction conversion rate is high.

Embodiment 2

[0028] The batch enzyme reaction was carried out in a batch-type stirred tank reactor, the molar ratio of high oleic corn oil to methyl stearate was 1:4, and the ratio of added high oleic corn oil to n-hexane was 1:4 (w / v) by adding 10% immobilized enzyme NS40086, stirring the reaction at normal pressure 75°C for 6h. After the reaction, the immobilized enzyme was removed from the reaction product by centrifugal sedimentation. After the sample was prepared, it was analyzed by liquid chromatography, and the SOS content was determined to be 43.18% by comparison with the SOS standard.

Embodiment 3

[0030] The batch enzyme reaction is carried out in a batch-type stirred tank reactor. In a solvent-free system, the molar ratio of high oleic rapeseed oil and stearic acid is 1:2. By adding 10% immobilized enzyme NS40086, the normal pressure is 70 The reaction was stirred at ℃ for 4h. After the reaction, the immobilized enzyme was removed from the reaction product by centrifugation. After the sample was prepared, it was analyzed by liquid chromatography. The SOS content was determined to be 23.77% by comparison with the SOS standard. Such as figure 2 As shown, the triglycerides represented by the four peaks in the figure are OOL, OOO, SOO, and SOS. At low substrate molar ratios, the reaction conversion rate is low, resulting in low SOS yield.

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Abstract

The invention discloses a method for synthesizing 1,3-stearin-2-glyceryl monooleate through an enzyme method. High oleic acid vegetable oil and a stearic acid acyl donor are mixed at a certain substrate molar ratio. A certain amount of lipase is added within or without a solvent system. The reaction is carried out for 1-10 hours at 60-80 DEG C and a reaction product containing SOS is obtained. The method of the invention inhibits an acyl transfer side reaction by using the enzyme specificity, efficiency and by controlling the reaction conditions. The reaction conversion rate is very high, at the same time, the synthesis time is shortened. It is easy to control the whole process. The reaction conditions are mild, and the cost can be greatly reduced through the recovery and utilization of the enzyme. It is convenient for large scale industrialization popularization and the application potential is huge.

Description

technical field [0001] The invention belongs to the technical field of development of special fats and oils, and relates to a method for enzymatically synthesizing 1,3-distearic acid-2-oleic acid glyceride (SOS). Background technique [0002] The cocoa beans that produce cocoa butter grow within 20° north and south latitude of the tropical equator. Due to geographical and climatic conditions, the production of cocoa butter is far from meeting the market demand for chocolate in the world. In order to meet the demand of chocolate food, the research on cocoa butter substitutes has been vigorously developed, and the market prospect is broad, especially the demand in developing countries is increasing day by day. Secondly, in my country's existing industry standards and national standards, cocoa butter substitutes have 10-15% room for expansion. Therefore, the vast consumer market and huge profit margins make cocoa butter substitutes have broad development value. [0003] Coco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 王小三陈洋陆继源金青哲王兴国
Owner JIANGNAN UNIV
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