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Preparation method of microbial bactericide

A bactericide and microbial technology, which is applied in the field of microbial bactericide preparation, can solve the problems of narrow bactericidal spectrum, single application range, and restrictions on the wide application of microbial preparations, and achieve a large number of total bacteria, a high conversion rate of spores, and enhanced plant resistance. The effect of reverse ability

Inactive Publication Date: 2017-03-15
中弘新缘生物科技(天津)有限公司
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Existing microbial fungicides have a relatively single application range in the actual application process, and their bactericidal spectrum is relatively narrow
In addition, there are many deficiencies in existing products in terms of industrial application, environmental adaptability and stress resistance, which limit the wide application of microbial preparations in crop sterilization

Method used

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Embodiment Construction

[0015] The present invention will be further elaborated below in conjunction with embodiment.

[0016] A kind of preparation method of microbial bactericide, concrete steps are:

[0017] Step 1: Bacillus subtilis slant culture, the temperature of the Bacillus subtilis slant culture is 35 ℃ ~ 42 ℃, the time is 42h ~ 52h, the formula of the Bacillus subtilis slant culture medium is: sodium chloride 0.2% ~ 0.8 %, beef extract 0.5% to 2%, peptone 0.2% to 0.8%, agar powder 1% to 2%, the rest is water, adjust the pH value to 7.0 to 8.5, pour the slope after sterilization, and inoculate and cultivate;

[0018] Step 2: Bacillus subtilis shake flask culture, the temperature of the Bacillus subtilis shake flask culture is 35 ℃ ~ 42 ℃, the time is 30h ~ 36h, the stirring speed is 200rpm, the formula of the Bacillus subtilis shake flask culture medium is : Corn gluten powder 0.2%~0.8%, peptone 0.5%~2%, starch 1%~2%, glucose 0.5%~2%, potassium dihydrogen phosphate 0.05%~0.15%, the rest is...

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Abstract

The invention relates to the technical field of agricultural microbial agents, in particular to a preparation method of a microbial bactericide. The specific steps include: 1. bacillus subtilis slant culture; 2. bacillus subtilis shaking culture; 3. bacillus subtilis seed culture; 4. bacillus subtilis fermentation culture; and 5. adding of 0.001%-0.002% of sterilized magnesium sulfate 2h-6h before discharge of a bacillus subtilis fermentation tank. The microbial bactericide prepared by the method provided by the invention has high spore conversion rate and total bacteria count, can inhibit pathogen implantation when used for controlling plant diseases, produce a variety of antibiotics and protein antibacterial substances, and induce plants to generate resistance, has broad-spectrum antibacterial activity, and can enhance the adverse resistance of plants, effectively control crop diseases, and improve the disease resistance, drought resistance and cold resistance of plants.

Description

technical field [0001] The invention relates to the technical field of agricultural microbial bactericides, in particular to a method for preparing microbial bactericides. Background technique [0002] Plant diseases and insect pests are the enemies of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the average annual loss of production due to plant diseases is 15% to 20% of the total output. For a long time, the preparations used for crop disease control are generally chemical pesticides. Frequent use of chemical pesticides for a long period of time has led to increased resistance of pathogenic bacteria and gradually reduced the effectiveness of chemical pesticides. At present, the main solution is to increase the dosage of medicines, which directly leads to the increase of agricultural costs; the strengthening of the resistance of pathogenic bacteria and the difficulty of prevention and control; th...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P1/00A01P3/00C12N1/20C12R1/125
CPCA01N63/10C12N1/20
Inventor 常立新
Owner 中弘新缘生物科技(天津)有限公司
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