Application of probiotic microcapsule in preparation of drugs for inhibition of pathogenic bacterial biofilm
A biofilm, microcapsule technology, used in microcapsules, capsule delivery, antibacterial drugs, etc.
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Embodiment 1
[0008] Step 1: Put 5×10 8 1 lactic acid bacteria cells were suspended in 1mL sodium alginate solution (8 g / L), and the droplets were sprayed into 1.0 mol / L CaCl by electrostatic droplet method. 2 solution, reacted for 30 min, and the obtained calcium alginate gel microspheres had a particle size of 300 μm, and the particle size distribution was normal, with good monodispersity;
[0009] The second step: immerse the above-mentioned hydrogel microspheres in a chitosan solution with a mass concentration of 0.5%, and the volume ratio of the microspheres to the chitosan solution is 1:10, and react for 10 min. At this time, alginic acid embedded with lactic acid bacteria is obtained. Sodium-chitosan microcapsules are taken out and washed with normal saline;
[0010] The third step: immerse the microcapsules in the second step in 40-70 mmol / L sodium citrate solution to liquefy the alginate gel inside the microcapsules, react for 5 min, take them out and wash them with normal saline,...
Embodiment 2
[0014] Step 1: Put 5×10 8 Lactobacillus rhamnosus cells were suspended in 1 mL mixed with CaCO 3 In the sodium alginate solution (8 g / L), the volume ratio of sodium alginate to oil phase was 1:5, the amount of surfactant added was 0.5% (v / v), emulsified for 20 min, and HAC was added to initiate gelation Reaction; Obtain calcium alginate hydrogel microspheres;
[0015] The second step: immerse the above-mentioned hydrogel microspheres in a chitosan solution with a mass concentration of 0.3%, and the volume ratio of the microspheres to the chitosan solution is 1:10, and react for 20 min. At this time, rhamnose-embedded Sodium alginate-polycation microcapsules of Lactobacillus, taken out and washed with physiological saline;
[0016] The third step: immerse the microcapsules in the second step in 40-70 mmol / L sodium citrate solution to liquefy the alginate gel inside the microcapsules, react for 5 min, take them out and wash them with normal saline, and at this time the inner ...
Embodiment 3
[0020] Step 1: Put 5×10 8 Streptococcus lactis cells were suspended in 10 mL of sodium alginate solution (8 g / L), the volume ratio of sodium alginate to oil phase was 1:10, and the amount of surfactant added was 0.1 (v / v) and emulsified for 10 min. Add CaCl 2 A gelation reaction is initiated to obtain calcium alginate hydrogel microspheres.
[0021] The second step: immerse the above-mentioned hydrogel microspheres in the polylysine solution with a mass concentration of 0.3%, the volume ratio of the microspheres to the polylysine solution is 1:10, and react for 15 minutes. Sodium alginate-polylysine microcapsules of cocci, taken out and washed with physiological saline;
[0022] Step 3: Dilute the overnight cultured Staphylococcus aureus suspension with BP medium to a concentration of 10 8 CFU / mL, take the microcapsules of Streptococcus lactis and 1 nM signal molecules prepared in the second step with the same number of cells and mix them with the aforementioned diluted St...
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