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Reduction responsiveness nanoscale graphene oxide coated medicine carrying mesoporous silicon dioxide nano particle and preparation method thereof

A technology of mesoporous silica and nano-graphite oxide, which can be used in pharmaceutical formulations, drug combinations, anti-tumor drugs, etc., can solve problems such as the preparation of reduction-responsive drug controlled release systems, and achieve rapid reduction-responsive release, The effect of strong biocompatibility and simple operation

Inactive Publication Date: 2017-03-15
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, there are still no reports on the preparation of reduction-responsive drug release systems by combining nano-graphene oxide with mesoporous silica nanoparticles.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] The preparation method of the reduction-responsive nano-graphene oxide-coated drug-loaded mesoporous silica nanoparticles comprises the following steps:

[0036] A. Reacting mesoporous silica capable of loading drugs with a silane coupling agent, and grafting amino groups on its surface to prepare aminated mesoporous silica;

[0037] B. reacting the aminated mesoporous silica obtained in step A with a compound containing disulfide bonds, amino groups and carboxyl groups at the same time, and grafting disulfide bonds on the surface to prepare mesoporous silica containing disulfide bonds;

[0038] C. The mesoporous silica containing disulfide bonds obtained in step B is physically impregnated to form drug-loaded mesoporous silica;

[0039] D. The amino group on the drug-loaded mesoporous silica obtained in step C is amide-reacted with the carboxyl group on the surface of nano-graphene oxide to obtain reduction-responsive nano-graphene oxide-coated drug-loaded mesoporous s...

Embodiment 1

[0041] a. Ultrasonic disperse 0.1 g of mesoporous silica nanoparticles (MSNs) with a particle size of 100 nm and a mesoporous diameter of 3 nm into 10 ml of distilled water, then add 100 μl of acetic acid and 25 μl of γ-aminopropyl triethoxy base silane, stirred at room temperature for 24 hours, centrifuged and washed to obtain aminated mesoporous silica nanoparticles (MSNs-NH 2 );

[0042] b. Disperse 0.01 g of oxidized glutathione in 10 ml of PBS buffer solution (pH=5.0), then add 50 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide Aqueous solution of hydrochloride, stirred at room temperature for 1 hour so as to activate the carboxyl group on the oxidized glutathione; 0.04g MSNs-NH 2 Added into the above solution, stirred at room temperature for 24 hours, centrifuged and washed to obtain mesoporous silica nanoparticles containing disulfide bonds (MSNs-SS-NH 2 );

[0043] c. Diffusion of camptothecin molecules into MSNs-SS-NH by a simple physical impregnation method 2...

Embodiment 2

[0046] a. Ultrasonic disperse 0.1 g of mesoporous silica nanoparticles (MSNs) with a particle size of 150 nm and a mesoporous diameter of 5 nm into 10 ml of distilled water, then add 100 μl of acetic acid and 25 μl of γ-aminopropyltrimethoxy Silane, stirred at room temperature for 24 hours, centrifuged and washed to obtain aminated mesoporous silica nanoparticles (MSNs-NH 2 );

[0047] b. disperse 0.01g of cystine in 10ml of PBS buffer solution (pH=5.0), then add 50mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Aqueous solution, stirred at room temperature for 1 hour in order to activate the carboxyl group on cystine; 0.04g MSNs-NH 2 Added into the above solution, stirred at room temperature for 24 hours, centrifuged and washed to obtain mesoporous silica nanoparticles containing disulfide bonds (MSNs-SS-NH 2 );

[0048] c. Diffusion of doxorubicin molecules into MSNs-SS-NH by a simple physical impregnation method 2 mesoporous channels; then MSNs-SS-NH ...

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Abstract

The invention relates to a reduction responsiveness nanoscale graphene oxide coated medicine carrying mesoporous silicon dioxide nano particle and a preparation method thereof. The reduction responsiveness nanoscale graphene oxide coated medicine carrying mesoporous silicon dioxide nano particle consists of mesoporous silicon dioxide nano particles and nanoscale graphene oxide, wherein the mesoporous silicon dioxide nano particles are modified by a compound which simultaneously contains a disulfide bond, an amino group and carboxyl and can load medicines, and the nanoscale graphene oxide coats the mesoporous silicon dioxide nano particles. The preparation method comprises the following steps: adding the mesoporous silicon dioxide nano particles which are modified by the compound which simultaneously contains the disulfide bond, the amino group and the carboxyl and can load medicines into carboxyl activated nanoscale graphene oxide aqueous solution, stirring at room temperature to generate amidation reaction, carrying out centrifugal washing, and carrying out freeze drying to obtain a medicine controlled release material which is modified by the nanoscale graphene oxide and has reduction responsiveness. The carrier material of the invention has good biocompatibility and high medicine loading capacity, and the controlled release of the medicine can be realized by the reduction responsiveness of the disulfide bond, the medicine hardly releases around normal cells but quickly releases under the microenvironment of tumor cell reduction, and the nano particle has important physiological significance and research value.

Description

technical field [0001] The invention relates to a drug-loaded controlled-release material and a preparation method thereof, in particular to a reduction-responsive nanometer graphene oxide-coated drug-loaded mesoporous silicon dioxide nanoparticle and a preparation method thereof. Background technique [0002] At present, malignant tumors have become a killer that endangers human health, and its incidence is increasing year by year, and it is showing a younger trend. For malignant tumors, the main treatment methods are chemotherapy, surgery and radiotherapy. At present, chemotherapy has become the most commonly used therapy, but due to the large dosage of chemotherapy drugs and certain harm to normal cells, the curative effect of chemotherapy is low. [0003] As an emerging science and technology, nanotechnology is widely used in the medical field, especially in the development of anti-cancer treatment methods and the preparation of nano-drug carriers, and has played an ext...

Claims

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Application Information

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IPC IPC(8): A61K47/04A61K31/4745A61K31/704A61K31/192A61K31/375A61K31/337A61P35/00
CPCA61K47/02A61K31/192A61K31/337A61K31/375A61K31/4745A61K31/704
Inventor 崔学军董林林施超孙悦歆
Owner JILIN UNIV
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